Comparison of two techniques for a comprehensive gut histopathological analysis: Swiss Roll versus Intestine Strips

Silva, Airton Pereira e; Lourenço, André Luiz; Marmello, Bárbara Oliveira; Bitteti, Monique; Teixeira, Gerlinde Agate Platais Brasil

doi:10.1016/j.yexmp.2019.104302

Cited by 9 publications

(11 citation statements)

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“…We believe that our results will contribute to the works of researchers that are involved in the intestinal histological evaluations of broilers. There are several studies comparing different forms of intestinal tissue sampling for histological evaluation in rodents (Silva et al, 2019;Williams et al, 2016), with comparatively few data for broilers.…”

Section: Resultsmentioning

confidence: 99%

“…On the other section, the hemicylindrical sectional method was considered the not evaluated (Gava et al, 2015). In addition, in rodents, intestin wider histological evaluation (Silva et al, 2019), while the gut b villi and crypt measurements (Williams et al, 2016). To the best evaluation of Swiss roll sampling method in broilers' intestine.…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, in a study using intes section, the hemicylindrical sectional method was considered the best, but the Swiss roll met not evaluated (Gava et al, 2015). In addition, in rodents, intestinal strips were considered mo wider histological evaluation (Silva et al, 2019), while the gut bundling method was conside villi and crypt measurements (Williams et al, 2016). To the best of the authors´ knowledge, t evaluation of Swiss roll sampling method in broilers' intestine.…”

Section: Resultsmentioning

confidence: 99%

“…There are numerous studies in rodents to evaluate different sampling methods (Bialkowska;Ghaleb, Nandan, & Yang, 2016;Moolenbeek & Ruitenberg, 1981;Silva, Lourenço, Marmello, Bitteti, & Teixeira, 2019;Williams, Duckworth, Vowell, Burkitt, & Pritchard, 2016). However, similar evaluations in broilers are scarce; to the best of the authors´ knowledge this is the first study to evaluate the intestine of broilers with the Swiss roll sampling technique.…”

Section: Introductionmentioning

confidence: 99%

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Histological evaluation of the intestine of broiler chickens: comparison of three sampling methods

Souza

Cicero

Menck-Costa

et al. 2021

Semina: Ciênc. Agrár.

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After the growth-promoting antibiotics prohibition, intestinal health became an increasing concern worldwide in poultry farming. The intestinal histological evaluation is an inexpensive technique that brings relevant information, but in poultry, the immediate process of intestinal post-mortem autolysis interferes directly on the samples quality for histological analysis hindering a precise diagnosis. This study aimed to standardize a technique for broilers’ intestines sample collection and fixation for histological analysis. Seven broiler chickens received a standard diet until 23 days of age when they were euthanized. Fragments of duodenum, jejunum, and ileum were collected using three methods: intestine strips, transverse section, and Swiss roll and posteriorly fixed in 10% buffered formalin and bouin solution. Tissue samples were submitted for histological (number of villi and viable villi per field) and morphometrical (villi height, crypt depth and villi:crypt ratio) evaluations and the results analyzed statistically. A significant high number of villi and viable villi per field in all regions was observed in the Swiss roll method. In the duodenum (p= 0.0066) and jejunum (p= 0.0058) an interaction between the Swiss roll method and the fixative buffered formalin was observed in the viable and number of villi per field, respectively. Regarding the morphometrical analysis significant differences were observed, in the jejunum villi height sampling by the methods Swiss roll (1,157.66 ± 148.25 μm, p= 0.0015) that showed the highest mean. Deeper crypt depths were observed in the jejunum (156.59 ± 15.68 μm, p= 0.0002) and ileum (131.13 ± 15.01 μm, p= 0.0006) collect by the Swiss roll method. An interaction between the bouin fixative was also observed in the jejunum (p= 0.0223) for this variable. Duodenum sampling by transversal section (12.68 ± 1.45 μm, p= 0.0076) was the only segment that had a significant difference for villi:crypt ratio, showing the highest mean. It can be concluded that the Swiss roll technique was the best method for morphometrical evaluation of the chickens’ intestines, since the highest counts of villi per field and viable villi per field were obtained, while buffered formalin was considered as the best fixative.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Histological evaluation of the intestine of broiler chickens: comparison of three sampling methods

Souza

Cicero

Menck-Costa

et al. 2021

Semina: Ciênc. Agrár.

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“…Histological investigations of the murine GIT are mostly performed using thin slices of tissue fragments or conventional Swiss rolls (82)(83)(84) or only focused on particular areas of the intestinal tract (52,63) and thus, inherently underrepresent its three-dimensional complexity. 3D-Swiss Rolls allowed us to clear and image the small intestine and colon in full length and circumference at cellular resolution without affecting its microanatomy.…”

Section: Discussionmentioning

confidence: 99%

ROCKETS - a novel one-for-all toolbox for light sheet microscopy in drug discovery

Mueller

Dobosz

O’Brien

et al. 2022

Preprint

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Advancing novel immunotherapy strategies requires refined tools in preclinical research to thoroughly assess drug targets, biodistribution, safety, and efficacy. Light sheet fluorescence microscopy (LSFM) offers unprecedented fast volumetric ex vivo imaging of large tissue samples in high resolution. Yet, to date laborious and unstandardized tissue processing procedures have limited throughput and broader applications in immunological research. Therefore, we have developed a simple and harmonized protocol for processing, clearing and imaging of all mouse organs and even entire mouse bodies. Applying this Rapid Optical Clearing Kit for Enhanced Tissue Scanning (ROCKETS) in combination with LSFM allowed us to comprehensively study the in vivo biodistribution of an antibody targeting Epithelial Cell Adhesion Molecule (EpCAM) in 3D. Quantitative high-resolution scans of whole organs did not only reveal known EpCAM expression patterns but, importantly, uncovered several new EpCAM-binding sites. We identified choroid plexi in the brain and duodenal papillae as unexpected locations of high EpCAM-expression. These tissue locations may be considered as particularly sensitive sites due their importance for liquor production or as critical junctions draining bile and digestive pancreatic enzymes into the small bowel, respectively. These newly gained insights appear highly relevant for clinical translation of EpCAM-addressing immunotherapies. Thus, ROCKETS in combination with LSFM may help to set new standards for preclinical evaluation of immunotherapeutic strategies. Conclusively, we propose ROCKETS as an ideal platform for a broader application of LSFM in immunological research optimally suited for quantitative co-localization studies of immunotherapeutic drugs and defined cell populations in the microanatomical context of organs or even whole mice.

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The Gastrointestinal Tract

Ward¹,

Treuting²,

Washington³

2021

Pathology of Genetically Engineered and Other Mutant Mice

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Comparison of two techniques for a comprehensive gut histopathological analysis: Swiss Roll versus Intestine Strips

Cited by 9 publications

References 50 publications

Histological evaluation of the intestine of broiler chickens: comparison of three sampling methods

Histological evaluation of the intestine of broiler chickens: comparison of three sampling methods

ROCKETS - a novel one-for-all toolbox for light sheet microscopy in drug discovery

The Gastrointestinal Tract

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