Comparison of Methods for the Isolation of Thermotolerant Campylobacter from Poultry

Abstract

Human campylobacteriosis has become the major cause of foodborne gastroenteritis in industrialized countries. Although there have been numerous studies investigating the prevalence of Campylobacter in animals and raw meat, sensitive and low-cost detection methods are needed to implement effective control measures during primary production and to use as tools in risk assessment studies. Thermophilic Campylobacter spp. in naturally contaminated (n = 64) and inoculated (n = 16) broiler samples were detected using two International Organization for Standardization (ISO)-approved methods. Both enrichment broths (those of Preston and of Park and Sanders) were inoculated with (i) Campylobacter jejuni ATCC35921, (ii) boneless breast from broilers, (iii) boneless breast rinse solution, (iv) boneless breast rinse solution inoculated with C. jejuni ATCC35921 before centrifugation, and (v) boneless breast rinse solution inoculated with C. jejuni ATCC35921 after centrifugation. The results indicated that the Park and Sanders broth was superior to the Preston broth for recovery of Campylobacter spp., and no significant differences (P > 0.05) were found between ISO (meat pieces) and modified ISO (centrifuged chicken rinse solution) methods for the detection of Campylobacter spp.

Campylobacter jejuni: A Review of its Characteristics, Pathogenicity, Ecology, Distribution, Subspecies Characterization and Molecular Methods of Detection

“…Park and Sanders broth (PSB) has been reported to be superior to Preston broth (PB) for enrichment recovery of Campylobacter jejuni from chicken tissue and rinse solution (Tangvatcharin et al, 2005). The authors speculated that the difference may be due to the improved recovery of sublethaly injured cells in PSB, which consists of a rich basal medium supplemented with yeast extract, sodium citrate, and oxygen-quenching ingredients such as sodium pyruvate.…”

Prevalence and Characteristics of Campylobacter Species Isolated from Gallbladder of Slaughtered Sheep in Van (Eastern) Turkey

“…They cause acute gastroenteritis in human and enteritis in animals. C. jejuni is recognized as a causative agent of avian hepatitis of fowl (Butzler 2004;Tangvatcharin et al 2005).…”

COMPARATIVE ASSESSMENT OF STANDARD CULTURE AND REAL‐TIME POLYMERASE CHAIN REACTION TO DETECT CAMPYLOBACTER JEJUNI IN RETAIL CHICKEN SAMPLES

“…Molecular genotype‐based methods represent rapid and sensitive alternatives to phenotype‐based methods for Campylobacter detection (Dickins et al. 2002; Tangvatcharin et al. 2005).…”

“…The identification of C. jejuni and C. coli from foods, stools or clinical samples using conventional culture-based methods may take up to 5 days. Molecular genotype-based methods represent rapid and sensitive alternatives to phenotype-based methods for Campylobacter detection (Dickins et al 2002;Tangvatcharin et al 2005).…”