Compartmentalized microfluidic perfusion system to culture human induced pluripotent stem cell aggregates

Kondo, Yuki; Hattori, Koji; Tashiro, Shota; Nakatani, Eri; Yoshimitsu, Ryosuke; Satoh, Taku; Sugiura, Shinji; Kanamori, Toshiyuki; Ohnuma, Kiyoshi

doi:10.1016/j.jbiosc.2017.03.014

Cited by 5 publications

(1 citation statement)

References 30 publications

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“…Through this, the analysis of a small number of cancer spheroids from a patient is possible, which could provide a valuable tool for personalized medicine . Alternatively, trapping of multicellular spheroids was used to culture human‐induced pluripotent stem cells and to study the effects of cell differentiation on the spheroid properties . It would be desirable to study the developments, mechanisms, and reactions to external stimuli using these microfluidic trapping devices.…”

Section: Introductionmentioning

confidence: 99%

Micropillar‐based microfluidic device to regulate neurite networks of uniform‐sized neurospheres

et al. 2018

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The inability of neurons to undergo mitosis renders damage to the central or peripheral nervous system. Neural stem cell therapy could provide a path for treating the neurodegenerative diseases. However, reliable and simple tools for the developing and testing neural stem cell therapy are still required. Here, we show the development of a micropillar-based microfluidic device to trap the uniform-sized neurospheres. The neurospheres trapped within micropillar arrays were largely differentiated into neuronal cells, and their neurite networks were observed in the microfluidic device. Compared to conventional cultures on glass slides, the neurite networks generated with this method have a higher reproducibility. Furthermore, we demonstrated the effect of thapsigargin on the neurite networks in the microfluidic device, demonstrating that neural networks exposed to thapsigargin were largely diminished and disconnected from each other. Therefore, this micropillar-based microfluidic device could be a potential tool for screening of neurotoxins.

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Section: Introductionmentioning

confidence: 99%

Micropillar‐based microfluidic device to regulate neurite networks of uniform‐sized neurospheres

et al. 2018

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Towards Three-Dimensional Dynamic Regulation and In Situ Characterization of Single Stem Cell Phenotype Using Microfluidics

Sart

Agathos

2018

Mol Biotechnol

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Mesenchymal stem cells and pluripotent stem cells are recognized as promising tools for tissue engineering, cell therapy, and drug screening. Their use in therapy requires the production of a sufficient number of cells committed to functional regenerative phenotypes. Time- and magnitude-controlled application of mechanical and biochemical cues is required to appropriately control the evolution of stem cell phenotype in 3D. The temporal monitoring of the impact of these cues on the diverse fates of individual stem cells is also needed to ensure the reliability of the differentiation processes. However, macro-scale bioreactors are limited in regulating stem environment and display limited capability to monitor heterogeneities at the single cell level. In turn, microfluidics devices are emerging as powerful tools for tightly controlling culture parameters and precisely monitoring stem cell behavior. This work summarizes recent advances in the applications of microfluidics for the dynamic regulation and characterization of stem cells in 3D.

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Advanced microtechnologies for high-throughput screening

Rodrigues

Fernandes

Diogo

et al. 2020

Engineering Strategies for Regenerative Medicine

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Compartmentalized microfluidic perfusion system to culture human induced pluripotent stem cell aggregates

Cited by 5 publications

References 30 publications

Micropillar‐based microfluidic device to regulate neurite networks of uniform‐sized neurospheres

Micropillar‐based microfluidic device to regulate neurite networks of uniform‐sized neurospheres

Towards Three-Dimensional Dynamic Regulation and In Situ Characterization of Single Stem Cell Phenotype Using Microfluidics

Advanced microtechnologies for high-throughput screening

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