2016
DOI: 10.1186/s12917-016-0828-z
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Comparison of three different methods for the quantification of equine insulin

Abstract: BackgroundExact analysis of equine insulin in blood samples is the key element for assessing insulin resistance or insulin dysregulation in horses. However, previous studies indicated marked differences in insulin concentrations obtained from sample analyses with different immunoassays. Most assays used in veterinary medicine are originally designed for use in human diagnostics and are based on antibodies directed against human insulin, although amino acid sequences between equine and human insulin differ. Spe… Show more

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Cited by 61 publications
(56 citation statements)
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“…It has been well described that, as most insulin assays are immunoassays, the amount of immunoreactive insulin detected by each assay can vary and that a simple correction factor cannot be used to easily compare concentrations obtained with 1 assay with concentrations obtained with another assay. 17,24 Therefore, this limitation was addressed by using the percent change in insulin concentration rather than the actual measured insulin concentration. An additional limitation was the use of a 24-hour washout period between alpha-2-agonist testing periods.…”
Section: Discussionmentioning
confidence: 99%
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“…It has been well described that, as most insulin assays are immunoassays, the amount of immunoreactive insulin detected by each assay can vary and that a simple correction factor cannot be used to easily compare concentrations obtained with 1 assay with concentrations obtained with another assay. 17,24 Therefore, this limitation was addressed by using the percent change in insulin concentration rather than the actual measured insulin concentration. An additional limitation was the use of a 24-hour washout period between alpha-2-agonist testing periods.…”
Section: Discussionmentioning
confidence: 99%
“…Plasma was collected immediately, whereas blood was allowed to clot for 45 minutes at room temperature, centrifuged, and serum was extracted. Depending on the institution, insulin was measured using a radioimmunoassay (Institution 1) or a chemiluminescent assay (Institution 2) previously validated in horses 16,17 . Blood glucose was measured using a glucohexokinase colorimetric assay as previously described 14 .…”
Section: Methodsmentioning
confidence: 99%
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“…Farm animal and pet welfare is the main goal of veterinary medicine for both economic and affection reasons. Nowadays, the performance of animal diagnostic serology is often suboptimal, mostly due to the low availability of commercial species‐specific immunoassays . Even though clinical care may capitalize from what has already been discovered by human system biology, dedicated biomarker discovery and validation efforts would be needed for improving veterinary diagnostics .…”
Section: Numbers Of Identified Proteins Peptides Psms and Search Imentioning
confidence: 99%
“…species-specific immunoassays. [1] Even though clinical care may capitalize from what has already been discovered by human system biology, dedicated biomarker discovery and validation efforts would be needed for improving veterinary diagnostics. [2] Nevertheless, compared to humans, biomarker discovery studies directly performed on animal specimens lag considerably behind.…”
Section: Doi: 101002/pmic201800191mentioning
confidence: 99%