Comparison of the in Vitro Susceptibility of Rodent Isolates of Pseudomonas Aeruginosa and Pasteurella Pneumotropica to Enrofloxacin

Abstract

Abstract. The objectives of this study were to determine and compare the in vitro enrofloxacin susceptibility of 94 Pseudomonas aeruginosa isolates obtained from enrofloxacin-treated and untreated mice and that of 40 Pasteurella pneumotropica strains and also to assess the efficacy and effects of enrofloxacin treatment of laboratory mice. The minimum inhibitory concentrations (MICs) of enrofloxacin against all the Ps. aeruginosa isolates were in the range of 1 to 4 mg/ml, whereas those against all the P. pneumotropica strains were less than 0.5 mg/ml. The mutation frequency in 54% of the Ps. aeruginosa isolates on treatment with enrofloxacin ranged from 10 26 to 10 28 ; however, none of the P. pneumotropica strains could grow on medium containing more than 3 mg/ml enrofloxacin. Comparison of in vitro enrofloxacin susceptibilities suggested that enrofloxacin was effective for eliminating P. pneumotropica but not for eliminating Ps. aeruginosa for which the MIC of enrofloxacin was more than 1 mg/ml. These results indicated that the enrofloxacin susceptibility of P. pneumotropica was higher than that of Ps. aeruginosa, and that the enrofloxacin treatment might not affect the susceptibility of Ps. aeruginosa.

Biology and Diseases of Mice

“…Treatment Antibiotic sensitivity testing in vitro indicated P. pneumotropica was significantly more sensitive than P. aeruginosa to enrofloxacin (Sasaki et al, 2007). Enrofloxacin in the drinking water at 85 mg/kg daily for 7 days eliminated clinical signs and infection in a closed breeding colonic of transgenic mice and after 14 days of treatment there were no detectable carriers when the colony was screened 4 weeks later (Matsumiya and Lavoie, 2003).…”

Prevalence and analysis of Pseudomonas aeruginosa in chinchillas

“…These results suggested that the treatment of the antibiotics was led to the reduced susceptibility of the isolate. In laboratory rodents, gentamicin and enrofloxacin have been generally used for the treatment of infectious diseases presumably caused by P. aeruginosa or another Gram-negative bacterium [10,25,26], while fluoroquinolones, carbapenems, and cephalosporins, such as ciprofloxacin, imipenem, and cefepime, may not be used for any bacterial infection in laboratory rodents in Japan and are strictly reserved for human use. In our study, no anamneses were found and none of antibiotics were administered in pet chinchillas.…”

Enrofloxacin—The Ruthless Killer of Eukaryotic Cells or the Last Hope in the Fight against Bacterial Infections?

“…For Salmonella strains, the MIC value varied between 0.06 and 0.25 µg/mL [ 60 , 61 ]. Significantly higher MIC values were obtained for Pseudomonas aeruginosa strains, ranging between 1 and 4 mg/mL [ 60 , 62 ].…”