Comparison of two mononuclear cell program settings on two apheresis devices intended to collect high yields of CD14+ and CD3+ cells

Abstract

Compared to the program modifications with lower centrifuge velocities the standard MNC programs were significantly more efficient regarding CD14+, CD3+, and CD16+ CD56+ cells. Enhanced centrifuge speed and inlet blood flow rate in MNC programs resulted in higher, similar composed MNC concentrations of the products.

Low-Volume Leukapheresis in Non-Cytokine-Stimulated Donors for the Collection of Mononuclear Cells

“…At the same time, the product Hct within a range from 1.2 to 6.0% had no influence on the CE2 values and the cell yield of any cell population or on the contamination with residual cells. In contrast to our findings, Strasser et al [12], who had compared the collection result of leukapheresis with high-component Hct to procedures with low-component Hct, found significantly higher CD14+ cell yields with a simultaneous higher collection efficiency of MNCs in components with higher Hct. The WBC yield and the PLT contamination had also increased in those components, but without reaching significance.…”

“…Notwithstanding the above, the CMNC program remains easier to monitor and can be controlled more precisely than the MNC program. In addition, previous studies focused mainly on the differences in leukapheresis procedures and their performance [11,12]. Only a few publications included the short-term impact of the procedure on the donor [5,13].…”

Short‐term liquid storage of CD14+ monocytes, CD11c+, and CD123+ precursor dendritic cells produced by leukocytapheresis

“…The rate of the plasma pump was set manually by visual inspection of the collection line to target a hematocrit level of 1 to 2 percent. Anticoagulation was performed with solution acid citrate dextrose, formula A (ACD‐A, Fresenius HemoCare GmbH) and adjusted to a basic ratio of ACD‐A to whole blood of 1:11, by use of an inlet blood flow rate of 60 mL per minute 15 …”

“…The ratio of ACD‐A (Fresenius HemoCare GmbH) to blood was 1:11. The cell‐to‐plasma interface was adjusted to a constant ratio of 7:1 and automatically controlled by the device, whereas the spillover and buffy‐coat volume was calibrated by the operator 15 . Procedures of both apheresis devices were conducted following the manufacturer's instructions.…”

Automated CD14+ monocyte collection with the autoMNC program of the COM.TEC cell separator

“…We found an excellent collection efficiency (CE) of CD14+ monocytes 1 . Leukapheresis units usually contain between 1 × 10 9 and 3 × 10 9 monocytes, which depends on the processing time and the apheresis device used 1‐5 . The standard MNC program of the COM.TEC cell separator, however, also collected large yields of residual platelets (PLTs) and red blood cells (RBCs) 1 .…”