Comparison of two clean-up principles for determination of trichothecenes in grain extract

Radová, Zuzana; Holadová, K.; Hajšlová, Jana

doi:10.1016/s0021-9673(98)00868-1

Cited by 49 publications

(32 citation statements)

References 21 publications

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“…C 8 , C 18 ), strong cation or anion exchangers (SCX, SAX) or polymeric materials with combined properties. Modern clean-up procedures employ multifunctional MycoSep Õ (Krska 1998;Radová et al 1998;Biselli and Hummert 2005;Ren et al 2007) or immunoaffinity columns (IAC) (Krska 1998), although these methods are more expensive than conventional clean-up methodologies. MycoSep Õ columns contain a mixture of charcoal, ion-exchange resins and other materials and are suitable for aflatoxins, trichothecenes, ochratoxins, zearalenone, moniliformin and patulin (Romerlabs 2007).…”

Section: Sample Preparation and Clean-upmentioning

confidence: 99%

Mycotoxin analysis: An update

Krska¹,

Schubert-Ullrich²,

Molinelli³

et al. 2008

Food Additives & Contaminants: Part A

312

215

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Mycotoxin contamination of cereals and related products used for feed can cause intoxication, especially in farm animals. Therefore, efficient analytical tools for the qualitative and quantitative analysis of toxic fungal metabolites in feed are required. Current methods usually include an extraction step, a clean-up step to reduce or eliminate unwanted co-extracted matrix components and a separation step with suitably specific detection ability. Quantitative methods of analysis for most mycotoxins use immunoaffinity clean-up with high-performance liquid chromatography (HPLC) separation in combination with UV and/or fluorescence detection. Screening of samples contaminated with mycotoxins is frequently performed by thin layer chromatography (TLC), which yields qualitative or semi-quantitative results. Nowadays, enzyme-linked immunosorbent assays (ELISA) are often used for rapid screening. A number of promising methods, such as fluorescence polarization immunoassays, dipsticks, and even newer methods such as biosensors and non-invasive techniques based on infrared spectroscopy, have shown great potential for mycotoxin analysis. Currently, there is a strong trend towards the use of multi-mycotoxin methods for the simultaneous analysis of several of the important Fusarium mycotoxins, which is best achieved by LC-MS/MS (liquid chromatography with tandem mass spectrometry). This review focuses on recent developments in the determination of mycotoxins with a special emphasis on LC-MS/MS and emerging rapid methods.

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Section: Sample Preparation and Clean-upmentioning

confidence: 99%

Mycotoxin analysis: An update

Krska¹,

Schubert-Ullrich²,

Molinelli³

et al. 2008

Food Additives & Contaminants: Part A

312

215

View full text Add to dashboard Cite

show abstract

“…All the grain samples collected during the period of 1999-2001 were analysed for DON levels using gas chromatography coupled with electron capture detector (GC/ECD) for quantification of the target analyte (Radová et al 1998). Briefly, the homogenised ground cereal sample (10 g) was extracted with 100 ml acetonitrile:water mixture (84:16, v/v) by shaking it for one hour, MycoSep TM 225 SPE cartridge was used for the purification of the crude extract, trifluoroacetanhydride (TFAA) was used for derivatisation of DON prior to the determination.…”

Section: Determination Of Donmentioning

confidence: 99%

Occurrence of trichothecene mycotoxins in cereals harvested in the Czech Republic

Hajšlová¹,

Lancová²,

Sehnalová³

et al. 2007

Czech J. Food Sci.

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A large-scale survey of the natural occurrence of trichothecene mycotoxins in major cereals harvested in the Czech Republic was conducted during the years 1999–2001. In total, 198 cereal samples representing various wheat, barley, and rye cultivars were examined for deoxynivalenol (DON) using gas chromatography with electron capture detector (GC-ECD). Four years later, in 2005, the list of target analytes was fairly extended, 65 wheat and barley samples were analysed for seven trichothecene mycotoxins – deoxynivalenol (DON), nivalenol (NIV), fusarenon-X (Fus-X), 15-acetyldeoxynivalenol and 3-acetyldeoxynivalenol (ADONs), HT-2 toxin (HT-2). and T-2 toxin (T-2) by high performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Trichothecenes represented mainly by DON were detected in almost all grain samples, its mean levels were the highest in the year 1999 which was characterised by very humid conditions during the growing season. The maximum concentration set in Commission Regulation EC (No. 856/2005) for DON (1250 µg/kg) in unprocessed cereals was exceeded only in two of all the samples analysed.

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“…For DON, nivalenol and T2-toxin, two main types of method are reported; both advise gas chromatography using either and electron capture detector (ECD) or a MS detector [54][55][56][57] . A clean up (Romer Myco-sep) is mentioned by two labs.…”

Section: How To Analyse Them?mentioning

confidence: 99%

Survey on the Analysis of Mycotoxins

2004

Journal of the Institute of Brewing

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Comparison of two clean-up principles for determination of trichothecenes in grain extract

Cited by 49 publications

References 21 publications

Mycotoxin analysis: An update

Mycotoxin analysis: An update

Occurrence of trichothecene mycotoxins in cereals harvested in the Czech Republic

Survey on the Analysis of Mycotoxins

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