Comparison of Quantitative Detection Methods Based on Molecular Fluorescence Spectroscopy and Chromatographic Techniques Used for the Determination of Bisphenol Compounds

Orzel, J.; Świt, Paweł

doi:10.3390/ijms221910569

Cited by 11 publications

(7 citation statements)

References 28 publications

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“…Detection conditions were set based on fluorescent studies [ 26 ] for some of our analytes and our previous works [ 3 , 23 , 27 ]. For three analytes: BPF, BPE and BPA, the detector response is very similar.…”

Section: Resultsmentioning

confidence: 99%

A Fast Method for Determination of Seven Bisphenols in Human Breast Milk Samples with the Use of HPLC-FLD

Szubartowski

Tuzimski

2023

Molecules

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Plastic pollution, where bisphenol A (BPA) is widely used in its production, has gained popularity. BPA omnipresence and toxicity, especially for infants, has led food safety authorities to place restrictions on BPA usage. It has led to the introduction of the marked ‘BPA-free’-labelled products, where BPA is often replaced by other bisphenols (BPs) which are suspected of being similar or even more toxic than BPA. Moreover, the free forms of BPs are more dangerous than their conjugated forms and the conjugation of BPs is less effective in infants than in adults. Considering that human breast milk is the main source of nutrition for infants, the constant biomonitoring not only of BPA, but the wider group of BPs in such crucial matrices seems to be vital. In this study, a fast, simple, ‘green’ and cost-effective DLLME-based extraction technique combined with HPLC-FLD was optimized for the determination of seven selected bisphenols simultaneously. The procedure has satisfactory recovery values of 67–110% with the most RSD% at 17%. The LODs and LOQs ranged from 0.5 ng/mL to 2.1 ng/mL and 1.4 ng/mL to 6.3 ng/mL, respectively. The procedure was successfully applied to the biomonitoring of free forms of BPs in 10 real human breast milk samples.

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Section: Resultsmentioning

confidence: 99%

A Fast Method for Determination of Seven Bisphenols in Human Breast Milk Samples with the Use of HPLC-FLD

Szubartowski

Tuzimski

2023

Molecules

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“…The FLD enabled analysis across four different optimal wavelengths simultaneously, making it possible to obtain four different chromatograms and choose the most optimal one. Some bisphenols are described as a fluorescent molecules [34]. The bisphenols exhibited different fluorescent properties.…”

Section: Detection Conditionsmentioning

confidence: 99%

“…The detector responses for BPF and BPE were similar, and their structures differed only in the numbers of -CH 3 substituents attached to the carbon atom connecting the two phenyl rings. The FLD detector settings, such as excitation wavelength and emission wavelength, were selected based on a study of the fluorescence properties of BPA, BPS, and BPF [34]. Then, thanks to the possibility of measuring at four different excitation wavelengths with the FLD, four excitation wavelengths were selected (225, 230, 235, and 240 nm), while the emission wavelength was set to 300 nm during all analyses.…”

Section: Detection Conditionsmentioning

confidence: 99%

Application of High-Performance Liquid Chromatography Combined with Fluorescence Detector and Dispersive Liquid–Liquid Microextraction to Quantification of Selected Bisphenols in Human Amniotic Fluid Samples

Szubartowski

Tuzimski

2022

IJERPH

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Bisphenol A (BPA) is a widely produced chemical worldwide found in numerous everyday products. Its endocrine-disrupting properties and omnipresence have aroused concern and led to several restrictions on its use. These restrictions and growing public awareness about the toxicity of BPA have resulted in market products labeled ”BPA-free”, with BPAs often being replaced by other bisphenols. This is why constant biomonitoring of bisphenol levels in various body fluids and tissues is essential. In this study, we propose the use of simple, cost-effective high-performance liquid chromatography coupled with the fluorescence detector (HPLC-FLD) method for the determination of simultaneously selected bisphenols in amniotic fluid. For the sample preparation, a fast, simple, and ”green” dispersive liquid–liquid microextraction (DLLME) method was used, achieving mean recovery values in the range of 80.9–115.9% with relative standard deviations below 12% for all analytes. Limits of quantification (LOQs) determined in the amniotic fluid matrix ranged from 6.17 to 22.72 ng/mL and were obtained from a calibration curve constructed using least-squares linear regression analysis for all cases. The presented sample preparation procedure can be easily adopted for LC-MS analysis.

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“…Orzel and Swit [31] compared quantitative detection methods based on molecular fluorescence spectroscopy and chromatographic techniques used for the determination of bisphenol compounds and concluded that high performance liquid chromatography with fluorescence detector (HPLC-FLD) result in the highest sensitivity and the lowest limit of detection (LODs) values. Due to similar spectral properties of BPA and BPF, simultaneous detection using the fluorescence approach is impossible [31]. This work supports our previous decision to use HPLC-FLD for the qualification and quantification of BPA migration from different packaging items and household products.…”

Section: Introductionmentioning

confidence: 99%

Migration of BPA from Food Packaging and Household Products on the Croatian Market

Krivohlavek

Mikulec

Budeč

et al. 2023

IJERPH

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BPA is a plasticizer for the production of polycarbonate plastics and epoxy resins and is widely used in the production of household goods, including food packaging. Free BPA is known to migrate from packaging to food, and its uptake has been associated with adverse health effect, particularly the disruption of endocrine activity. The presence and migration of BPA from plastic consumer products are subject to strict regulation in the EU. The aim of this study is to analyse the migration of BPA from different packaging items and household products sold on the Croatian market. To simulate real life exposure, we treated samples with a food simulant. The analytical performance was confirmed with the EU requirements. BPA levels were assessed in 61 samples by HPLC-FLD and the LOQ of the method was 0.005 mg kg−1 for the food simulant. These results showed that the levels of BPA that migrated to the food simulant were below LOQ and in accordance with the specific migration limit into food, which was defined as 0.05 mg kg−1 for all samples. None of the analysed products presented a health hazard. However, these regulations do not refer to products intended for children’s use, in which BPA is banned. Furthermore, regulations require testing before putting products on the market, and previous research shows that possible BPA migration occurs due to various uses, along with a cumulative effect of exposure from even very small concentrations. Therefore, for accurate BPA consumer exposure evaluation and possible health risks, a comprehensive approach is needed.

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Comparison of Quantitative Detection Methods Based on Molecular Fluorescence Spectroscopy and Chromatographic Techniques Used for the Determination of Bisphenol Compounds

Cited by 11 publications

References 28 publications

A Fast Method for Determination of Seven Bisphenols in Human Breast Milk Samples with the Use of HPLC-FLD

A Fast Method for Determination of Seven Bisphenols in Human Breast Milk Samples with the Use of HPLC-FLD

Application of High-Performance Liquid Chromatography Combined with Fluorescence Detector and Dispersive Liquid–Liquid Microextraction to Quantification of Selected Bisphenols in Human Amniotic Fluid Samples

Migration of BPA from Food Packaging and Household Products on the Croatian Market

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