Comparison of structural and antioxidant activity of polysaccharide extracted from truffles

Abstract

As the main component of truffles, polysaccharides have a variety of biological activities such as anti-oxidation, anti-tumor, and hypoglycemic activity, and these activities are closely related to its structure. In this study, Tuber Aestivum crude polysaccharide (TACP) and Tuber Melanosporum crude polysaccharide (TMCP) were obtained from Tuber Aestivum and Tuber Melanosporum by using microwave-assisted hot water, and then the Sephadex G-200 column was utilized to further separate and purify Tuber Aestivum polysaccharide (TAP) and Tuber Melanosporum polysaccharide (TMP) from TACP and TMCP. The structural characterization results showed that the molecular weight of TAP was 2.18 × 10 4 kDa, while TMP was 8.79 × 10 3 kDa. Although the two polysaccharide components were mainly composed of mannose (Man) and glucose (Glc), the molar ratio of Man and Glc in TAP was 14.76: 12.31, with a molar ratio of 5.43:10.94 in TMP. Furthermore, the antioxidant activity of two polysaccharide components was evaluated. TAP and TMP could protect porcine jejunal epithelial (IPEC-J2) cells from oxidative damage by H 2 O 2 , but TAP exhibited stronger antioxidant effects. It was mainly reflected that TAP could increase the secretion level of intracellular antioxidant enzymes (superoxide dismutase and catalase) in IPEC-J2 cells, and had a significant effect on the total antioxidant capacity of cells. The reactive oxygen species and malondialdehyde had better scavenging ability at the concentration of 20 µg/ml. The difference between TAP and TMP may be due to the dissimilar structure. Its structure-activity relationship needs further study. K E Y W O R D Santioxidant activity, structural characterization, Tuber aestivum polysaccharide, Tuber melanosporum polysaccharide Practical Application: The structure of TAP and TMP were different, and TAP had higher molecular weight. Besides, TAP and TMP can protect IPEC-J2 cells from oxidative stress, providing a theoretical basis for developing potential antioxidant drugs of practical significance.

Effect of Flammulina velutipes polysaccharides on endoplasmic reticulum stress‐mediated apoptosis by activating PLC–IP3 pathway in HepG2 cells

“…At 1077.78018 cm −1 was the β-pyranose ring in the glucose residue. The results indicated that FVP might be β-pyran polysaccharides (Guan et al, 2022;Zhang et al, 2018) (Figure S1d).…”

Structural characterization of three acidic polysaccharides from Opuntia dillenii Haw. fruits and their protective effect against hydrogen peroxide-induced oxidative stress in Huh-7 cells

“…The results suggested that OFPPs exhibited protective effect on Huh-7 cells against H 2 O 2 -induced oxidative stress and increased the viability of the cells. Polysaccharide extracted from truffles has also been reported to enhance the viability of the H 2 O 2 -induced IPC-J2 cells and protect the cells from oxidative stress [54] . The anti-oxidative stress activity of OFPPs can be further explored by determining the level of intracellular oxidative stress.…”

High-Molecular-Weight Exopolysaccharides Production from Tuber borchii Cultivated by Submerged Fermentation

“…Polysaccharides with low MW have the potential to penetrate into immune cells and generate an immune response [20]. However, the antioxidant property of a polysaccharide is inversely associated with its MW, i.e., polysaccharides with lower molecular exhibit greater antioxidant activity, whereas high-MW polysaccharides are thought to have potent immunomodulatory and anti-tumor activities [20][21][22].…”