Comparison of the Volatile Composition of Stachys pubescence Oils 
 Obtained by Hydro Distillation and Steam Distillation

Biglar, Mahmoud; Ardekani, Mohammad Reza Shams; Khanavi, Mahnaz; Shafiee, Abbas; Rustaiyan, Abdolhossein; Salimpour, Fahimeh; Farjadmand, Fatemeh

doi:10.3923/pjbs.2014.942.946

Cited by 5 publications

(2 citation statements)

References 22 publications

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“…This genus is represented by 34 species in Iran of which13 are endemic( 5 6 ). The presence of flavonoids, phenylethanoid glycosides, diterpenes, saponins, terpenoids, and steroids have been reported in Stachys species( 7 8 ).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic effect of methanolic extract, alkaloid and terpenoid fractions of Stachys pilifera against HT-29 cell line

et al. 2018

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Stachys pilifera (S. pilifera) Benth (Lamiaceae) is used in traditional medicine to treat a variety of diseases. Despite some reports on the antitumor effects of some species of this genus, anticancer activity of S. pilifera has not been yet reported. Here, we examined the cytotoxic effect and cell death mechanisms of methanolic extract of S. pilifera and its alkaloid and terpenoid fractions on the HT-29 colorectal cell line. HT-29 cells were cultivated and then incubated in the methanolic extract of S. pilifera and its fractions at various concentrations for 24 h. Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Morphology of cells was evaluated by contrast microscopy. Furthermore, effects of the tested extract and fractions were tested on some regulators of cell death and proliferation such as caspase-8, caspase-9, nuclear factor-κB (NF-κB), and nitric oxide (NO). Cisplatin was used as positive control. The estimated IC50 values of the methanolic extract, alkaloid and terpenoid fractions, and cisplatin against HT29 cell after 24 h were determined to be 612, 48.12, 46.44, and 4.02 μg/mL, respectively. Morphological changes such as plasma membrane blebbing, cell size reduction, and apoptotic bodies were observed in cells faced with the extract and fractions. S. pilifera extract and its fractions induced apoptosis through inhibition of NF-κB, NO, and activation of caspase-8 and caspase-9. Data showed considerable cytotoxic and antiproliferative effects of S. plifera on colorectal cell line through induction of apoptosis. These findings provide a basis for the therapeutic potential of S. pilfera in the treatment of colon cancer.

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Section: Introductionmentioning

confidence: 99%

Cytotoxic effect of methanolic extract, alkaloid and terpenoid fractions of Stachys pilifera against HT-29 cell line

et al. 2018

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“… 2006 ; Biglar et al. 2014 ). Several biological studies have shown consideration anti-inflammatory, antioxidant, antibacterial and anti-hepatitis effects of this genus (Maleki et al.…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective effect of Stachys pilifera ethanol extract in carbon tetrachloride-induce hepatotoxicity in rats

Kokhdan

Ahmadi

Sadeghi

et al. 2017

Pharmaceutical Biology

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Context:Stachys pilifera Benth (Lamiaceae) has long been used to treat infectious diseases, respiratory and rheumatoid disorders in Iranian folk medicine. Antitumor and antioxidant activity of the plant have been reported.Objective: The study was designed to assess the hepatoprotective activity of ethanol extract of Stachys pilifera in carbon tetrachloride (CCl4)-induced hepatotoxicity in rats.Materials and methods: The rats were randomly divided into six equal groups (n = 7). Group I was treated with normal saline; Group II received CCl4 (1 mL/kg. i.p., twice a week) for 60 consecutive days; Groups III, IV and V were given CCl4 plus Stachys pilifera (100, 200 and 400 mg/kg/d,p.o.); Group VI received the extract (400 mg/kg/d, p.o.). Histopathological analysis and measurement of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), malondialdehyde (MDA), total protein (TP) and albumin (ALB) were performed.Results: CCl4 caused a significant increase in the serum levels of AST, ALT, ALP and MDA as well as decreased ALB, and TP serum levels (p < 0.001). The extract (200 and 400 mg/kg/d) significantly normalized the CCl4-elevated levels of ALT, AST, ALP and MDA (p < 0.001). The extract (200 and 400 mg/kg/d) also increased the serum levels of TP compared to CCl4 group (p< 0.01). The extract (200 and 400 mg/kg/d) also decreased the histological injuries (inflammation and fatty degeneration) by CCl4.Discussion: The results revealed that the Stachys pilifera extract could provide considerable protection against CCl4 hepatotoxicity in rats that may be related to its antioxidant properties.

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Chemical composition and citral content of essential oil of lemongrass (Cymbopogon citratus (DC.) Stapf) leaf waste prepared with various production methods

Khasanah,

Ariviani,

Purwanto

et al. 2025

Journal of Agriculture and Food Research

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Comparison of the Volatile Composition of Stachys pubescence Oils Obtained by Hydro Distillation and Steam Distillation

Cited by 5 publications

References 22 publications

Cytotoxic effect of methanolic extract, alkaloid and terpenoid fractions of Stachys pilifera against HT-29 cell line

Cytotoxic effect of methanolic extract, alkaloid and terpenoid fractions of Stachys pilifera against HT-29 cell line

Hepatoprotective effect of Stachys pilifera ethanol extract in carbon tetrachloride-induce hepatotoxicity in rats

Chemical composition and citral content of essential oil of lemongrass (Cymbopogon citratus (DC.) Stapf) leaf waste prepared with various production methods

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