Comparison of ploidy level screening methods in watermelon: Citrullus lanatus (Thunb.) Matsum. and Nakai

Sarı, Nebahat; Abak, K.; Pitrat, Michel

doi:10.1016/s0304-4238(99)00077-1

Cited by 58 publications

(47 citation statements)

References 8 publications

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“…Both direct (chromosome counting) and indirect (flow cytometry, stomatal size, chloroplast number of the guard cells, and morphological observations) methods were used by Sari, Abak, and Pitrat (1999) to determine the most efficient method(s) for ploidy level identification. Their results revealed that all the techniques tested could be successfully used.…”

Section: Watermelon (Citrullus Lanatus)mentioning

confidence: 99%

Cucurbits (Cucurbitaceae; Cucumis spp., Cucurbita spp., Citrullus spp.)

Lebeda¹,

Widrlechner²,

Staub³

et al. 2006

Genetic Resources Chromosome Engineering &Amp; Crop Improvement

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Section: Watermelon (Citrullus Lanatus)mentioning

confidence: 99%

Cucurbits (Cucurbitaceae; Cucumis spp., Cucurbita spp., Citrullus spp.)

Lebeda¹,

Widrlechner²,

Staub³

et al. 2006

Genetic Resources Chromosome Engineering &Amp; Crop Improvement

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“…'embryo maker' 등 표식유전자 (Bingham, 1969;Hermsen and Verdenius, 1973), 기공세포 (Borrino and Powell, 1987), 기공내 엽록체 수 (Chaudhari and Barrow, 1975;Dudley, 1968;Bae et al, 2001), 화분립 (Bamberg and Hanneman, 1991)의 관찰 등이 있으나, 주로 가장 정확한 방법으로 알려진 염색체 수 및 세포내 DNA의 양에 의한 분 석방법이 이용되고 있다 (Galbraith et al, 1983;Miyoshi and Asakura, 1996;Sari et al, 1999) 인용문헌…”

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A Tetraploid Induction in Hypericum patulum Thunberg by Colchicine Soaking Treatment

Kwon¹,

Cho²,

Kim³

2013

Korean Journal of Plant Resources

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-This study aimed to get the basic data on the breeding of good varieties in Hypericum patulum Thunberg. The optimum materials, concentration and soaking time were examined to identify the effective approach to induce tetraploid plant by colchicine treatment to cultivate the varieties. For the seed germination rate of seed by colchicine treatment, the higher colchicine concentration was and the longer soaking time was, the more the germination rate decreased. While individuals were germinated in 16 test groups except control group (no treatment group), all the plants were diploid and no tetraploid was induced. For the plant regeneration rate by colchicine treatment on the explant of Hypericum patulum Thunberg that was under in vitro culture, the higher the colchicine concentration increased, the ress the regeneration rate. While total 147 individuals were regenerated in all treatment, when the explant was soaking treatment in more than 0.05% for over 6 hours, tetraploid could be obtained. In the soaking treatment of 0.05% for over 6 hours, tetraploid could be obtained. In particular, for the soaking treatment in 0.05% for 12 hours, 8 tetraploids were induced, which was about 47.1% of the number of plant regenerated. In accordance with the observation on doubling of DNA contents in leaf in order to identify polyploidy, the peak DNA content of G1 phase was 94.5 for diploid and 192.5 for tetraploid. It confirmed doubling of DNA content. Furthermore, the number of chloroplasts per guard cell depending on polyploid was around 10 in diploid and 17 to 19 in tetraploid, which were around 1.7 to 1.9 times as much as diploid.

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“…Entretanto, como Sari et al (1999) e Souza & Queiróz (2004) afirmam, nem sempre é possível distinguir os diferentes níveis de ploidia com métodos indiretos; assim, dada a influência que o ambiente pode ter em seus resultados, a ploidia deve ser confirmada em etapas posteriores mediante citometria de fluxo ou contagem de cromossomos.…”

Section: Resultsunclassified

Pré-seleção de poliploides por métodos foliares em acessos de bananeira

Jesus

Silva

Silveira

et al. 2016

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar métodos foliares para pré-selecionar poliploides putativos de bananeira em trabalhos com poliploidização in vitro. A espessura foliar, estimada a partir da massa específica das matérias fresca e seca de discos foliares, e a turgescência foliar das plantas, medida diretamente nas plantas às 6h30 da manhã com o aparelho Wiltmeter, foram determinadas em mudas de bananeira diploides (Calcutta e Lidi), triploides (Grande Naine e Williams) e tetraploides (Bucanero e Calypso). A avaliação do conteúdo de DNA foi realizada por citometria de fluxo, tendo-se substituído o diploide Lidi pelo NBA, e o triploide Williams pelo Caipira. Os genótipos com maior ploidia apresentaram maior massa de matéria fresca do disco foliar. O potencial de turgescência foliar também se relacionou significativamente com a ploidia. Os genomas diploides apresentaram os menores conteúdos de DNA (0,96 pg); os triploides Grande Naine (1,48 pg) e Caipira (1,63 pg), conteúdos intermediários; e os tetraploides Calypso (1,90 pg) e Bucanero (2,26 pg), os maiores conteúdos. A massa de matéria fresca de discos foliares e a turgescência foliar das plantas, sob adequado manejo de irrigação, podem ser utilizadas para pré-selecionar tetraploides de bananeira em trabalhos de poliploidização in vitro.Termos para indexação: Musa, citometria de fluxo, conteúdo de DNA, turgescência foliar, Wiltmeter. Preselection of polyploids using leaf methods in banana accessionsAbstract -The objective of this work was to evaluate leaf methods to preselect banana putative polyploids in in vitro works with polyploidization. Leaf thickness, estimated from the specific masses of the fresh and dry matters of leaf disks, and leaf turgor, measured directly on the plants at 6:30 a.m. using the Wiltmeter equipment, were determined in diploid (Calcutta and Lidi), triploid (Grande Naine and Williams), and tetraploid (Bucanero and Calypso) banana. The evaluation of the DNA content was performed by flow cytometry, replacing the diploid Lidi by NBA, and the triploid Williams by Caipira. Genotypes with higher ploidy showed the highest fresh matter mass of the leaf disk. Leaf turgor potential was also significantly related to ploidy. Diploid genomes showed the lowest DNA contents (0.96 pg); the triploids Grande Naine (1.48 pg) and Caipira (1.63 pg), intermediate contents; and the tetraploids Calypso (1.90 pg) and Bucanero (2.26 pg), the highest contents. Fresh matter mass of leaf disks and leaf turgor, under proper irrigation management, can be used to preselect banana tetraploids in in vitro polyploidization studies.Index terms: Musa, flow cytometry, DNA content, leaf turgor, Wiltmeter. IntroduçãoA maioria dos genótipos de bananeira atualmente em uso pela agricultura foi obtida de cruzamentos interespecíficos entre Musa acuminata Colla (genoma A, 2n=2x=22) e M. balbisiana Colla (genoma B, 2n=2x=22). Essas bananeiras são partenocárpicas e estéreis, características que dificultam o melhoramento genético convencional e que, portanto, têm estimulado o...

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Comparison of ploidy level screening methods in watermelon: Citrullus lanatus (Thunb.) Matsum. and Nakai

Cited by 58 publications

References 8 publications

Cucurbits (Cucurbitaceae; Cucumis spp., Cucurbita spp., Citrullus spp.)

Cucurbits (Cucurbitaceae; Cucumis spp., Cucurbita spp., Citrullus spp.)

A Tetraploid Induction in Hypericum patulum Thunberg by Colchicine Soaking Treatment

Pré-seleção de poliploides por métodos foliares em acessos de bananeira

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