Comparison of methods for antimicrobial susceptibility testing and MIC values for pleuromutilin drugs for Brachyspira hyodysenteriae isolated in Germany

Rohde, Judith; Keßler, Martina; Baums, Christoph Georg; Amtsberg, G

doi:10.1016/j.vetmic.2004.03.023

Cited by 48 publications

(71 citation statements)

References 14 publications

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“…Hidalgo et al (2011) identified a mutation in the 23S gene at position 2059 associated with resistance to tylvalosin. For lincomycin, the MIC50 was higher compared with that reported in the literature (Karlsson et al 1999, Karlsson et al 2002, Karlsson et al 2003, Rohde et al 2004, Hidalgo et al 2011, Zmudzki et al 2012, Mirajkar & Gebhart 2013, however, the MIC 90 was similar to those obtained by Hidalgo et al (2009) and Alvarez et al (2013).…”

Section: Discussionsupporting

confidence: 84%

“…For doxycycline, the MIC50 values were comparable to those found by Zmudzki et al (2012) and Alvarez et al (2013), but the MIC90 was elevated compared with the previously described values (Karlsson et al 2002, Rohde et al 2004, Pringle et al 2007, Hidalgo et al 2009, 2011, Zmudzki et al 2012, Alvarez et al 2013, Mirajkar & Gebhart 2013. Decreased susceptibility to doxycycline has been associated with a mutation at position 1058 of the 16S rRNA gene for B. hyodysenteriae (Pringle et al 2007).…”

Section: Discussionsupporting

confidence: 79%

“…The MIC results presented here were compared with the literature involving the same broth microdilution technique (Rohde et al 2004). The results for tylosin were similar to those found in the literature regarding both the MIC50 and MIC90 (Table 1).…”

Section: Discussionsupporting

confidence: 79%

“…Identifying the sensitivity pattern of Brazilian strains is important for establishing swine dysentery control and eradication programs, mainly due to the emergence of B. hyodysenteriae strains with reduced susceptibility to multiple antimicrobials, based on microbiological cut-off points, which was observed in both the present work and other studies in different countries (Karlsson et al 2002, Rohde et al 2004, Hidalgo et al 2009, 2011, Zmudzki et al 2012, Alvarez et al 2013, Mirajkar & Gebhart 2013. In addition, low sensitivity is relevant for resistance monitoring programmes as it is an indication of early resistance and allows the implementation of measures to prevent the emergence of strains with high resistance (Pringle et al 2012).…”

Section: Discussionsupporting

confidence: 61%

“…The results for tylosin were similar to those found in the literature regarding both the MIC50 and MIC90 (Table 1). High resistance to tylosin was expected based on the selective pressure caused by the widespread use of this drug in therapeutic and prophylactic programmes in recent years (Karlsson et al 2002, Rohde et al 2004, Hidalgo et al 2009, 2011, Zmudzki et al 2012, Alvarez et al 2013, Mirajkar & Gebhart 2013. The mechanism of tylosin resistance is related to mutations in the 23S ribosomal RNA gene at position 2058, inhibiting its binding (Karlsson et al 1999(Karlsson et al , 2004.…”

Section: Discussionmentioning

confidence: 99%

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Minimum inhibitory concentration of Brazilian Brachyspira hyodysenteriae strains

et al. 2017

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The objectives of this study were to characterize Brachyspira hyodysenteriae isolates and to evaluate the antimicrobial susceptibility patterns of strains obtained from pigs in Brazil based on the minimal inhibitory concentration test (MIC). The MIC was performed for 22 B. hyodysenteriae isolates obtained from 2011 to 2013 using the following antimicrobial drugs: tylosin, tiamulin, valnemulin, doxycycline, lincomycin and tylvalosin. Outbreaks of swine dysentery were diagnosed based on clinical presentation, bacterial isolation, gross and microscopic lesions, duplex PCR for B. hyodysenteriae and B. pilosicoli and nox gene sequencing. All obtained MIC values were consistently higher or equal to the microbiological cut-off described in the literature. The MIC 90 values for the tested drugs were 8μg/ml for doxycycline, >4μg/ml for valnemulin, 8μg/ml for tiamulin, 32μg/ml for tylvalosin, >64μg/ml for lincomycin and >128μg/ml for tylosin. These results largely corroborate those reported in the literature. Tiamulin, doxycycline and tylvalosin showed the lowest MIC results. All of the samples subjected to phylogenetic analysis based on the nox gene sequence exhibited similar results, showing 100% identity to B. hyodysenteriae. This is the first study describing the MIC pattern of B. hyodysenteriae isolated in Brazil.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionsupporting

confidence: 79%

Section: Discussionsupporting

confidence: 79%

Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Minimum inhibitory concentration of Brazilian Brachyspira hyodysenteriae strains

et al. 2017

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Synthesis, structure, and antibacterial evaluation of new N‐substituted‐3‐amino‐ 5‐oxo‐4‐phenyl‐2,5‐dihydro‐1H‐pyrazole‐1‐carbothioamides

Pitucha¹,

Mazur²,

Kosikowska³

et al. 2010

Heteroatom Chemistry

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Metabonomics-based analysis of Brachyspira pilosicoli 's response to tiamulin reveals metabolic activity despite significant growth inhibition

et al. 2017

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Pathogenic anaerobes Brachyspira spp. are responsible for an increasing number of Intestinal Spirochaetosis (IS) cases in livestock against which few approved treatments are available. Tiamulin is used to treat swine dysentery caused by Brachyspira spp. and recently has been used to handle avian intestinal spirochaetosis (AIS). The therapeutic dose used in chickens requires further evaluation since cases of bacterial resistance to tiamulin have been reported. In this study, we evaluated the impact of tiamulin at varying concentrations on the metabolism of B. pilosicoli using a H-NMR-based metabonomics approach allowing the capture of the overall bacterial metabolic response to antibiotic treatment. Based on growth curve studies, tiamulin impacted bacterial growth even at very low concentration (0.008 μg/mL) although its metabolic activity was barely affected 72 h post exposure to antibiotic treatment. Only the highest dose of tiamulin tested (0.250 μg/mL) caused a major metabolic shift. Results showed that below this concentration, bacteria could maintain a normal metabolic trajectory despite significant growth inhibition by the antibiotic, which may contribute to disease reemergence post antibiotic treatment. Indeed, we confirmed that B. pilosicoli remained viable even after exposition to the highest antibiotic dose. This paper stresses the need to ensure new evaluation of bacterial viability post bacteriostatic exposure such as tiamulin to guarantee treatment efficacy and decrease antibiotic resistance development.

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Comparison of methods for antimicrobial susceptibility testing and MIC values for pleuromutilin drugs for Brachyspira hyodysenteriae isolated in Germany

Cited by 48 publications

References 14 publications

Minimum inhibitory concentration of Brazilian Brachyspira hyodysenteriae strains

Minimum inhibitory concentration of Brazilian Brachyspira hyodysenteriae strains

Synthesis, structure, and antibacterial evaluation of new N‐substituted‐3‐amino‐ 5‐oxo‐4‐phenyl‐2,5‐dihydro‐1H‐pyrazole‐1‐carbothioamides

Metabonomics-based analysis of Brachyspira pilosicoli 's response to tiamulin reveals metabolic activity despite significant growth inhibition

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