Comparison of three stool antigen tests for Helicobacter pylori detection

Andrews, J A; Marsden, Brian D.; Brown, Darron R.; Wong, Voi Shim; Wood, Ellena; Kelsey, Moira

doi:10.1136/jcp.56.10.769

Cited by 36 publications

(22 citation statements)

References 6 publications

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“…This is in accordance with previous studies on this test, with reports of sensitivity and specificity in the range of 88.5%-98% and 93.8%-99%, respectively (23,27,28). These studies have, however, not addressed the effect of PPIs on test performance.…”

Section: Discussionsupporting

confidence: 92%

“…In general, PPI therapy is known to interfere with H. pylori diagnosis; it diminishes H. pylori load and enzyme activity, leading to lower sensitivity of both invasive tests and urea breath tests (20). The use of PPIs has been shown to affect the outcome of the polyclonal antibody-based H. pylori stool antigen test (21)(22)(23). However, there are no published data on the effect of PPI treatment on test results of monoclonal antibody-based tests.…”

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confidence: 99%

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Accuracy of a monoclonal antibody-based stool antigen test in the diagnosis of Helicobacter pylori infection

Asfeldt¹,

Løchen²,

Straume³

et al. 2004

Scandinavian Journal of Gastroenterology

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Section: Discussionsupporting

confidence: 92%

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confidence: 99%

Accuracy of a monoclonal antibody-based stool antigen test in the diagnosis of Helicobacter pylori infection

Asfeldt¹,

Løchen²,

Straume³

et al. 2004

Scandinavian Journal of Gastroenterology

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“…Although this permits us to think that this large difference is related to antigenic diversity between the two strains, it would be a premature conclusion, since the methods of evaluation were different; furthermore, more analysis with larger numbers of H. pylori strains is required to determine the analytical sensitivity for an HpSA test. A variety of experiments have been performed with HpSA tests, including Premier Platinum HpSA, Immuno Card STAT HpSA, and FemtoLab H. pylori CnX (1,2,12,15,16,18,20,(39)(40)(41). However, no uniformity was observed in different populations in regard to the cutoff level required to consider the test positive or negative.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, a low-cost, rapid diagnostic technique may be useful for the management of H. pylori infection in children and adolescents from developing regions. The H. pylori stool antigen test has been introduced as a noninvasive, simple, relatively inexpensive, and reliable assay in the diagnosis of H. pylori infection of gastritis or peptic ulcer in adults and children (1,2,15,17). Although it is not yet at the level to replace histology as a gold standard, it may be a promising tool in detection of H. pylori infection and could be used in the follow-up of adults and children undergoing antibiotic therapy (5,8,12,18,20,23,24,29,32,33,38,39,41).…”

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confidence: 99%

Application of a Stool Antigen Test To Evaluate the Incidence ofHelicobacter pyloriInfection in Children and Adolescents from Tehran, Iran

Falsafi

Valizadeh

Sepehr

et al. 2005

Clin Vaccine Immunol

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Helicobacter pylori infection is acquired mainly in childhood, especially in developing countries, where a low-cost, rapid diagnostic technique which is reliable for all age groups may be useful for the management of H. pylori infection. For this purpose, we used an HpSA test (Equipar) to detect H. pylori infection in children and adolescents from Tehran, Iran. Thirty-five children who were positive or negative for H. pylori infection by endoscopy-based tests were used as positive and negative controls for the HpSA test. Stools were collected from 430 randomly selected children and adolescents (4 to 18 years old) from southwest, near the center, and northwest of Tehran. A questionnaire that included presence of recurrent abdominal pain (RAP), family history of infection and/or peptic ulcer disease (PUD), and income of parents was completed. A good agreement was found between the results of endoscopy-based tests and those of the HpSA test; the sensitivity and specificity of the Equipar-HpSA test were 100% and 83.4%, respectively. Among 430 children and adolescents, 47% were positive by the HpSA test, of whom 82% had RAP. No difference in incidence was observed between the two sexes; the various categories of age showed an increasing incidence, ranging from 24% (ages 4 to 6) to 58% (ages 16 to 18). The rate of infection in children and adolescents from the southwest was significantly higher (70%) than the rate in those from the northwest (32%), and a family history of H. pylori infection or PUD was observed in 59% of the HpSA positive subjects. The HpSA test is a useful test to detect H. pylori infection in children and adolescents from developing countries.Helicobacter pylori infection is acquired mainly in childhood, especially in developing countries (40), where the influence of socioeconomic factors on the prevalence of H. pylori infection has been shown (11,30,37). Many investigators have studied the criteria for diagnosis and treatment of children infected by H. pylori, but association of symptoms with H. pylori infection in children presenting with nonulcer dyspepsia is controversial (3,14,22). One important controversy relates to the presence of recurrent abdominal pain (RAP) in children, where an important association was observed between RAP and H. pylori infection in some populations (4,10,19,27,28,31,34). Although endoscopy-based tests are the best methods to diagnose active H. pylori infection, their application in children is more difficult and unpleasant than in adults. Moreover, in developing regions, for socioeconomic reasons, most infected children are not diagnosed and/or treated for H. pylori infection. To circumvent these difficulties, a noninvasive test with reliability for all age groups of children and adolescents is required. Among the noninvasive methods, serological tests cannot be applied to young children because of low sensitivity. In addition, the 13 C urea breath test is cumbersome, expensive, and consequently unavailable in certain countries; furthermore, it is not reliable in very...

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“…The fecal antigen test is a noninvasive method of diagnosis of H. pylori antigens in the feces of patients suspected of having an active H. pylori infection (Andrews et al 2003). Studies have proved the accuracy of the fecal antigen test named the H. pylori stool antigen (HpSA) test for detection of H. pylori infection (Iranikhah et al 2013).…”

Section: Noninvasive Testsmentioning

confidence: 99%

Biomarkers and diagnostic tools for detection of Helicobacter pylori

Khalilpour

Kazemzadeh-Narbat

Tamayol

et al. 2016

Appl Microbiol Biotechnol

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Helicobacter pylori is responsible for worldwide chronic bacterial infection in humans affecting approximately half of the world's population. H. pylori is associated with significant morbidity and mortality including gastric cancer. The infection has both direct and indirect impacts on economic and overall well-being of patients; hence, there is a great need for diagnostic markers that could be used in the development of diagnostic kits. Here, we briefly review general aspects of H. pylori infection and the diagnostic biomarkers used in laboratory tests today with a focus on the potential role of microfluidic systems in future immunodiagnosis platforms.

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Comparison of three stool antigen tests for Helicobacter pylori detection

Cited by 36 publications

References 6 publications

Accuracy of a monoclonal antibody-based stool antigen test in the diagnosis of Helicobacter pylori infection

Accuracy of a monoclonal antibody-based stool antigen test in the diagnosis of Helicobacter pylori infection

Application of a Stool Antigen Test To Evaluate the Incidence ofHelicobacter pyloriInfection in Children and Adolescents from Tehran, Iran

Biomarkers and diagnostic tools for detection of Helicobacter pylori

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