Comparison of stem cell properties of cells isolated from normal and inflamed dental pulps

Pereira, Luciana Oliveira; Rubini, Marciano Régis; Silva, Jaqueline R.; Oliveira, Dutra de; Silva, I. C. R.; Poças-Fonseca, Marcio José; Azevedo, Ricardo Bentes

doi:10.1111/j.1365-2591.2012.02068.x

Cited by 65 publications

(50 citation statements)

References 31 publications

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“…STRO-1 has been previously observed in pulp tissues and is regarded as a marker for stromal stem cells, recognizing the trypsin-insensitive epitope on perivascular cells (4). In the majority of previous studies, STRO-1 expression has been observed in DPSCs, with an expression level ranging between 0.46 and 26.14% (4,14,17). CD146 is a stem cell marker identified in adult bone marrow and dental pulp tissue, associated with blood vessels (18).…”

Section: Discussionmentioning

confidence: 99%

Odontogenic differentiation of vascular endothelial growth factor-transfected human dental pulp stem cells in vitro

Zhang

Liu

Zhao

et al. 2014

Molecular Medicine Reports

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Section: Discussionmentioning

confidence: 99%

Odontogenic differentiation of vascular endothelial growth factor-transfected human dental pulp stem cells in vitro

Zhang

Liu

Zhao

et al. 2014

Molecular Medicine Reports

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“…This ablative approach is based on the generally accepted belief that once infection reaches the pulp, the infected and locally inflamed neurovascular structure may be irreversibly damaged with limited capability for repair. Recent studies reported that a subset of MSCs derived from inflamed human pulps preserve the full capability of proliferation and multipotent differentiation as compared with those from healthy pulps (DPSCs; Alongi et al 2010;Wang et al 2010;Pereira et al 2012), suggesting that inflamed pulp may not be completely depleted of progenitor/stem cells but can be repaired.…”

Section: Introductionmentioning

confidence: 99%

DPSCs from Inflamed Pulp Modulate Macrophage Function via the TNF-α/IDO Axis

et al. 2016

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Human dental pulp stem cells (DPSCs) can be isolated from inflamed pulp derived from carious teeth with symptomatic irreversible pulpitis (I-DPSCs), which possess stemness and multidifferentiation potentials similar to DPSCs from healthy pulp. Since macrophagesessential cell players of the pulpal innate immunity-can regulate pulpal inflammation and repair, the authors investigated the immunomodulatory effects of DPSCs/I-DPSCs on macrophage functions and their underlying mechanisms. Similar to DPSCs, I-DPSCs were capable of colony-forming efficiency and adipogenic and osteo/dentinogenic differentiation under in vitro induction conditions. I-DPSCs also expressed a similar phenotypic profile of mesenchymal stem cell markers, except a relatively higher level of CD146 as compared with DPSCs. Coculture of DPSCs or I-DPSCs with differentiated THP-1 cells, the human monocyte cell line, markedly suppressed tumor necrosis factor α (TNF-α) secretion in response to stimulation with lipopolysaccharides (LPS) and/or nigericin. However, unlike TNF-α, the secreted level of interleukin 1β was not affected by coculture with DPSCs or I-DPSCs. Furthermore, DPSC/I-DPSC-mediated inhibition of TNF-α secretion by macrophages was abolished by pretreatment with 1-methyl-D-tryptophan, a specific inhibitor of indoleamine-pyrrole 2,3-dioxygenase (IDO), but not by NSC-398, a specific inhibitor of COX-2, suggesting IDO as a mediator. Interestingly, IDO expression was significantly augmented in macrophages and mesenchymal stromal cells in inflamed human pulp tissues. Collectively, these findings show that I-DPSCs, similar to DPSCs, possess stem cell properties and suppress macrophage functions via the TNF-α/IDO axis, thereby providing a physiologically relevant context for their innate immunomodulatory activity in the dental pulp and their capability for pulp repair.

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“…Recent studies have compared the cells from normal and inflamed human dental pulps regarding the presence of stem cells, their proliferation and differentiation potentials [27][28][29][30]. Diseased pulp contains viable cells with the potential for ex vivo expansion and proliferation, a similar percentage of STRO-1-positive cells and ex vivo odonto-osteogenic differentiation capacity.…”

Section: Dentin-pulp Tissue Regeneration With Inflamed Pulp Tissuementioning

confidence: 99%

Dental Stem Cells: Sources and Potential Applications

Lee

Zhang

2014

Curr Oral Health Rep

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Comparison of stem cell properties of cells isolated from normal and inflamed dental pulps

Abstract: The morphology, proliferation rate and differentiation potential of DPSC-I were similar to the observed in DPSC-N, thus demonstrating that the inflammatory process did not affect the stem cell properties that were assessed.

Cited by 65 publications

References 31 publications

Odontogenic differentiation of vascular endothelial growth factor-transfected human dental pulp stem cells in vitro

Odontogenic differentiation of vascular endothelial growth factor-transfected human dental pulp stem cells in vitro

DPSCs from Inflamed Pulp Modulate Macrophage Function via the TNF-α/IDO Axis

Dental Stem Cells: Sources and Potential Applications

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