Comparison of the response using ICR mice derived from three different sources to ethanol/hydrochloric acid-induced gastric injury

Abstract

Animal models for gastric ulcers produced by physical, pharmacological and surgical methods have been widely employed to evaluate therapeutic drugs and investigate the mechanism of action of this disease. ICR mice were selected to produce this model, even though several mice and rats have been widely used in studies of gastric ulcers. To compare the responses of ICR mice obtained from three different sources to gastric ulcer inducers, alterations in gastric injury, histopathological structure, and inflammation were measured in Korl:ICR (Korea NIFDS source), A:ICR (USA source) and B:ICR (Japan source) treated with three concentrations of ethanol (EtOH) (50, 70, and 90%) in 150 mM hydrochloric acid (HCl) solution. Firstly, the stomach lesion index gradually increased as the EtOH concentration increased in three ICR groups. Moreover, a significant increase in the level of mucosal injury, edema and the number of inflammatory cells was similarly detected in the EtOH/HCl treated group compared with the vehicle treated group in three ICR groups. Furthermore, the number of infiltrated mast cells and IL-1β expression were very similar in the ICR group derived from three different sources, although some differences in IL-1β expression were detected. Especially, the level of IL-1β mRNA in 50 and 90EtOH/HCl treated group was higher in Korl:ICR and A:ICR than B:ICR. Overall, the results of this study suggest that Korl:ICR, A:ICR and B:ICR derived from different sources have an overall similar response to gastric ulcer induced by EtOH/HCl administration, although there were some differences in the magnitude of their responses.

Comparative analysis of basal locomotor activity-related metabolic phenotypes between C57BL/6 mice and ICR mice substrains derived from three different sources

“…Among the rodent strains supplied by major providers, C57BL/6 and ICR mice are commonly used for research in aging, toxicology, pathology, and physiology [ 3 9 10 ]. Several studies indicated that RMR differs depending on the strain, gender, weight gain, growth rate, and other conditions derived from genetic and circumstantial variables [ 2 7 11 ].…”

“…Other studies approached the RMR-related analysis from the perspective of feeding, activity, and chemical substance administration, examining the effects of these variables within a strain as well as between strains [ 11 12 13 ]. Recently, studies on Korl mice (a domestic mouse strain) reported that they show similar responses to chemical substances [ 9 14 ]. However, no scientific studies have analyzed the metabolic phenotypes of mice substrains derived from three different origins, especially in C57BL/6Korl and Korl:ICR mice.…”

Comparison of commonly used ICR stocks and the characterization of Korl:ICR

“…The physiological and hematological data values had consistency in the study. Song et al (2016) suggested that three ICR groups, the Korl:ICR, A:ICR and B:ICR derived from different sources have an overall similar response of body weight, histopathological structure and inflammation to gastric ulcer inducers [25]. Alcohol induced ulcer model comparison assessment for reviewing the characteristics of the Korl:ICR were having similar result.…”

Comparison of therapeutic responses to an anticancer drug in three stocks of ICR mice derived from three different sources

“…In a genome-wide single nucleotide polymorphism (SNP) analysis, unique genetic variation was identified in Korl:ICR mice when compared with two ICR stocks derived from different sources [ 8 ]. However, Korl:ICR mice showed similar overall responses to gastric ulcers induced by EtOH/HCl administration compared to those in A:ICR and B:ICR mice stocks derived from different sources [ 9 ]. Also, similar responses to loperamide (Lop)-induced constipation in the transverse colon were detected in three ICR stocks (Korl:ICR, A:ICR, and B:ICR) derived from three difference sources [ 10 ].…”