Comparison of visual vs. automated detection of lipemic, icteric and hemolyzed specimens: can we rely on a human eye?

Šimundić, Ana-Maria; Nikolac, Nora; Ivankovic, Valentina; Ferenec-Ružić, Dragica; Magdic, Bojana; Kvaternik, Marina; Topić, Elizabeta

doi:10.1515/cclm.2009.306

Cited by 91 publications

(55 citation statements)

References 20 publications

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“…Lippi et al compared the efficiency of different analyzers including Olympus AU680 to evaluate the hemolyzed samples and found that results were satisfactory [12]. In another study, Simundic et al assessed the comparability of automated spectrophotometric detection by Olympus AU 2700 and visual inspection of hemolyzed samples and showed a comparable rate of detection for hemolyzed samples [13]. In the present study, Hb levels in the samples detected automatically with LIH index of Olympus AU 640 were very close to manually measured and calculated Hb values (Table 2 and 3).…”

Section: Discussionmentioning

confidence: 99%

Effects of hemolysis on the assays of serum CK, CK-MB activities and CK-MB (MASS), troponin and myoglobin measurments

Özcan¹,

Karakas²,

Yücel³

2012

Turk J Bioch

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Aim: The aim of the study was to investigate the effects of in vitro hemolysis on serum creatine kinase and creatine kinase MB isoenzyme activities with creatine kinase MB mass, troponin I and myoglobin measurements. Materials and methods:We prepared serum pools having analyte concentrations at normal and pathological values. Hemolysate was added into serum pools to obtain aliquotes with final hemoglobin concentrations of 21, 10.5, 5.25, 2.625, 1.312, 0.656, 0.328, 0.164, 0.08 and 0.041 g/L. Creatine kinase (by N-acetylcysteine-activated IFCC method) and creatine kinase MB activities (by immunoinhibition method) were measured in these pools. Creatine kinase MB (mass), troponin I and myoglobin concentrations were measured by chemiluminescence method. Mean percent changes were calculated and graphed as interferographs. Results: It was found that the positive interference due to hemolysis began to exceed the limit of 10% at lower Hb concentration for CK-MB activity than CK activity. When reference change value were considered, the critical effect of hemolysis began at higher hemoglobin concentrations. Hemolysis did not affect creatine kinase MB mass, troponin and myoglobin measurements when the limit of 10% change was considered. Conclusions: CK-MB activity is affected more profoundly than CK activity by hemolysis.

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Section: Discussionmentioning

confidence: 99%

Effects of hemolysis on the assays of serum CK, CK-MB activities and CK-MB (MASS), troponin and myoglobin measurments

Özcan¹,

Karakas²,

Yücel³

2012

Turk J Bioch

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“…Besides being highly subjective and arbitrary, this approach is unsuitable when a large number of samples have to be inspected [33] . Analyzers may detect the degree of lipemia by measuring sample specter on several wavelengths.…”

Section: Lipemiamentioning

confidence: 99%

Preanalytical quality improvement: in quality we trust

Lippi

Becan-McBride

Behúlová

et al. 2012

Clinical Chemistry and Laboratory Medicine (CCLM)

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191

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Total quality in laboratory medicine should be defined as the guarantee that each activity throughout the total testing process is correctly performed, providing valuable medical decision-making and effective patient care. In the past decades, a 10-fold reduction in the analytical error rate has been achieved thanks to improvements in both reliability and standardization of analytical techniques, reagents, and instrumentation. Notable advances in information technology, quality control and quality assurance methods have also assured a valuable contribution for reducing diagnostic errors. Nevertheless, several lines of evidence still suggest that most errors in laboratory diagnostics fall outside the analytical phase, and the pre-and postanalytical steps have been found to be much more vulnerable. This collective paper, which is the logical continuum of the former already published in this journal 2 years ago, provides additional contribution to risk management in the preanalytical phase and is a synopsis of the lectures of the 2nd European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)-Becton Dickinson (BD) European Conference on Preanalytical Phase meeting entitled " Preanalytical quality improvement: in quality we trust " (Zagreb, Croatia, 1 -2 March 2013). The leading topics that will be discussed include quality indicators for preanalytical phase, phlebotomy practices for collection of blood gas analysis and pediatric samples, lipemia and blood collection tube interferences, preanalytical requirements of urinalysis, molecular biology hemostasis and platelet testing, as well as indications on best practices for safe blood collection. Auditing of the preanalytical phase by ISO assessors and external quality assessment for preanalytical phase are also discussed.

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“…Los índices pueden ser usados para detectar la interferencia generando unas tablas de tolerancia, con unos índices de decisión que nos indican la presencia de interferencia (4,11,13,14,17) .…”

Section: Discussionunclassified

Interferencia en las determinaciones de 24 constituyentes bioquímicos en el autoanalizador ADVIA 1800, causada por adición in vitro de emulsión comercial de nutrición parenteral a un pool de sueros

Orejón

2016

An Fac med

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ResumenIntroducción. Las interferencias analíticas por turbidez pueden causar errores clínicamente significativos en los resultados de una magnitud biológica. Objetivos. Conocer y cuantificar la posible interferencia producida por la turbidez (lipemia) en la medición rutinaria de 24 constituyentes bioquímicos, empleando para ello el criterio de interferencia clínicamente relevante cuando se supera el máximo error sistemático deseable. Diseño. Estudio experimental con pre y pos-prueba. Institución. Hospital Edgardo Rebagliati Martins, Lima, Perú. Material biológico. Pool de sueros proveniente de muestras procesadas en el laboratorio central del hospital. Intervenciones. Se añadieron cantidades crecientes de una emulsión lipídica comercial de nutrición parenteral a siete diferentes alícuotas de una mezcla de sueros y se determinó en ellas, por triplicado, la influencia del interferente en los 24 constituyentes. Para lo cual se ha seguido el protocolo de la Sociedad Española de Química Clínica. Principales medidas de resultados. Porcentaje relativo de desviación de la concentración del constituyente por influencia de la turbidez, con respecto a una muestra sin interferente. Resultados. Los constituyentes: glucosa, creatinina, potasio, colesterol total, HDL colesterol, fosfatasa alcalina, gammaglutamil transferasa, amilasa y lipasa, no presentaron interferencia en todas las alícuotas con cantidades crecientes del interferente empleado en el ensayo. Se observó interferencia para urea, ácido úrico, proteínas totales, albúmina, bilirrubina total, LDL colesterol, transferrina, fósforo, hierro, láctico deshidrogenasa, aspartato aminotransferasa, alanino aminotransferasa, creatinfosfocinasa, calcio y magnesio. Conclusiones. De los 24 constituyentes estudiados, 15 presentaron interferencia clínicamente significativa. Se recomienda que cada laboratorio investigue los efectos de dicha interferencia empleando sus propios métodos, reactivos o instrumentos. Palabras clave. Turbidez por Lipemia, interferencia; Error Sistemático Deseable. Abstract Introduction. The analytical interference due to turbidity may cause clinically significant errors in the results of biological magnitude. Objectives. To identify and quantify turbidity (lipemia) possible interference in the routine measurement of 24 biochemical constituents using clinically relevant interference criterion when the maximum desirable systematic error is exceeded. Design. Experimental study with pre and post-test. Setting. Hospital Edgardo Rebagliati Martins, Lima, Peru. Biological material: Pooled sera from samples processed at the central laboratory of the hospital. Interventions. Increasing amounts of a parenteral nutrition commercial lipid emulsion were added to seven different aliquots of a pool of sera, and the influence of interference in 24 constituents was determined by triplicate. The Spanish Society of Clinical Chemistry guideline was followed. Main outcome measures. Lipemia-influenced constituent concentration relative percent deviation, compared with a sample...

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Comparison of visual vs. automated detection of lipemic, icteric and hemolyzed specimens: can we rely on a human eye?

Cited by 91 publications

References 20 publications

Effects of hemolysis on the assays of serum CK, CK-MB activities and CK-MB (MASS), troponin and myoglobin measurments

Effects of hemolysis on the assays of serum CK, CK-MB activities and CK-MB (MASS), troponin and myoglobin measurments

Preanalytical quality improvement: in quality we trust

Interferencia en las determinaciones de 24 constituyentes bioquímicos en el autoanalizador ADVIA 1800, causada por adición in vitro de emulsión comercial de nutrición parenteral a un pool de sueros

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