Comparison of rat and human alkaline phosphatase isoenzymes and isoforms using HPLC and electrophoresis

Dziedziejko, Violetta; Safranow, Krzysztof; Słowik-Zyłka, Dorota; Machoy-Mokrzyńska, Anna; Millo, Barbara; Machoy, Z; Chlubek, Dariusz

doi:10.1016/j.bbapap.2005.05.013

Cited by 16 publications

(4 citation statements)

References 34 publications

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“…This information is required to more clearly delineate the respective roles of IAP and other AP isoforms. Combining chromatography, electrophoresis, enzymology, and immunochemistry will help resolve this complexity 102–104 …”

Section: Resultsmentioning

confidence: 99%

Intestinal alkaline phosphatase: multiple biological roles in maintenance of intestinal homeostasis and modulation by diet

Lallès¹

2010

Nutrition Reviews

314

284

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The diverse nature of intestinal alkaline phosphatase (IAP) functions has remained elusive, and it is only recently that four additional major functions of IAP have been revealed. The present review analyzes the earlier literature on the dietary factors modulating IAP activity in light of these new findings. IAP regulates lipid absorption across the apical membrane of enterocytes, participates in the regulation of bicarbonate secretion and of duodenal surface pH, limits bacterial transepithelial passage, and finally controls bacterial endotoxin-induced inflammation by dephosphorylation, thus detoxifying intestinal lipopolysaccharide. Many dietary components, including fat, protein, and carbohydrate, modulate IAP expression or activity and may be combined to sustain a high level of IAP activity. In conclusion, IAP has a pivotal role in intestinal homeostasis and its activity could be increased through the diet. This is especially true in pathological situations (e.g., inflammatory bowel diseases) in which the involvement of commensal bacteria is suspected and when intestinal AP is too low to detoxify a sufficient amount of bacterial lipopolysaccharide.

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Section: Resultsmentioning

confidence: 99%

Intestinal alkaline phosphatase: multiple biological roles in maintenance of intestinal homeostasis and modulation by diet

Lallès¹

2010

Nutrition Reviews

314

284

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“…Az ALP egy glikoprotein, amely a sejtmembrán külső részéhez glikozil-foszfatidil-inozitol karral kapcsolódik [1].…”

Section: áBraunclassified

The role of intestinal alkaline phosphatase in pediatric inflammatory bowel and celiac diseases

et al. 2012

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Az intestinalis alkalikus foszfatáz enzim alapvető fontosságú a bélnyálkahártya épségének fenntartásában, mivel detoxifi kálni képes a lipopoliszacharidot, amely a Toll-like receptor-4 ligandja. A helytelen immunválasz, valamint a mucosalis barrier sérülése hozzájárulhat a gyulladásos bélbetegség és a coeliakia kialakulásához. Gyulladásos bél-betegségben szenvedő gyermekek gyulladt vastagbél-és újonnan diagnosztizált coeliakiás gyermekek duodenumnyálkahártyájában a csökkent intestinalis alkalikus foszfatáz és a Toll-like receptor-4 fehérjeexpresszió emelkedése következményesen fokozott lipopoliszacharid-aktivitással járhat, amely a szövetkárosító folyamatokat erősítheti. Az intestinalis alkalikus foszfatáz aktivitásának elősegítése colitises állatmodellben és terápiarezisztens colitis ulcerosás felnőtt betegekben csökkentette a bélgyulladás tüneteit. Ennek megfelelően a két vizsgált kórképben az enzim célzott intestinalis bejuttatása kiegészítő terápiás konzekvenciával is járhat a jövőben. Orv. Hetil., 2012Hetil., , 153, 1389Hetil., -1395

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“…While commonly used for the basic separation and visualization of enzymes and biomolecules in research, it may not be ideal for high-throughput or clinical applications. For the separation and analysis of ALP isoenzymes, conventional electrophoresis techniques such as affinity electrophoresis with wheatgerm lectin [10,11] and isoelectric focusing in immobilized pH gradients are commonly employed [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Overview of Capillary Electrophoresis Analysis of Alkaline Phosphatase (ALP) with Emphasis on Post-Translational Modifications (PTMs)

Balbaied,

Moore

2023

Kinases and Phosphatases

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Alkaline phosphatase is a vital enzyme used in separation studies and as a biomarker for liver, bone, and certain cancer conditions. Its stability and specific properties enable insights into enzyme behavior, aiding in the development of detection methods with broader applications in various scientific fields. Alkaline phosphatase has four main isoenzymes: GCAP, IAP, PLAP, and TNAP, each with distinct roles. TNAP is found in the liver, kidney, and bones, playing a role in bone mineralization. The functions of the other isoenzymes are not fully known. Separation techniques like electrophoresis and chromatography are valuable for studying enzymes and proteins, revealing insights into their structure and function in pharmaceutical research and PTM studies. The main goal of this review paper is to thoroughly evaluate how capillary electrophoresis is applied to analyze alkaline phosphatase. It seeks to investigate the latest advancements in capillary electrophoresis and how they can improve the sensitivity, selectivity, and efficiency of alkaline phosphatase analysis.

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Comparison of rat and human alkaline phosphatase isoenzymes and isoforms using HPLC and electrophoresis

Cited by 16 publications

References 34 publications

Intestinal alkaline phosphatase: multiple biological roles in maintenance of intestinal homeostasis and modulation by diet

Intestinal alkaline phosphatase: multiple biological roles in maintenance of intestinal homeostasis and modulation by diet

The role of intestinal alkaline phosphatase in pediatric inflammatory bowel and celiac diseases

Overview of Capillary Electrophoresis Analysis of Alkaline Phosphatase (ALP) with Emphasis on Post-Translational Modifications (PTMs)

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