Comparison of Two Methods for Assaying Reducing Sugars in the Determination of Carbohydrase Activities

Gusakov, Alexander V.; Kondratyeva, E. G.; Sinitsyn, A. P.

doi:10.1155/2011/283658

Cited by 208 publications

(115 citation statements)

References 23 publications

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“…The total phenolic compound and reducing sugar contents were analyzed according to previous reports. 17,18 In vitro antioxidant activity, including DPPH, ABTS, nitric oxide, and superoxide anion radical scavenging activities, was also investigated according to previous reports. [19][20][21][22] assessment of reaction yield by using IcP-Oes…”

Section: Green Synthesis Of Gm-aunps and Gm-agnpsmentioning

confidence: 99%

Asymmetric dumbbell-shaped silver nanoparticles and spherical gold nanoparticles green-synthesized by mangosteen (Garcinia mangostana) pericarp waste extracts

Park¹,

Ahn²,

Park³

2017

IJN

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Mangosteen ( Garcinia mangostana ) pericarp waste extract was used to synthesize gold and silver nanoparticles by a green strategy. The extract was both a reducing and stabilizing agent during synthesis. Phytochemical screening of the extract was conducted to obtain information regarding the presence/absence of primary and secondary metabolites in the extract. The in vitro antioxidant activity results demonstrated that the extract had excellent antioxidant activity, which was comparable to a standard (butylated hydroxy toluene). Spherical gold nanoparticles (gold nanoparticles green synthesized by mangosteen pericarp extract [GM-AuNPs]) with an average size of 15.37±3.99 to 44.20±16.99 nm were observed in high-resolution transmission electron microscopy (HR-TEM) images. Most interestingly, the silver nanoparticles (silver nanoparticles green synthesized by mangosteen pericarp extract [GM-AgNPs]) had asymmetric nanodumbbell shapes where one tail grew from a spherical head. The average head size was measured to be 13.65±5.07 to 31.08±3.99 nm from HR-TEM images. The hydrodynamic size of both nanoparticles tended to increase with increasing extract concentration. Large negative zeta potentials (−18.92 to −34.77 mV) suggested that each nanoparticle solution possessed excellent colloidal stability. The reaction yields were 99.7% for GM-AuNPs and 82.8% for GM-AgNPs, which were assessed by inductively coupled plasma optical emission spectroscopy. A high-resolution X-ray diffraction pattern confirmed the face-centered cubic structure of both nanoparticles. Based on phytochemical screening and Fourier transform infrared spectra, the hydroxyl functional groups of carbohydrates, flavonoids, glycosides, and phenolic compounds were most likely involved in a reduction reaction of gold or silver salts to their corresponding nanoparticles. The in vitro cytotoxicity (based on a water-soluble tetrazolium assay) demonstrated that GM-AgNPs were toxic to both A549 (a human lung cancer cell) and NIH3T3 (a mouse fibroblast cell). The cytotoxicity of GM-AgNPs on A549 cells was related to apoptotic cell death. However, GM-AuNPs did not show any significant cytotoxicity to either cell. These results suggest that GM-AuNPs have the potential to be drug delivery vehicles or carriers for pharmaceutical and biomedical applications.

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Section: Green Synthesis Of Gm-aunps and Gm-agnpsmentioning

confidence: 99%

Asymmetric dumbbell-shaped silver nanoparticles and spherical gold nanoparticles green-synthesized by mangosteen (Garcinia mangostana) pericarp waste extracts

Park¹,

Ahn²,

Park³

2017

IJN

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“…Carbohydrate levels were measured using the 3,5-dinitrosalicylic acid (DNS) method (14). Protein was measured via Lowry's method (15).…”

Section: Methodsmentioning

confidence: 99%

Hypolipidemic Effect of the Autoclaved Extract Prepared from Pea (Pisum sativum L.) Pods In Vivo and In Vitro

Inagaki

Nishimura

Iwata

et al. 2016

J Nutr Sci Vitaminol

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Summary By autoclaving, we obtained a polyphenol and dietary fiber from pea (Pisum sativum L.) pods in parallel without acid or alkali treatment or organic solvent extraction. Rats fed a high-sucrose (HS) diet containing 3% autoclaved extract (AE) for 4 wk exhibited significantly lower serum triglyceride and total cholesterol levels than rats fed a HS diet. AE and soluble dietary fiber (SDF) from AE exhibited pancreatic lipase inhibitory activity at 13.3 mg/mL in vitro. AE and insoluble dietary fiber (IDF) from AE adsorbed cholesterol. In total, 30% and 10% of a cholesterol micelle were significantly adsorbed by 2,000 mg of AE and 100 mg of IDF from AE in 7 mL, respectively. The amount of bifidobacteria in the cecum of the AE group was significantly increased compared with that in the HS group. These results suggest that AE has hypolipidemic, bifidogenic potential.

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“…Reducing sugar concentration was determined by 3, 5-dinitrosalicylic acid (DNS) assay (Gusakov et al, 2011).…”

Section: Methodsmentioning

confidence: 99%

Production of glucose oxidase and catalase by Aspergillus niger free and immobilized in alginate-polyvinyl alcohol beads

Garay-Flores

Segura-Ceniceros

León-Gámez

et al. 2014

J. Gen. Appl. Microbiol.

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CAT) by submerged fermentation of the free and immobilized xerophytic fungus Aspergillus niger under equal conditions was compared. To immobilize fungal spores, entrapment in PVA/alginate beads treated with NaNO 3 /CaCl 2 was performed. The yield of immobilization in the beads with a diameter less than 1mm was equal to 100%. Fungus growth and substrate consumption were evaluated in both fermentation systems, demonstrating the lag-period presence in the case of the first cycle of immobilized fungus use. The enzyme production by immobilized fungus reuse was carried out. In these cases, greater enzymatic GOX activity was detected, while CAT activity decreased. SEM micrographs for the beads with immobilized fungus applied in the first and second fermentation cycles were obtained, presenting fungus spreading inside the sphere, spore presence and branching hyphae. Immobilization of A. niger on PVA/alginate beads is effective for GOX and CAT production at least on 2 3 repeated fermentative cycles. Thus, immobilization enables repeated use of microbial cells.

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Comparison of Two Methods for Assaying Reducing Sugars in the Determination of Carbohydrase Activities

Cited by 208 publications

References 23 publications

Asymmetric dumbbell-shaped silver nanoparticles and spherical gold nanoparticles green-synthesized by mangosteen (<em>Garcinia mangostana</em>) pericarp waste extracts

Asymmetric dumbbell-shaped silver nanoparticles and spherical gold nanoparticles green-synthesized by mangosteen (<em>Garcinia mangostana</em>) pericarp waste extracts

Hypolipidemic Effect of the Autoclaved Extract Prepared from Pea (<i>Pisum sativum</i> L.) Pods In Vivo and In Vitro

Production of glucose oxidase and catalase by Aspergillus niger free and immobilized in alginate-polyvinyl alcohol beads

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