Comparison of morphology and genetic diversity between broodstock and hybrid offspring of oriental river prawn, Macrobrachium nipponense based on morphological analysis and SNP markers

Abstract

Summary Hybridization is an effective method for the genetic improvement of farmed species. In this study, three broodstock populations (Changjiang, CJ, Dongting, DT, and Dianshan, DS) of oriental river prawn, Macrobrachium nipponense were used, and DS was used as the female broodstock. Through three‐line hybridization, two hybrid populations were finally obtained. The F3 generation of the broodstock population and the F1 generation of the hybrid population were cultured indoors for 3 months. Through morphological analysis (cluster analysis, discriminant analysis and path analysis) it was found that the hybrid population and the broodstock had some differences, but not enough to reach the subspecies level, and the dominant traits exhibited differentiation and reorganization. This study identified SNP genetic markers, carried out systematic evolution analysis and genetic diversity analysis and found that the nucleotide diversity π and heterozygosity Het of the hybrid population were higher than those of the broodstock. Among broodstocks, the differentiation index (Fst) of SCD and SDC was smallest (0.055). This research provides some valuable reference for genetic breeding.

Comparison of lipid metabolism between broodstock and hybrid offspring in the hepatopancreas of juvenile shrimp (Macrobrachium nipponense): Response to chronic ammonia stress

“…In our previous study, we reported that a hybrid population of M . nipponense was significantly better than the parent population with respect to growth performance and morphological and genetic characteristics (Li, Jiang et al, 2021; Li, Fan et al, 2021). In this study, after 28 days of chronic ammonia exposure, the survival rates of the SCD group were significantly higher than those of the DS group at the same ammonia concentrations.…”

“…nipponense with increasing ammonia concentrations, which was consistent with previous studies (Shan et al, 2019; Wang et al, 2021). LPS, FAS and ACC play important roles as rate‐limiting enzymes in fatty acid synthesis (Fouad, 2013; Li, Liu et al, 2021; Li, Jiang et al, 2021; Li, Fan et al, 2021). CPT‐1, as a lipid metabolism enzyme, participates in β‐ oxidation and fatty acid transport through the formation of lipoylcarnitine in the mitochondrial matrix (Kjær et al, 2008).…”

Comparison of immune defense and antioxidant capacity between broodstock and hybrid offspring of juvenile shrimp (Macrobrachium nipponense): Response to acute ammonia stress

“…During the breeding process, commercial feed (5% of shrimp weight) was provided at 08:00 and 18:00. The three‐line hybrid strategy was published in our previous studies (Li et al, 2021a, 2021b).…”

“…In previous studies, we compared morphology, genetic diversity, growth performance, and biochemical components between parent and hybrid offspring in the oriental river prawn, M . nipponense (Li et al, 2021a, 2021b). However, there are a limited number of studies on germplasm comparisons between native and hybrid shrimps under ammonia stress in oriental river prawn.…”

Comparison of detoxification capacity between broodstock and hybrid offspring in the gills of juvenile shrimp ( Macrobrachium nipponense ): Response to chronic ammonia stress

“…During the breeding process, we provided feed at 8:00 and 18:00. The three‐line hybrid strategy used was described previously (Li, Fan, et al, 2021a; Li, Jiang, et al, 2021b).…”