Comparison of methods for obtaining doubled haploids of carrot

Abstract

Doubled haploid lines of carrot can be obtained through androgenesis in anther cultures and in isolated microspore cultures. The two methods were compared using three carrot cultivars ('Kazan F1' , 'Feria F1' , and 'Narbonne F1') at the androgenesis induction stage, during plant regeneration from embryos, and during acclimatization of androgenetic plants as well as their characterization. It was found that cultivar was the main factor affecting the efficiency at each stage of plant production in both anther and isolated microspore cultures. The efficiency of androgenesis in anther cultures of 'Feria F1' was considerably higher in comparison with isolated microspore cultures, and more plants were obtained from the embryos of androgenesis-cultured plants. In 'Kazan F1' and 'Narbonne F1' , more acclimatized androgenetic plants were produced from anther cultures. Ploidy assessment of acclimatized plants of 'Narbonne F1' showed that the majority of the plants in the population derived from anther cultures had a doubled chromosome (DH) set. On the other hand, the majority of plants obtained from isolated microspore cultures were haploids. When assessing homozygosity, it was found among plants obtained in anther cultures that the percentage of homozygotes for phosphoglucose isomerase (PGI) and aspartate aminotransferase (AAT) depended on the cultivar. In contrast, the majority of plants derived from isolated microspore cultures were homozygous regardless of cultivar.

Haploid Embryogenesis in Isolated Microspore Culture of Carrots (Daucus carota L.)

“…Carrots are among those plants that require exogenous growth regulators for the induction of embryogenesis. Andersen et al (1990) [ 32 ], Goreska et al (2005) [ 5 ], Goreska et al (2010) [ 9 ], Li et al (2013) [ 11 ], and Kiszczak et al [ 33 ] used 2.4 D in combination with NAA to induce embryogenesis. Hu et al (1993) [ 3 ] and Matsubara et al (1995) [ 8 ] for this purpose added 2.4 D and kinetin to the media, while Tyukavin et al (1999) [ 4 ] added only 2.4 D. We obtained positive results when using 0.2 mg/L of 2.4 D and kinetin in the culture of microspores of carrot varieties Nantskaya 4 and Chantenay.…”

Carrot: Breeding and Genomics

“…capillifolius (Gilli) Arbizu, were fused (Dudits et al, 1977b). The cultivation of unfertilized ovules (Kielkowska and Adamus, 2010) and the production of haploids from anther cultures have been reported (Andersen et al, 1990;Li et al, 2013;Kiszczak et al, 2017). Using somatic embryos produced in suspension cultures and encapsulated with alginic acid, artificial carrot seeds have been successfully developed (Liu et al, 1992;Latif et al, 2007).…”

GENERATION OF Rubus arcticus L. HAPLOIDS THROUGH in vitro MICROSPORE CULTURE TECHNIQUE

“…Работы, посвященные проблеме получения гаплоидных растений, объединяет общий план построения опытов: воздействие повышенными и/или пониженными температурами, химическая обработка донорных растений, изменение состава питательной среды (регуляторов роста, углеводных составляющих), подбор возрастной стадии развития пыльника и микроспоры. К настоящему времени опубликованы методики получения гаплоидных растений пшеницы (5,6), капусты (7)(8)(9), рапса (10), риса (11), моркови (12)(13)(14). Работы, посвященные получению гаплоидных растений R. arcticus L. в культуре микроспор, отсутствуют.…”