Comparison of optomagnetic and AC susceptibility readouts in a magnetic nanoparticle agglutination assay for detection of C-reactive protein

Fock, Jeppe; Parmvi, Mattias; Strömberg, Mattias; Svedlindh, Peter; Donolato, Marco; Hansen, Mikkel Fougt

doi:10.1016/j.bios.2016.07.088

Cited by 37 publications

(26 citation statements)

References 24 publications

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“…For example, light absorption of magnetic particles and clusters of magnetic particles may differ when exposed to an oscillating magnetic field. Fock et al, compared the performance of a C-reactive protein (CRP) biosensor with a newly proposed optomagnetic and alternating current (AC) susceptibility readout [ 217 ]. Both methods detect agglutination of magnetic particles.…”

Section: Magnetic Nanoparticles In the Real Worldmentioning

confidence: 99%

Magnetic Nanoparticles: From Design and Synthesis to Real World Applications

et al. 2017

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The increasing number of scientific publications focusing on magnetic materials indicates growing interest in the broader scientific community. Substantial progress was made in the synthesis of magnetic materials of desired size, morphology, chemical composition, and surface chemistry. Physical and chemical stability of magnetic materials is acquired by the coating. Moreover, surface layers of polymers, silica, biomolecules, etc. can be designed to obtain affinity to target molecules. The combination of the ability to respond to the external magnetic field and the rich possibilities of coatings makes magnetic materials universal tool for magnetic separations of small molecules, biomolecules and cells. In the biomedical field, magnetic particles and magnetic composites are utilized as the drug carriers, as contrast agents for magnetic resonance imaging (MRI), and in magnetic hyperthermia. However, the multifunctional magnetic particles enabling the diagnosis and therapy at the same time are emerging. The presented review article summarizes the findings regarding the design and synthesis of magnetic materials focused on biomedical applications. We highlight the utilization of magnetic materials in separation/preconcentration of various molecules and cells, and their use in diagnosis and therapy.

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Section: Magnetic Nanoparticles In the Real Worldmentioning

confidence: 99%

Magnetic Nanoparticles: From Design and Synthesis to Real World Applications

et al. 2017

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“…[29][30][31] Moreover, we found that the formation of particle dimers and small clusters was best observed as a change of the phase lag of the signal, which is insensitive to variations in the MNP concentration. 27 Typical time-resolved optomagnetic spectra are shown in accelerate the recognition rate and lower the detection limit. 32,33 To improve the performance of the proposed NickRCA-based biosensor, magnetic incubation cycles were performed between optomagnetic measurements (see Supplementary Section S2 and Figure S1).…”

Section: Tandem-actingmentioning

confidence: 99%

Ultrasensitive Real-Time Rolling Circle Amplification Detection Enhanced by Nicking-Induced Tandem-Acting Polymerases

Tian

Fock²,

Minero

et al. 2019

Anal. Chem.

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Padlock probe ligation-based rolling circle amplification (RCA) can distinguish singlenucleotide variants, which is promising for the detection of drug-resistance mutations in, e.g., Mycobacterium tuberculosis (Mtb). However, the clinical application of conventional linear RCA is restricted by its unsatisfactory picomolar-level limit of detection (LOD). Herein, we demonstrate the mechanism of a nicking-enhanced RCA (NickRCA) strategy that allows several polymerases to act simultaneously on the same looped template, generating singlestranded amplicon monomers. Limiting factors of NickRCA are investigated and controlled for higher amplification efficiency. Thereafter, we describe a NickRCA-based magnetic nanoparticle (MNP) dimer formation strategy combined with a real-time optomagnetic sensor monitoring MNP dimers. The proposed methodology is applied for the detection of a common Mtb rifampicin-resistance mutation, rpoB 531 (TCG/TTG). Without additional operation steps, an LOD of 15 fM target DNA is achieved with a total assay time of ca. 100 min. Moreover, the proposed biosensor holds the advantages of single-nucleotide mutation discrimination and the robustness to quantify targets in 10% serum samples. NickRCA produces short single-stranded monomers instead of the DNA coils produced in conventional RCA, which makes it more convenient for downstream operation, immobilization or detection, thus being applicable with different molecular tools and biosensors.

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“…The experimental platform of this study was a Poly We have used this affinity-purified CRP antibody because of its well-proven specificity to bind with CRP molecule [21]. CRP-free serum (Hytest Ltd., Turku, Finland) was used for sample dilution and negative control experiments.…”

Section: Materials and Chemicalsmentioning

confidence: 99%

“…In clinical laboratories, the CRP concentration is determined using a latex-enhanced immunoturbidimetric assay [19], [20] on a human serum sample exposed to a number of sample pre-treatment steps. In the literature, different approaches to determine the CRP concentration in human serum have been presented including a magnetic bead-based agglutination assay [21]- [23], quantum dots and immunofiltration [24], quantum dots and immunochromatography [25],…”

Section: Accepted Manuscript Introductionmentioning

confidence: 99%

Combined detection of C-reactive protein and PBMC quantification from whole blood in an integrated lab-on-a-disc microfluidic platform

Uddin

Donolato²,

Hwu

et al. 2018

Sensors and Actuators B: Chemical

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Highlights  A biosensing platform to concurrently detect CRP and PBMC from single blood sample  The microfluidic chip/disc and the readout units are integrated in a single platform  The entire assay procedure is automated from sample to answer  The biosensor needs low sample volume and low sample-to-answer time  It has potential characteristics to be implemented as an out-of-lab setting

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Comparison of optomagnetic and AC susceptibility readouts in a magnetic nanoparticle agglutination assay for detection of C-reactive protein

Cited by 37 publications

References 24 publications

Magnetic Nanoparticles: From Design and Synthesis to Real World Applications

Magnetic Nanoparticles: From Design and Synthesis to Real World Applications

Ultrasensitive Real-Time Rolling Circle Amplification Detection Enhanced by Nicking-Induced Tandem-Acting Polymerases

Combined detection of C-reactive protein and PBMC quantification from whole blood in an integrated lab-on-a-disc microfluidic platform

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