Comparison of two freezing methods for human immature oocytes with cumulus or cumulus free: Slow freezing versus vitrification (including the effect of Taxol)

Kyono, Koichi; Haigo, Kosuke; Fukunaga, Noritaka; Nakajo, Yukiko; Chiba, Setsuyo; Fuchinoue, Kohei

doi:10.1016/s0015-0282(02)03415-5

Cited by 3 publications

(1 citation statement)

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“…However, there have been different conclusions about whether cumulus cells can improve the survival rate and developmental competence of frozen-thawed oocytes. [5][6][7] It has been found that the survival rate is higher in vitrified-thawed human germinal vesicle-denuded oocyte (GV-DO) than germinal vesicle-cumulus oocyte complex (GV-COC), 7 suggesting that cumulus cells may limit the degree and speed of dehydration. However, Imoedehme and Sigue 8 have reported that the survival rate is significantly higher in frozen-thawed oocytes with cumulus than in frozen-thawed cumulus cell-free oocytes.…”

Section: Introductionmentioning

confidence: 99%

Effects of Cumulus Cells on Vitreous Cryopreservation of Human Mature Oocytes and Clinical Pregnancy Outcomes

et al. 2012

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The purpose of this study was to explore the effects of cumulus cells on vitreous cryopreservation of human mature oocytes and clinical pregnancy outcomes. The study was divided into group A (cumulus cells were removed from the oocytes before freezing) containing 24 participants and 193 oocytes and group B (cumulus cells were retained with the oocytes before freezing) containing 26 participants and 240 oocytes. Based on no significant differences in age, duration of infertility, infertile causes, and number of retrieved oocytes between both groups when oocytes were retrieved from infertile women, we found that the survival rate of post thaw oocytes (88% vs 58%), cleavage rate (80% vs 56%), and high-quality embryo rate (75% vs 59%) were significantly higher in group B than in group A. Under the conditions that there were no significant differences between the 2 groups in the general status of the participants undergoing embryo transfer, the embryo implantation rate (37% vs 15%) and the clinical pregnancy rate (50% vs 17%) were significantly higher in group B than in group A, all with Ps < .05. We conclude that the retention of cumulus cells can improve the developmental competence of vitrified-thawed human mature oocytes and clinical pregnancy outcomes.

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Section: Introductionmentioning

confidence: 99%

Effects of Cumulus Cells on Vitreous Cryopreservation of Human Mature Oocytes and Clinical Pregnancy Outcomes

et al. 2012

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Effects of pre‐treating in vitro‐matured bovine oocytes with the cytoskeleton stabilizing agent taxol prior to vitrification

Morató

Izquierdo

Albarracı́n

et al. 2007

Molecular Reproduction Devel

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The purpose of this study was to determine the efficacy of pre-treating mature bovine oocytes with Taxol before vitrification by the open pulled Straw method (OPS). We evaluated the effects of pre-treating the oocytes with 1 microM Taxol on chromosome organization, spindle morphology, cortical granule distribution and the ability of fertilized oocytes to develop to the blastocyst stage. After calf or cow oocyte vitrification without Taxol, significantly higher proportions of spindle abnormalities in the form of abnormal spindle structures or dispersed or decondensed chromosomes were observed compared to fresh control oocytes. In contrast, when we compared calf oocytes pre-treated with Taxol before vitrification with control calf oocytes, similar percentages of oocytes showing a normal spindle morphology were observed. The percentages of oocytes with a peripheral cortical granule (CG) distribution increased when the oocytes were pretreated with Taxol and vitrified, while oocytes vitrified without Taxol pre-treatment gave rise to higher cortical distribution percentages. Cleavage and blastocyst rates were significantly lower for vitrified versus untreated oocytes, both in cow and calf oocytes. Significantly higher cleavage rates were obtained when calf and cow oocytes were vitrified with Taxol. Pre-treatment with Taxol before cow oocyte vitrification yielded significantly higher blastocyst rates. Calf oocytes, however, were unable to develop to the blastocyst stage, irrespective of previous Taxol treatment. These results indicate that the pre-treatment of oocytes with Taxol before vitrification helps to reduce the damage induced by the cryopreservation process, and potentially improves the subsequent development of vitrified bovine oocytes. Summary sentence: Pre-treatment of oocytes with Taxol before vitrification helps to reduce the damage induced by vitrification and potentially improves the development of vitrified bovine oocytes.

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Vitrifying and warming of oocytes using Cryotop

Kyono¹,

Nakajo²,

Kumagai³

et al. 2007

Reproductive Medicine and Assisted Reproductive Techniques

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Comparison of two freezing methods for human immature oocytes with cumulus or cumulus free: Slow freezing versus vitrification (including the effect of Taxol)

Cited by 3 publications

References 0 publications

Effects of Cumulus Cells on Vitreous Cryopreservation of Human Mature Oocytes and Clinical Pregnancy Outcomes

Effects of Cumulus Cells on Vitreous Cryopreservation of Human Mature Oocytes and Clinical Pregnancy Outcomes

Effects of pre‐treating in vitro‐matured bovine oocytes with the cytoskeleton stabilizing agent taxol prior to vitrification

Vitrifying and warming of oocytes using Cryotop

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