Comparison of the Allatostatin Neuropeptide Precursors in the Distantly Related Cockroaches

Ding, Qi; Donly, B. Cameron; Tobe, Stephen S.; Bendena, William G.

doi:10.1111/j.1432-1033.1995.737_a.x

Cited by 73 publications

(34 citation statements)

References 48 publications

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“…Tt seems highly likely that the acidic sequence is a part of one of the spacer regions, rich in Glu and Asp residues and characteristic of the allatostatin genes, that in this instance has not been cleaved. Precisely the same -EDEDED-sequence occurs in the allatostatin gene of P americunu (Ding et al, 1995). The -YALGL-sequence (followed by the sequence containing a putative cleavage point and an amide donor, -GKR) is a probable corruption of the normal -YAFGLthat occurred as a mutation at some point during the evolution of this allatostatin gene.…”

Section: Discussionmentioning

confidence: 99%

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Isolation and Identification of Multiple Neuropeptides of the Allatostatin Superfamily in the Shore Crab Carcinus Maenas

Duve

Johnsen

Maestro

et al. 1997

European Journal of Biochemistry

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20 neuropeptides belonging to the allatostatin superfamily were isolated from extracts of cerebral and thoracic ganglia of the shore crab Carcinus maenas. They were purified by HPLC, monitored by radioimmunoassay and identified by mass spectrometry and amino acid sequencing. The allatostatins are characterised by a common C‐terminal pentapeptide sequence ‐YXFGL‐NH2. Previously such peptides have only been reported from insects. In insects the variable post‐tyrosyl residue is restricted to Ala, Asn, Asp, Gly or Ser. In C. maenas, however, there are only two types; thirteen of the peptides having a post‐tyrosyl Ala and the other seven, a post‐tyrosyl Ser. The crab peptides include the shortest allatostatins so far identified (YAFGL‐NH2 and YSFGL‐NH2) as well as the longest, a 27‐residue peptide. The total of 20 peptides exceeds the highest number of allatostatins found in any of the insects investigated so far (14 in Periplaneta americana). It is of interest that, despite their clear homology, none of the peptides of C. maenas is identical to any of the more than 50 known insect allatostatins. The crab allatostatins show evidence of gene duplication and mutation that has resulted in several sub‐groups with close structural similarities. For example, there are four heptapeptides with the common C‐terminus ‐PYAFGL‐NH2 that differ only at the N‐terminal residue, which is either Glu, Asp, Asn or Ser. Other motifs, variously extended at the N‐terminus, include ‐GPY(A/S)FGL‐NH2 (three peptides), ‐DMY(A/S)FGL‐NH2 (three peptides), and ‐GQY(A/S)FGL‐NH2 (two peptides). Unique among the allatostatin superfamily, one of the crab peptides has a Tyr for Phe substitution at position three from the C‐terminus (GGPYSYGL‐NH2). Immunocytochemistry has provided clues to the functions of the allatostatins in crustaceans by showing their widespread presence in the central and stomatogastric nervous systems.

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Section: Discussionmentioning

confidence: 99%

“…In addition to peptide isolation, the allatostatin genes of several insects have been cloned and sequenced (Donly et al, 1993 ;Ding et al, 1995;East et al, 1996;Vanden Broek et al, 1996). The peptides identified within this superfamily are listed in Fig.…”

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confidence: 99%

Isolation and Identification of Multiple Neuropeptides of the Allatostatin Superfamily in the Shore Crab Carcinus Maenas

Duve

Johnsen

Maestro

et al. 1997

European Journal of Biochemistry

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“…In cockroaches, precursors encoding 13 (Diploptera) or 14 (Leucophea) allatostatins were discovered. 33 In locusts (Schistocerca gregaria), a precursor encoding 10 allatostatins was cloned. 34 In Drosophila, allatostatin-like neuropeptides have been localized in the CNS and the gut.…”

Section: The Allatostatin Precursormentioning

confidence: 99%

Peptidomic analysis of the larval Drosophila melanogaster central nervous system by two‐dimensional capillary liquid chromatography quadrupole time‐of‐flight mass spectrometry

et al. 2005

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Peptides are the largest class of signalling molecules found in animals. Nevertheless, in most proteomic studies peptides are overlooked since they literally fall through the mazes of the net. In analogy with proteomics technology, where all proteins expressed in a cell or tissue are analyzed, the peptidomic approach aims at the simultaneous visualization and identification of the whole peptidome of a cell or tissue, i.e. all expressed peptides with their post-translational modifications. In this paper we describe the analysis of the larval fruit fly central nervous system using two-dimensional capillary liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (LC/Q-TOF-MS/MS. Using the central nervous systems of only 50 larval Drosophila as starting material, we identified 38 peptides in a single analysis, 20 of which were not detected in a previous study that reported on the one-dimensional capillary LC/MS/MS analysis of the same tissue. Among the 38 sequenced peptides, some originate from precursors, such as the tachykinin and the IFamide precursor that were entirely missed in the first study. This clearly demonstrates that the two-dimensional capillary LC approach enhances the coverage of the peptidomic analysis.

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“…The allatostatins (ASTs) consist of a family of peptides present in various insects including cockroaches (Ding et al, 1995;Vilaplana et al, 1999), moths (Audsley and Weaver, 2003;Kramer et al, 1991), locusts (Skiebe et al, 2006;Veelaert et al, 1996a;Veelaert et al, 1996b), mosquitoes (HernandezMartinez et al, 2005) and flies (Duve et al, 1993;Lenz et al, 2001;Williamson et al, 2001). In crustaceans, including C. borealis, AST-like immunoreactivity has been detected in the POs and other structures (Christie et al, 1995;Kilman et al, 1999;Pulver and Marder, 2002;Skiebe, 1999;Skiebe, 2001;Skiebe and Schneider, 1994;Yasuda-Kamatani and Yasuda, 2006).…”

Section: Introductionmentioning

confidence: 99%

Multiple modulators act on the cardiac ganglion of the crab,Cancer borealis

Cruz-Bermúdez

Marder

2007

Journal of Experimental Biology

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crustacean cardioactive peptide (CCAP), a number of extended FLRFamide peptides, and cholinergic agonists increased the activity of the CG, GABA inhibited the CG, while other substances had little or no significant effect on the CG motor pattern. These results demonstrate, in one species, that the CG is multiply modulated. We suggest that multiple modulators may be important to regulate and coordinate the activity of the heart and other organs in response to external stimuli or the endogenous physiological state.

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Comparison of the Allatostatin Neuropeptide Precursors in the Distantly Related Cockroaches

Cited by 73 publications

References 48 publications

Isolation and Identification of Multiple Neuropeptides of the Allatostatin Superfamily in the Shore Crab Carcinus Maenas

Isolation and Identification of Multiple Neuropeptides of the Allatostatin Superfamily in the Shore Crab Carcinus Maenas

Peptidomic analysis of the larval Drosophila melanogaster central nervous system by two‐dimensional capillary liquid chromatography quadrupole time‐of‐flight mass spectrometry

Multiple modulators act on the cardiac ganglion of the crab,Cancer borealis

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