Comparison on hypoglycemic and antioxidant activities of the fresh and dried Portulaca oleracea L. in insulin-resistant HepG2 cells and streptozotocin-induced C57BL/6J diabetic mice

Gu, Junfei; Zheng, Zhi; Yuan, Jiarui; Zhao, Bingjie; Wang, Chunfei; Zhang, Li; Xu, Qingyu; Yin, Guowen; Feng, Liang; Jia, Xiao‐Bin

doi:10.1016/j.jep.2014.12.002

Cited by 61 publications

(34 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The crude polysaccharide extract of this plant was also found to lower blood glucose and modulate the metabolism of blood lipids and glucose in alloxan- [17] as well as in STZ-induced diabetic mice [18], whilst decreasing the levels of total cholesterol, triglycerides, and fasting blood glucose in type II diabetic mice [19]. Gu et al [20] compared the hypoglycemic and antioxidant activities of the fresh and dried P. oleracea L. in insulin-resistant HepG2 cells and streptozotocin-induced C57BL/6 J diabetic mice. Their results indicated that both fresh and dried P. oleracea extract possessed antidiabetic and antioxidant activities, besides stronger activity was observed in the fresh herb [20].…”

Section: Introductionmentioning

confidence: 99%

“…Gu et al [20] compared the hypoglycemic and antioxidant activities of the fresh and dried P. oleracea L. in insulin-resistant HepG2 cells and streptozotocin-induced C57BL/6 J diabetic mice. Their results indicated that both fresh and dried P. oleracea extract possessed antidiabetic and antioxidant activities, besides stronger activity was observed in the fresh herb [20]. …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hypoglycemic and pancreatic protective effects of Portulaca oleracea extract in alloxan induced diabetic rats

Ramadan

Schaalan

Tolba

2017

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundDiabetes is a major public health concern. In spite of continuous new drug development to treat diabetes, herbal remedies remain a potential adjunct therapy to maintain better glycemic control while also imparting few side-effects. Portulaca oleracea has been traditionally used to manage several diseases due to the anti-oxidant and anti-atherogenic effects it imparts. To better understand the mechanisms associated with potential protective effect of P. oleracea extract against diabetes, alloxan-induced diabetic rats were used in this study.MethodsForty Wistar rats (male, 7–8-wk-old, 140–160 g) were divided into four groups (n = 10/group): Group I (control), Group II (P. oleracea-treated; gavaged with P. oleracea extract daily [at 250 mg/kg] for 4 weeks), Group III (diabetic control; daily IP injection of alloxan [at 75 mg/kg] for 5 days) and Group IV (P. oleracea-pre-treated diabetic; gavaged with P. oleracea extract daily [at 250 mg/kg] for 4 weeks and then daily IP injection of alloxan [at 75 mg/kg] for 5 days). Body weight, food consumption, blood (serum) levels of glucose, C peptide, Hb A1C, insulin, tumor necrosis factor (TNF)-α and interleukin (IL)-6 were determined for all groups.ResultsThe results indicated that while Hb A1C, serum levels of glucose, TNF-α and IL-6 were all significantly decreased in the P. oleracea-pre-treated diabetic rats, these hosts also had significant increases in C peptide and insulin compared to levels in the counterpart diabetic rats. These results were confirmed by the histopathological assessments which showed marked improvement of the destructive effect on pancreatic islet cells induced by alloxan.ConclusionP. oleracea extract is a general tissue protective and regeneartive agent, as evidenced by increasing β-cell mass and therefore improved the glucose metabolism. Thus, stimulation of Portulaca oleracea signaling in β- cells may be a novel therapeutic strategy for diabetes prevention.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hypoglycemic and pancreatic protective effects of Portulaca oleracea extract in alloxan induced diabetic rats

Ramadan

Schaalan

Tolba

2017

BMC Complement Altern Med

View full text Add to dashboard Cite

show abstract

“…In brief, the HepG2 cells were seeded on 96‐well plates and cultured for 24 hr. The insulin‐resistant cell model was established according to the previous reference method (Gu et al, ). Thereafter, HepG2 cells were treated with 10 −9 mM insulin and different concentrations of WHPs (10, 20, 50, 80, and 100 μg/mL) or 100 μg/mL Met in RPMI‐1640 medium containing 1.0% FBS, respectively.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the antidiabetic activity of hydrolyzed peptides derived fromJuglans mandshuricaMaxim. fruits in insulin-resistant HepG2 cells and type 2 diabetic mice

et al. 2018

J Food Biochem

View full text Add to dashboard Cite

Bioactive peptides derived from plant proteins are promising sources for the development safe hypoglycemics. The present study was designed to investigate the antidiabetic activity of walnut hydrolyzed peptides (WHPs) obtained from the fruit proteins of Juglans mandshurica Maxim. In vitro results showed that WHPs with medium molecular weights (3-10 kDa) exhibited the highest a-glucosidase inhibitory rate (61.73%) and significantly increased extracellular glucose consumption in insulin-resistant HepG2 cells. In mice with streptozotocin-induced type 2 diabetes, WHPs with medium molecular weights reduced fasting blood glucose levels by 64.82% and increased insulin secretion by 23.71% and liver glucokinase and glycogen levels by 69.54 and 76.19%, respectively. Furthermore, an analysis of serum lipid profiles showed that WHPs significantly decreased serum total cholesterol, triglyceride, and low-density lipoprotein cholesterol levels. Our results indicate the antidiabetic potential of WHPs and provide evidence to support the application of WHPs in the treatment of diabetes. Practical applicationsJuglans mandshurica Maxim., commonly known as the Manchurian walnut, is a member of the Juglandaceae plant family. Its fruit is extensively consumed in northeast China and is used in the production of dairy and bakery products, candy, and cooking oil owing to its high protein content (about 30%), abundance of essential amino acids, and easy digestibility. Hence, there has been an immense increase, up to a million hectare, in the area under Manchurian walnut cultivation. The results of the present study indicated that WHPs showed significant antidiabetic activity by improving a-glucosidase activity, glucose metabolism, insulin secretion, and liver glucokinase and glycogen levels as well as by decreasing the fasting blood glucose level. These findings have identified a green source of novel antidiabetic peptides that can either be used as a functional food ingredient in the management of hyperglycemia or developed as a drug for the treatment of type 2 diabetes.

show abstract

“…Besides, T2DM also causes abnormalities in the metabolism of lipids and lipoproteins (Liu et al, 2013a). STZ, an antibiotic produced by Streptomyces achromogenes, is widely used to induce diabetes mellitus in experimental animals due to its toxic effects on pancreatic b-cells (Gu et al, 2015). In addition, the cytotoxic action of STZ is associated with the oxidative damage caused by ROS (Li et al, 2006).…”

Section: Methylation Analysis Of Yz-2mentioning

confidence: 99%

Physicochemical properties and antidiabetic effects of a polysaccharide obtained from Polygonatum odoratum

Jiang

Sun

et al. 2018

Int J of Food Sci Tech

View full text Add to dashboard Cite

Summary In this study, we isolated a novel polysaccharide (YZ‐2) (molecular weight: 5345.3 Da) from Polygonatum odoratum (Mill.) Druce and investigated its physicochemical properties and antidiabetic effects in the type 2 diabetic mellitus (T2DM) mice induced by a low dose of streptozotocin coupled with a high‐fat diet. The results showed that YZ‐2 had no triple‐helix conformation and was mainly composed of α‐mannopyranose, α‐glucopyranose and α‐galactopyranose residues. Moreover, YZ‐2 could significantly decrease the fasting blood glucose (Glc) level (P < 0.01), the serum TC, TG and LDL‐c concentrations (P < 0.05), as well as the fasting insulin level and improve the Glc tolerance capacity in the T2DM mice. Additionally, YZ‐2 could protect livers and pancreas by remarkably increasing SOD and CAT activities and GSH content (P < 0.05) in the liver of T2DM mice. Collectively, these results suggested that YZ‐2 could be a promising functional food supplement for T2DM treatment.

show abstract

Comparison on hypoglycemic and antioxidant activities of the fresh and dried Portulaca oleracea L. in insulin-resistant HepG2 cells and streptozotocin-induced C57BL/6J diabetic mice

Cited by 61 publications

References 45 publications

Hypoglycemic and pancreatic protective effects of Portulaca oleracea extract in alloxan induced diabetic rats

Hypoglycemic and pancreatic protective effects of Portulaca oleracea extract in alloxan induced diabetic rats

Evaluation of the antidiabetic activity of hydrolyzed peptides derived fromJuglans mandshuricaMaxim. fruits in insulin-resistant HepG2 cells and type 2 diabetic mice

Physicochemical properties and antidiabetic effects of a polysaccharide obtained from Polygonatum odoratum

Contact Info

Product

Resources

About