Comparison of the protective effect of dipyridamole and acetylsalicylic acid on long-term histologic damage in a rat model of testicular ischemia-reperfusion injury

Karagüzel, Ersagun; Kutlu, Ömer; Yuluğ, Esin; Munğan, Sevdegül; Kazaz, İlke Onur; Tok, Doğan Sabri; Özgür, Güner Kemal

doi:10.1016/j.jpedsurg.2012.01.078

Cited by 38 publications

(25 citation statements)

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“…The accumulation of toxic oxide causes apoptosis in germ cells [14, 20]. Moreover, it has been revealed that apoptotic cells were observed in the spermatogonia and other spermatogenic cells in testicular I-R [21, 34]. We confirmed that apoptotic cells were specially observed in spermatogonia and spermatocytes.…”

Section: Discussionsupporting

confidence: 67%

Proteomic Analysis of Testicular Ischemia-Reperfusion Injury in Rats

Ouh

Seo

Shah

et al. 2014

The Journal of Veterinary Medical Science

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Testicular torsion is a urological emergency that leads to serious testicular damage and male infertility. We performed this study to identify specific proteins that are differentially expressed in response to testicular torsion and detorsion-induced ischemia-reperfusion (I-R) injury. Adult male rats were divided into two groups: a sham-operated group and a testicular I-R group. Testicular torsion was induced by rotating the left testis 720° in a clockwise direction for 1 hr, and then, detorsion was performed for 24 hr. After this testicular tissues were collected, protein analysis was performed using two-dimensional gel electrophoresis and Western blot analyses. Testicular I-R injury resulted in serious histopathologic damage to the germinal cells in the seminiferous tubules and increased the number of TUNEL-positive cells in testicular tissue. Specific protein spots with a greater than 2.5-fold change in intensity between the sham-operated and testicular I-R groups were identified by mass spectrometry. Among these proteins, levels of peroxiredoxin 6, thioredoxin, heterogeneous nuclear ribonucleoproteins, ubiquitin carboxyl terminal hydrolase isozyme L5 and zinc finger AN1-type domain 3 were decreased in the testicular I-R group compared to the sham-operated group. Moreover, Western blot analysis clearly showed the decrease of these proteins in the testicular I-R group. These proteins have spermatogenesis and anti-oxidative functions. These findings suggest that testicular I-R results in cell death due to altered expression of several proteins with spermatogenesis and anti-oxidation functions.

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Section: Discussionsupporting

confidence: 67%

Proteomic Analysis of Testicular Ischemia-Reperfusion Injury in Rats

Ouh

Seo

Shah

et al. 2014

The Journal of Veterinary Medical Science

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“…Apoptotic and normal cell numbers in 10 tubules in each slide were counted using Image-Pro Plus version 4.5 software (Media Cybernetics Inc, Silver Spring, MD, USA) at x 400 magnification. Apoptotic index was calculated as total TUNEL positive spermatogenetic cell number divided by total normal spermatogenetic cell number [35]. …”

Section: Methodsmentioning

confidence: 99%

p,p’-DDT induces testicular oxidative stress-induced apoptosis in adult rats

Marouani

Hallègue

Sakly

et al. 2017

Reprod Biol Endocrinol

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BackgroundThe 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (p,p’-DDT) is a known persistent organic pollutant and male reproductive toxicant. The present study is designed to test the hypothesis that oxidative stress mediates p,p’-DDT-induced apoptosis in testis.MethodsMale Wistar rats received an intraperitoneal (ip) injection of the pesticide at doses of 50 and 100mg/kg for 10 consecutive days. The oxidative stress was evaluated by biomarkers such lipid peroxidation (LPO) and metallothioneins (MTs) levels. Antioxidant enzymes activities was assessed by determination of superoxide dismutase (SOD), catalase (CAT) and hydrogen peroxide (H2O2) production. In addition, glutathione-dependent enzymes and reducing power in testis was evaluated by glutathione peroxidase (Gpx), glutathione reductase (GR), glutathione S-transferase (GST) activities and reduced and oxidized glutathione (GSH - GSSG) levels. Apoptosis was evaluated by DNA fragmentation detected by agarose gel electrophoresis. Germinal cells apoptosis and the apoptotic index was assessed through the TUNEL assay.ResultsAfter 10 days of treatment, an increase in LPO level and H2O2 production occurred, while MTs level, SOD and CAT activities were decreased. Also, the Gpx, GR, GST, and GSH activities were decreased, whereas GSSG activity was increased. Testicular tissues of treated rats showed pronounced degradation of the DNA into oligonucleotides as seen in the typical electrophoretic DNA ladder pattern. Intense apoptosis was observed in germinal cells of DDT-exposed rats. In addition, the apoptotic index was significantly increased in testis of DDT-treated rats.ConclusionsThese results clearly suggest that DDT sub-acute treatment causes oxidative stress in rat testis leading to apoptosis.

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“…Semen analyses as well as oxidative stress, histopathological and blood parameters evaluations after testicular torsion surgical treatments in experimental studies have shown marked abnormalities in both testes compared to normal standard values [3][4][5][6][7][8]. …”

Section: Short Communicationmentioning

confidence: 99%

“…Based on this concept, several pharmacological agents as well as surgical interventions have been examined to prevent or reverse ischemia-reperfusion induced fertility problems [5][6][7][8][9][10][11][12].…”

Section: Short Communicationmentioning

confidence: 99%

Testicular Torsion Treatment as a Male Reproductive Health Concern

Jalali¹,

Behfar²,

Nourian³

2016

J Trauma Treat

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Short CommunicationTesticular torsion is a true urological emergency and its annual incidence in men/boys younger than 25 years is 1 in 4000. This acute condition occurs most often (40% to 65%) in the neonatal period and around puberty [1].Testicular salvage rates following appropriate diagnostic and surgical managements range from 42% to 88% [2]. The main pathophysiological mechanisms underlying bilateral testicular damages following torsion-detorsion have been shown to be related to ischemia-reperfusion [3][4][5].Semen analyses as well as oxidative stress, histopathological and blood parameters evaluations after testicular torsion surgical treatments in experimental studies have shown marked abnormalities in both testes compared to normal standard values [3][4][5][6][7][8].

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Comparison of the protective effect of dipyridamole and acetylsalicylic acid on long-term histologic damage in a rat model of testicular ischemia-reperfusion injury

Cited by 38 publications

References 43 publications

Proteomic Analysis of Testicular Ischemia-Reperfusion Injury in Rats

Proteomic Analysis of Testicular Ischemia-Reperfusion Injury in Rats

p,p’-DDT induces testicular oxidative stress-induced apoptosis in adult rats

Testicular Torsion Treatment as a Male Reproductive Health Concern

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