Comparison of TEST 1 with SRS 100 and ICSH reference method for the measurement of the length of sedimentation reaction in blood

Özdem, Sebahat; Akbaş, Halide; Dönmez, Levent; Gültekin, Meral

doi:10.1515/cclm.2006.075

Cited by 22 publications

(22 citation statements)

References 15 publications

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“…8 Some of them have the advantage of being automated or semi-automated, having shorter testing times, and/or reducing biohazard exposure during testing as the samples are aspirated from closed tubes. 2,5,7,10,[15][16][17][18][19][20] These alternate methods adopt procedures for measuring rouleaux formation that is distinct from the Westergren or the modified Westergren method, such as centrifugation or photometric rheology. Some studies have shown that the ESR measured by the microhemagglutination method better reflects inflammation than that measured by the Westergren method in malignancies, autoimmune diseases, or infections.…”

Section: Several New Methods For Esr Measurement Became Availablementioning

confidence: 99%

Erythrocyte sedimentation rate measured using microhemagglutination is not elevated in monoclonal gammopathy compared with other diseases

Kim

Lee

et al. 2018

Int J Lab Hematology

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Section: Several New Methods For Esr Measurement Became Availablementioning

confidence: 99%

Erythrocyte sedimentation rate measured using microhemagglutination is not elevated in monoclonal gammopathy compared with other diseases

Kim

Lee

et al. 2018

Int J Lab Hematology

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“…Very few of these publications used the reference method with undiluted EDTA samples (Caswell & Stuart, 1991; Plebani et al. , 1998; Ozdem et al. , 2006) and did not apply the comparison table included in ICSH‐1993.…”

Section: Review Of the Literature: Use Of The Esr Reference And Standmentioning

confidence: 99%

“…, 2001; Happe et al. , 2002; Al‐Fadhli & Al‐Awadhi, 2005; Ozdem et al. , 2006; Osei‐Bimpong, Meek & Lewis, 2007; Mahlangu & Davids, 2008; Shelat, Chacosky & Shibutani, 2008; Alexy, Pais & Meiselman, 2009; Perovic, Bakovic & Valcic, 2010).…”

Section: Evaluation Of New Analysersmentioning

confidence: 99%

ICSH review of the measurement of the erythocyte sedimentation rate

Jou¹,

Lewis²,

Briggs³

et al. 2011

International Journal of Laboratory Hematology

120

130

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In recognition of the need for a standardization of the measurement of the erythrocyte sedimentation rate (ESR), the International Council for Standardization in Haematology makes the following recommendations: (i) The reference method for measurement of the ESR should be based on the Westergren method, which is a specific test for the ESR, with modifications, (ii) The reference method for measurement of the ESR should use either whole blood anticoagulated with EDTA and later diluted with sodium citrate or saline (4 : 1) or whole blood anticoagulated with sodium citrate (4 : 1) in Westergren pipettes, (iii) The ESR pipettes can be of glass or plastic (with specific characteristics). It must be colourless; a minimum sedimentation scale of 200 mm, a minimum bore of 2.55 mm, which should be constant within 5%. A protocol for the evaluation of alternative methodologies against the reference method is outlined: The new technologies must be tested over a range of ESR values of 2-120 mm. In this comparison, 95% of the differences should be 5 mm or less, with larger differences associated with higher ESR values. A minimum of 40 samples should be tested in 3 different groups of values: 1-20, 21-60 and more than 60 mm. The statistical methods recommended for ESR evaluations are the coefficient of correlation, the Passing-Bablock regression and the Bland-Altman statistical method. This reference method replaces all earlier standardized and reference methods.

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“…It is important that while making comparison between Alifax Roller 20LC and standardized method the nature of sample-diluted or undiluted is kept in mind. As most of the automated analyzer use undiluted sample whereas standardized methods can use either diluted or undiluted samples, while comparing two results sample harmony and matrix has to be similar else the conclusion will be invalid [13,14,15] . The newer technologies tend to use undiluted EDTA samples for making them user friendly without the need of transferring samples from EDTA vials to ESR vial for making them user friendly and economical.…”

Section: Discussionmentioning

confidence: 99%

Comparison of erythrocyte sedimentation rate by Alifax Roller 20 LC method and standard Westergreen method

Sareen¹,

Kapil²,

Govil³

et al. 2019

Int. J. Clin. Diagn. Pathol.

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Erythrocyte sedimentation rate (ESR) is the most common test done for diagnosis and follow up treatment in patients suffering from autoimmune disorders and chronic illness. It is imperative for clinicians to understand the methodology, limitations and variations in testing platforms for ESR from ICSH standardized Westergreen method to various automated testing techniques. We conducted a retrospective study of 150 patients who were tested for ESR using Westergreen method and new automated Alifax Roller 20LC. The data was analyzed by applying Pearsons coefficient of correlation and Bland Altman analysis. It was observed that the automated Alifax Roller 20LC system tends to estimate ESR with positive bias. The maximum agreement between two methods was observed for ESR values between 20-40 mm/hr. As the likelihood of two similar results between two methods is uncertain, the difference between values should be acceptable clinically before replacing the ICSH Westergreen method with any new automated system.

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Comparison of TEST 1 with SRS 100 and ICSH reference method for the measurement of the length of sedimentation reaction in blood

Abstract: The findings suggested that TEST 1 is a reliable, precise and accurate system for measurement of LSRB in clinical laboratories with high workload.

Cited by 22 publications

References 15 publications

Erythrocyte sedimentation rate measured using microhemagglutination is not elevated in monoclonal gammopathy compared with other diseases

Erythrocyte sedimentation rate measured using microhemagglutination is not elevated in monoclonal gammopathy compared with other diseases

ICSH review of the measurement of the erythocyte sedimentation rate

Comparison of erythrocyte sedimentation rate by Alifax Roller 20 LC method and standard Westergreen method

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