Comparison study of the effect of alkyl-modified and unmodified PAMAM and PPI dendrimers on solubility and antitumor activity of crocetin

Soltani, Fatemeh; Ramezani, Mohammad; Farzad, Sara Amel; Mokhtarzadeh, Ahad; Hashemi, Maryam

doi:10.1080/21691401.2016.1236805

Cited by 20 publications

(10 citation statements)

References 35 publications

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“…Then, the efficiency of the synthetized vectors for the delivery of TRAIL plasmid was evaluated in mouse colon cancer cell line, in vitro and in vivo. Mouse TRAIL R2, also called DR5, TRICK 2, TNFRSF10B, and MKDR5 shares 43 and 49% sequence homology with human TRAIL-R1 and TRAIL-R2, respectively [24]. The ability of dendrimers to condense DNA is an essential requirement for successful gene delivery.…”

Section: Discussionmentioning

confidence: 99%

“…The effect of synthetized vector containing TRAIL plasmid on induction of apoptosis was evaluated as described previously [24,25]. Briefly, C26 cells were seeded in 24-well plates at a density of 4 Â 10 4 cells/well and incubated for 24 h. Polyplexes with C/P ratio 2 and 4 were prepared and added into each well (equivalent of 800 ng TRAIL plasmid/well).…”

Section: Sub-g1 Apoptosis Assay Using Flow Cytometrymentioning

confidence: 99%

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Modified PAMAM vehicles for effective TRAIL gene delivery to colon adenocarcinoma:in vitroandin vivoevaluation

Pishavar

Attaranzadeh

Alibolandi

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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TRAIL (tumour necrosis factor-related apoptosis-inducing ligand) gene therapy is considered as one of the promising approaches for cancer treatment. Polyamidoamine (PAMAM) is one of the most extensively applied polymeric vector in gene delivery. In the current study, PAMAM (G4 and G5) dendrimers were modified with alkyl-carboxylate chain, PEG and cholesteryl chloroformate in order to enhance transfection efficiency through overcoming extracellular and intracellular barriers while reducing PAMAM cytotoxicity. Gene delivery efficiency of synthetized vectors was evaluated by both GFP (green fluorescent protein) reporter gene and TRAIL plasmid in colon cancer cells, in vitro and in vivo. The obtained results demonstrated that PAMAM G4-alkyl-PEG (3%)-Chol (5%)-TRAIL complexes at C/P ratio 4 could significantly increase cell death (29.45%) in comparison with unmodified PAMAM vector (15.5%). Moreover, in vivo study in C26 tumor-bearing BALB/c mice suggested that the prepared non-toxic safe vector could inhibit the tumor growth. This study represented the potent vehicle based on cholesterol-grafted PAMAM dendrimers with alkyl-PEG modification for efficient gene delivery in vitro and in vivo.

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Section: Discussionmentioning

confidence: 99%

Section: Sub-g1 Apoptosis Assay Using Flow Cytometrymentioning

confidence: 99%

Modified PAMAM vehicles for effective TRAIL gene delivery to colon adenocarcinoma:in vitroandin vivoevaluation

Pishavar

Attaranzadeh

Alibolandi

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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“… Dormancy state: CSCs enter dormancy to evade the attack by the immune system, awaiting new signals from the environment to re-enter the cell cycle [ 22 ]. Changes in morphology or biological function, such as the over-expression of anti-apoptotic proteins that block the cell from entering the type I apoptosis cycle [ 1 , 22 , 24 ]. Elevated expression of ATP-binding cassette (ABC) pumps and detoxifying enzymes to increase the drug’s efflux, which is considered to be an important mechanism for multi-drug resistance (MDR).…”

Section: Cancer Stem Cell Biologymentioning

confidence: 99%

“…Changes in morphology or biological function, such as the over-expression of anti-apoptotic proteins that block the cell from entering the type I apoptosis cycle [ 1 , 22 , 24 ].…”

Section: Cancer Stem Cell Biologymentioning

confidence: 99%

Nanoparticles for Targeted Drug Delivery to Cancer Stem Cells: A Review of Recent Advances

et al. 2021

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Cancer stem cells (CSCs) are a subpopulation of cells that can initiate, self-renew, and sustain tumor growth. CSCs are responsible for tumor metastasis, recurrence, and drug resistance in cancer therapy. CSCs reside within a niche maintained by multiple unique factors in the microenvironment. These factors include hypoxia, excessive levels of angiogenesis, a change of mitochondrial activity from aerobic aspiration to aerobic glycolysis, an upregulated expression of CSC biomarkers and stem cell signaling, and an elevated synthesis of the cytochromes P450 family of enzymes responsible for drug clearance. Antibodies and ligands targeting the unique factors that maintain the niche are utilized for the delivery of anticancer therapeutics to CSCs. In this regard, nanomaterials, specifically nanoparticles (NPs), are extremely useful as carriers for the delivery of anticancer agents to CSCs. This review covers the biology of CSCs and advances in the design and synthesis of NPs as a carrier in targeting cancer drugs to the CSC subpopulation of cancer cells. This review includes the development of synthetic and natural polymeric NPs, lipid NPs, inorganic NPs, self-assembling protein NPs, antibody-drug conjugates, and extracellular nanovesicles for CSC targeting.

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“…This is in part due to their high aqueous solubility and biocompatibility, low immunogenicity, and the presence of modifiable peripheral functional groups [23, 24]. The high density of terminal groups and internal cavities in the PAMAM and PPI dendrimers promotes drug association, resulting in the improvement of aqueous solubility of lipophilic therapeutic compounds [25] such as candersatan cilexetil [26], hydrochlorothiazide [27], crocetin [28] and doxorubicin [29]. Also, several ionic drugs such as mycophenolic acid [30], daidzein [31] phenobarbital and zulfamethoxazole [32] have been associate to dendrimeric carriers.…”

Section: Introductionmentioning

confidence: 99%

Formation of dendrimer-guest complexes as a strategy to increase the solubility of a phenazine N, N′-dioxide derivative with antitumor activity

Dib

Fernández

Santo

et al. 2019

Heliyon

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Poly(amidoamine) and Poly(propylenimine) dendrimers with different generations and peripheral groups were studied as solubility enhancers and nanocarriers for 7-bromo-2-hydroxy-phenazine N 5 , N 10 -dioxide. This compound possesses potential antitumoral and anti-trypanosomal activity, but its low solubility in physiological media precludes its possible application as therapeutic drug. The amino terminated dendrimers association with the active compounds as observed trough NMR studies showed that electrostatic interactions are essential in the solubilization enhancement process. The obtaining of a stable and no cytotoxic formulation makes the drug-carried association a suitable strategy for the generation of a drug delivery system for phenazine derivatives.

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Comparison study of the effect of alkyl-modified and unmodified PAMAM and PPI dendrimers on solubility and antitumor activity of crocetin

Cited by 20 publications

References 35 publications

Modified PAMAM vehicles for effective TRAIL gene delivery to colon adenocarcinoma:in vitroandin vivoevaluation

Modified PAMAM vehicles for effective TRAIL gene delivery to colon adenocarcinoma:in vitroandin vivoevaluation

Nanoparticles for Targeted Drug Delivery to Cancer Stem Cells: A Review of Recent Advances

Formation of dendrimer-guest complexes as a strategy to increase the solubility of a phenazine N, N′-dioxide derivative with antitumor activity

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