Comparison of techniques for primary isolation of respiratory Mycoplasma pulmonis from rats

Abstract

Studies were performed to determine the optimal sites for primary isolation of Mycoplasma pulmonis from the respiratory system of laboratory rats. In a preliminary study, a group of 42 rats was cultured for respiratory M. pulnonis at several sites. Tracheal washes yielded the highest number of positive cultures (61.9%) and ground lung tissue yielded the lowest (19.0%), with the nasopharyngeal culture in the middle (52.38%). In a subsequent study, the tracheal wash was again the optimal method since 76.7% of 103 rats were positive for M. pulmonis, whereas culture of the nasopharyngeal area resulted in 53.9% positive cultures. In a third study, all 45 rats were positive for M. pubmonis when the tracheal wash method was used, whereas only 51% were positive when the nasopharyngeal area was cultured, indicating that the tracheal wash was the most reliable of the commonly used procedures. Other aspects of these experiments demonstrated the tympanic cavity of animals with otitis media to be an excellent source for obtaining material to culture for M. pulmonis.

Detection of Mycoplasma pulmonis in Laboratory Rats and Technicians

“…The tracheal specimen of rats for isolating M. pulmonis were chosen because this procedure is highly recommended for isolating this mycoplasma from this host (Lentsch et al., 1979; Davidson et al., 1981; Barreto et al., 2002). Isolation of M. pulmonis from rats may be prejudiced by viral and bacterial infections (Davidson et al., 1981).…”

Comparison of Chalquest and Hayflick media, with and without ammonium reineckate, for isolating Mycoplasma pulmonis from rats

“…Chalquest agar medium modified as described previously (8,14) was prepared as follows: 10.5 g of PPLO broth without crystal violet, 5.8 g of special agar Noble, 2.5 g of soluble starch, and 2.5 g of glucose were added to 450 ml of deionized water, and the mixture was boiled briefly. Five a A total of 198 samples were taken from 66 rats.…”

“…Hayflick's original formula thereof are used frequently to isolate M. pulmonis from -ir isolation is rodents, although diagnostic laboratories that support labonposition and ratory animal resources are turning to other formulations d characteris-which better support the growth of rodent mycoplasmas. re media that Chalquest medium (3) or variations (8,14) have been used presence or with increasing frequency in laboratory animal medicine but d rats. have never been adequately compared with other media. mycoplasmas…”

Detecção de Mycoplasma pulmonis e Mycoplasma arthritidis no trato respiratório superior de ratos e bioteristas por cultivo e reação em cadeia da polimerase (PCR)

“…Neste estudo optou-se por realizar a coleta de material clínico utilizando-se a técnica de lavagem traqueal, devido ao alto grau de isolamento de M. pulmonis quando comparados a outros sítios anatômicos (LENTSCH et al, 1979;LAI et al, 1986;BARRETO et al, 2002). Taylor-Robinson et al (1972) Sanchez et al (1994) e Goto et al (1994) e similar aos resultados obtidos por outros pesquisadores (LAI et al, 1986;SIMECKA et al, 1992).…”

“…Davidson et al (1981) isolaram 71% (155/219) de M. pulmonis em meio líquido e 47% (102/219) em meio sólido. Lentsch et al (1979) obtiveram o isolamento desta espécie em 76,7% (79/103) dos lavados traqueais realizados. Machii et al (1985) apresentaram a positividade de 78% (58/74).…”