Comparison of the sequential simplex method and linear solvent strength theory in HPLC gradient elution optimization of multicomponent flavonoid mixtures

Dondi, Francesco; Kahie, Y. D.; Lodi, G.; Reschiglian, Pierluigi; Pietrogrande, C.; Bighi, C.; Cartoni, G.P.

doi:10.1007/bf02311411

Cited by 20 publications

(2 citation statements)

References 12 publications

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“…Apigenin does not appear to accumulate in chamomile flowers in the free form (Schreiber et al, 1990), and there is evidence to suggest that the aglycone is actually a product of post-harvest degradation (Maier et al, 1991). Besides apigenin and its derivatives, chamomile flowers also contain luteolin, quercetin, patuletin (and their glycosides), herniarin and umbelliferone (Redaelli et al, 1981a) as well as several phenylpropanoid derivatives (Dondi et al, 1987;Mulinacci et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Validation of a capillary electrophoresis method for the quantitative determination of free and total apigenin in extracts of Chamomilla recutita

Fonseca

Tavares

2004

Phytochemical Analysis

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A capillary electrophoretic method for the quantification of free and total apigenin in methanolic, ethanolic and glycolic extracts of Chamomilla recutita L. Rauschert (Asteraceae) is described. The method was validated for measurement of apigenin in the range 5.00-300 microg/mL (r2 = 0.993) and showed coefficients of intra-day (replicability) and inter-day (repeatability) variability of better than 2%. The limits of detection and quantification were 3.80 and 11.5 microg/mL, respectively, and the average recovery was 102.0 +/- 0.8% at three concentration levels of apigenin. Free and total apigenin contents in the extracts were, respectively, determined as 106 and 903 microg/g (methanolic extract), 77 and 817 microg/g (ethanolic extract) and 11.0 and 247 microg/g (glycolic extract).

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Section: Introductionmentioning

confidence: 99%

Validation of a capillary electrophoresis method for the quantitative determination of free and total apigenin in extracts of Chamomilla recutita

Fonseca

Tavares

2004

Phytochemical Analysis

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“…Nine compounds were confirmed via a comparison with the standards (37, 42–44, 57, 59, 63, 65, and 66) and the others were tentatively identified via examination of the MS spectra. Some flavonoids specific for chamomile, such as 6-methoxyapigenin or hispidulin, 6-methoxyluteolin or nepetin [ 22 ], 6-hydroxyquercetin or quercetagetin [ 23 ], and 6-methoxyquercetin or patuletin [ 24 ], were also found in this study. Compound 50 was marked as an apigenin derivative, because its exact structure could not be suggested.…”

Section: Resultsmentioning

confidence: 56%

New Biological and Chemical Insights into Optimization of Chamomile Extracts by Using Artificial Neural Network (ANN) Model

Cvetanović

Radosavljević

Zengin

et al. 2023

Plants

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Chamomile is one of the most consumed medicinal plants worldwide. Various chamomile preparations are widely used in various branches of both traditional and modern pharmacy. However, in order to obtain an extract with a high content of the desired components, it is necessary to optimize key extraction parameters. In the present study, optimization of process parameters was performed using the artificial neural networks (ANN) model using a solid-to-solvent ratio, microwave power and time as inputs, while the outputs were the yield of the total phenolic compounds (TPC). Optimized extraction conditions were as follows: a solid-to-solvent ratio of 1:80, microwave power of 400 W, extraction time of 30 min. ANN predicted the content of the total phenolic compounds, which was later experimentally confirmed. The extract obtained under optimal conditions was characterized by rich composition and high biological activity. Additionally, chamomile extract showed promising properties as growth media for probiotics. The study could make a valuable scientific contribution to the application of modern statistical designs and modelling to improve extraction techniques.

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Retention behaviour of selected flavonoid compounds in NP-HPLC

et al. 1989

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Comparison of the sequential simplex method and linear solvent strength theory in HPLC gradient elution optimization of multicomponent flavonoid mixtures

Cited by 20 publications

References 12 publications

Validation of a capillary electrophoresis method for the quantitative determination of free and total apigenin in extracts of Chamomilla recutita

Validation of a capillary electrophoresis method for the quantitative determination of free and total apigenin in extracts of Chamomilla recutita

New Biological and Chemical Insights into Optimization of Chamomile Extracts by Using Artificial Neural Network (ANN) Model

Retention behaviour of selected flavonoid compounds in NP-HPLC

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