Comparison of Unsatisfactory Samples from Conventional Smear versus Liquid-Based Cytology in Uterine Cervical Cancer Screening Test

Jeong, Hoiseon; Hong, Sung Ran; Chae, Seoung-Wan; Jin, So-Young; Yoon, Hye Kyoung; Lee, Juhie; Kim, Min Jung; Ha, Sook Tai; Kim, Sung Nam; Park, Eun-Jung; Jung, Jong Jae; Sung, Sun Hee; Lim, Sung‐Chul

doi:10.4132/jptm.2017.03.17

Cited by 24 publications

(26 citation statements)

References 21 publications

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“…Although conventional cytology has been replaced by LBC testing as the primary test method in the majority of developed countries within the last 10 to 15 years, it remains an option for women in China. Numerous earlier studies have compared the performance of LBC with conventional cytology and demonstrated the advantages of LBC, including increased sensitivity, lower rates of unsatisfactory slides, and the ability to perform HPV DNA testing on the same specimen . However, others also addressed the issue of decreased specificity with LBC and the cost of follow‐up .…”

Section: Discussionmentioning

confidence: 99%

Follow‐up with histopathology and HPV testing on LSIL cytology in China’s largest academic woman’s hospital

Tao

Zhang

et al. 2019

Cancer Cytopathology

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Background Cervical cancer remains a major health problem in China. To provide baseline data for establishing the appropriate screening strategy, the authors investigated the histopathologic follow‐up and human papillomavirus (HPV) testing results of low‐grade squamous intraepithelial lesion (LSIL) cytology in the Chinese Largest Women Hospital. Methods Women with LSIL cytology, human papillomavirus (HPV) testing, and immediate histopathologic follow‐up between 2011 and 2016 were analyzed. Results The frequency of LSIL among 1,095,022 Papanicolaou (Pap) tests was 1.4%, and was significantly higher in cases using liquid‐based cytology (LBC; 1.5%‐1.6%) compared with conventional Pap smears (CPS; 0.5%). The CPS method had performance for predicting cervical intraepithelial neoplasia (CIN) that was comparable to that of LBC (75% vs 69.1% and 71.9%). Among 8079 LSIL cases with an immediate histopathologic diagnosis, CIN‐2/3 or higher (CIN‐2/3+) was found in 11.9% of cases and carcinoma in 0.3% of cases. A total of 158 patients (15.7%) with HPV‐positive LSIL had a diagnosis of CIN‐2/3 compared with 18 patients (5.5%) with HPV‐negative LSIL. In postmenopausal women with LSIL cytology, the detection rate for CIN‐2/3 in HPV‐negative patients was significant lower than that in HPV‐positive patients. Furthermore, HPV type 16 (HPV‐16) was the most common genotype noted in patients with CIN‐2+ lesions, whereas HPV‐18 was the most common in patients with CIN‐1. Conclusions Although its reporting rate is lower than that of LBC, CPS is useful due to its low cost and comparable predictive value. HPV testing is helpful for stratifying postmenopausal women with LSIL into lower and higher risk groups. HPV‐16 is the most commonly identified genotype in CIN‐2+ lesions diagnosed in Chinese women with LSIL cytology, whereas HPV‐18 is the most commonly identified genotype in patients with CIN‐1 lesions.

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Section: Discussionmentioning

confidence: 99%

Follow‐up with histopathology and HPV testing on LSIL cytology in China’s largest academic woman’s hospital

Tao

Zhang

et al. 2019

Cancer Cytopathology

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“…Recently, liquid‐based cytology (LBC) has become a useful cytopathological method for diagnosis. In the early 2000s, the LBC method began to be used widely, especially in gynecological malignancies . LBC has advantages in that uniform specimens can be prepared, drying is avoided, and there is less sample loss.…”

Section: Introductionmentioning

confidence: 99%

“…LBC has advantages in that uniform specimens can be prepared, drying is avoided, and there is less sample loss. Consequently, fewer unsatisfactory specimens are produced . Therefore, the use of LBC specimens for the clinical diagnosis of respiratory malignancies also is expected …”

Section: Introductionmentioning

confidence: 99%

“…In the early 2000s, the LBC method began to be used widely, especially in gynecological malignancies. [1][2][3][4][5][6][7][8][9][10][11] LBC has advantages in that uniform specimens can be prepared, drying is avoided, and there is less sample loss. Consequently, fewer unsatisfactory specimens are produced.…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, fewer unsatisfactory specimens are produced. [5][6][7][8][9][10] Therefore, the use of LBC specimens for the clinical diagnosis of respiratory malignancies also is expected. 12 Lung cancer has many histological types, and a histological diagnosis of the disease is very important for informing therapeutic strategies.…”

Section: Introductionmentioning

confidence: 99%

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Reducing DNA damage by formaldehyde in liquid‐based cytology preservation solutions to enable the molecular testing of lung cancer specimens

Matsuo

Yoshida

Yamashita

et al. 2018

Cancer Cytopathology

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Background Liquid‐based cytology (LBC) is a useful cytopathological method, and LBC lung adenocarcinoma specimens may be used for genetic analysis in the near future. In the current study, the authors determined whether LBC specimens can be used for epidermal growth factor receptor (EGFR) mutation analysis in human lung adenocarcinoma cell lines. Methods Genomic DNA was extracted from 3 lung adenocarcinoma cell lines that were fixed in LBC preservation solution using 2 protocols (one for cultured cells and one for tissues) of a DNA extraction kit. Different fixation times were tested for each protocol: 30 minutes, 1 hour, and 1 to 9 days. As controls, cells also were fixed in 10% formalin or 95% ethanol. The authors investigated the effect of fixation time on DNA fragmentation, polymerase chain reaction (PCR) amplification, and EGFR mutation detection. Results The DNA yield of LBC specimens tended to decrease depending on fixation time. When using the DNA extraction protocol for tissues, PCR amplification was successful after 9 days of fixation, although extracted genomic DNA that was fixed for >1 hour demonstrated fragmentation. Mutation analyses using the Cycleave PCR method were successful after 7 days of fixation. The DNA extraction protocol for tissues was appropriate for lung adenocarcinoma cell lines that were stored for >1 day in a preservative solution. The results of the current study demonstrated that EGFR mutations can be detected on day 7 using lung adenocarcinoma cell lines fixed in CytoRich Red preservative. Conclusions When LBC specimens are used for targeted molecular genetic testing, the appropriate preservative solution and extraction protocol first should be determined.

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Poor Performance of Applicator Tampon‐Based Self‐Collection for Liquid‐Based Cytology Among Women Attending a Tertiary Hospital in South Africa

Tiiti,

Nkwinika,

Mashishi

et al. 2024

Diagnostic Cytopathology

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BackgroundThe South African Cervical Cancer Prevention and Control Policy was updated in June 2017, recommending liquid‐based cytology (LBC) as the preferred screening method and the investigation of self‐sampling for cervical cancer screening.AimTo compare the performance of the Self Collection Cervical Health Screening Kit [SelfCerv (applicator tampon)] to the Cervex‐Brush Combi for cytology screening. The study further aimed to compare high‐risk (hr‐) human papillomavirus (HPV) and LBC test results from both methods.MethodsThe study included 446 paired samples, comprising self‐collected (SelfCerv) and healthcare provider‐collected (Cervex‐Brush Combi) samples from women aged ≥ 18 years attending gynaecology outpatient clinics at a tertiary hospital in Pretoria, South Africa. LBC slides were prepared using the ThinPrep 5000 processor and manually stained with Hematoxylin and Eosin (H&E). Detection of 14 hr‐HPV types was performed using the Abbott RealTime HR‐HPV assay. Statistical analyses were performed using STATA version 17.0 (Stata Corp., College Station, Texas, USA).ResultsA statistically significant difference in cervical cytology detection between the two methods was observed (p = 0.0025). The Cervex‐Brush Combi was more effective in collecting endocervical cells (73.4%; 95% CI: 69.0–77.9) compared to the SelfCerv applicator tampon (7.3%; 95% CI: 4.7–9.9); (p < 0.001). Cytological abnormalities were detected in 65.4% (136/208) of participants who tested positive for hr‐HPV by healthcare provider sampling compared to 40.8% (84/206) by self‐sampling. A fair agreement (κ: 0.35) with a concordance rate of 96.2% (95% CI: 94.4–98.0) was observed for specimen adequacy and diagnosis parameters [κ: 0.34, with a concordance rate of 67.7% (95% CI: 63.2–72.1)] between the two methods.ConclusionThe findings of this study do not support the implementation of applicator tampon‐based self‐collection as a method for cytology‐based cervical cancer screening.

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Comparison of Unsatisfactory Samples from Conventional Smear versus Liquid-Based Cytology in Uterine Cervical Cancer Screening Test

Cited by 24 publications

References 21 publications

Follow‐up with histopathology and HPV testing on LSIL cytology in China’s largest academic woman’s hospital

Follow‐up with histopathology and HPV testing on LSIL cytology in China’s largest academic woman’s hospital

Reducing DNA damage by formaldehyde in liquid‐based cytology preservation solutions to enable the molecular testing of lung cancer specimens

Poor Performance of Applicator Tampon‐Based Self‐Collection for Liquid‐Based Cytology Among Women Attending a Tertiary Hospital in South Africa

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