Comparison of three different methods for measurement of tissue platinum level

Abstract

We attempted to make a comparison of three methods for tissue platinum; atomic absorption spectrometry (AAS), inductively coupled plasma atomic emission spectrometry (ICP-AES), and inductively coupled plasma mass spectrometry (ICP-MS). The determination limits were 0.05 ng/mL on ICP-MS, 50 ng/mL on ICP-AES, and 200 ng/mL on AAS, and the recovery rates were 97.7 +/- 6.9% on ICP-MS, 69.0 +/- 3.0% on ICP-AES, and 102.4 +/- 4.0% on AAS, respectively. Platinum was detected by ICP-AES and ICP-MS in human vertebrae, but the level was higher by ICP-AES than by ICP-MS. In the mouse kidney treated with cisplatin, platinum was detected by ICP-MS, but not by ICP-AES. As cadmium gives the absorption peak close to platinum, cadmium was measured together with platinum by ICP-AES in the vertebrae. From these, ICP-MS is the most sensitive for measurement at tissue platinum. The sensitivity of ICP-AES looks worse for measuring the tissue platinum, and it is necessary to take care of the contaminant of metals, especially cadmium. AAS is not suitable for measurement of tissue platinum as in the vertebrae and kidneys, because platinum was not detectable by AAS.

Salicylate reduces cisplatin nephrotoxicity by inhibition of tumor necrosis factor-α

“…Baker, Phillipsburg, NJ, USA) for one hour at 100 • C. The digested samples were diluted with high-purity water and subjected to inductively coupled plasma/mass spectroscopy (ICP/MS) (Elan 6000; Perkin-Elmer, Boston, MA, USA) to determine the platinum content. ICP/MS has been shown to be superior to either atomic absorption spectrometry or inductively coupled plasma atomic emission spectrometry for the measurement of tissue platinum [16].…”

The application of inductively coupled plasma mass spectrometry in pharmaceutical and biomedical analysis

“…The detection limit for cisplatin was found to be 0.1 ng. Three methods, AAS, ICP-AES and ICP-MS, for tissue platinum measurement were attempted by Minami et al [33]. At last they had a conclusion that ICP-MS is the most suitable and sensitive method because of its low detection limit for determination of tissue platinum.…”

The application of inductively coupled plasma mass spectrometry in clinical pharmacological oncology research

“…The digestion procedure can be performed in several ways, varying from heating at high temperatures in concentrated HNO 3 (Tothill, Matheson, & Smyth, 1990;Tothill et al, 1992a,b;Minami et al, 1994;Minami, Ichii, & Okazaki, 1995;Screnci et al, 2000;Liang & Huang, 2002;Siim et al, 2003;Ghezzi et al, 2004;McDonald et al, 2005;Centerwall et al, 2006;Rice et al, 2006;Samimi et al, 2007) to more complex procedures such as digestion with concentrated HNO 3 and 30% H 2 O 2 (Ding et al, 1999;Bible et al, 2000) at elevated temperatures or microwave digestion with HNO 3 , H 2 O 2 , and HCl (Hann et al, 2003a). Perry and Balazs (1994) compared the digestion of cells with sodium hydroxide with the sonication of cells followed by digestion with concentrated HNO 3 .…”