Compatibilidad in vitro de un bioplaguicida a base de Lecanicillium lecanii (Hypocreales: Clavicipitaceae) con agroquímicos empleados en los cultivos de algodón y berenjena

Santos, Adriana Marcela; Grijalba, Érika; Zuluaga, María Victoria; Gómez, Martha Isabel; Villamizar, Laura

doi:10.15446/rev.colomb.biote.v15n2.38025

Cited by 5 publications

(2 citation statements)

References 26 publications

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“…The compatibility of azadirachtin and thiamethoxam, ranked within the tetranortriterpenoide and neonicotinoid chemical groups, respectively, with the isolates of the entomopathogenic fungus B. bassiana, I. sinclairii, and M. rileyi could be related to the low toxicity of these chemical molecules for microorganisms, but also by the possible capacity of entomopathogen to degrade these compounds aiming development and reproduction (Batista Filho et al 2001, Botelho and Monteiro 2011, Hern andez et al 2012, Niassy et al 2012, Santos et al 2013, Silva et al 2013. Different studies have shown the possibility of using these active ingredients with different species of entomopathogenic fungi, showing positive results on the issues focused on compatibility (Batista Filho et al 2001, Andal o et al 2004, Botelho and Monteiro 2011, Hern andez et al 2012, Ribeiro et al 2012, Rocha et al 2012, Cintra et al 2013.…”

Section: Resultsmentioning

confidence: 99%

Potential of Entomopathogenic Fungi as Biological Control Agents of Diamondback Moth (Lepidoptera: Plutellidae) and Compatibility With Chemical Insecticides

Duarte¹,

Gonçalves²,

Espinosa³

et al. 2016

J Econ Entomol

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The objectives were to evaluate the efficiency of entomopathogenic fungi against Plutella xylostella (L.) and the compatibility of the most virulent isolates with some of the insecticides registered for use on cabbage crops. Pathogenicity tests used isolates of Beauveria bassiana, Metarhizium rileyi, Isaria fumosorosea, Isaria sinclairii, and Lecanicillium muscarium standardized at a concentration of 10(7) conidia/ml. Cabbage leaf discs were immersed in these suspensions, and after evaporation of the excess water, were placed 10 second-instar larvae of P. xylostella, totaling 10 leaf discs per treatment. Mortality was assessed 7 d after treatment, and the isolates that caused mortality>80% were used to estimate LC50 and LT50. The compatibilities of the most virulent isolates and the insecticides were tested from the mixture of these into the culture medium, and after solidifying, the medium was inoculated with an aliquot of the isolated suspension. The following parameters were evaluated: growth of the colony, number and viability of conidia after 7 d. The isolated IBCB01, IBCB18, IBCB66, and IBCB87 of B. bassiana, LCMAP101 of M. rileyi, and ARSEF7973 of I. sinclairii caused mortality between 80 and 100%, with LC50 and LT50 between 2.504 to 6.775×10(4) conidia/ml and 52.22 to 112.13 h, respectively. The active ingredients thiamethoxam and azadirachtin were compatible with the entomopathogenic fungi. The results suggest that the use of these isolates is an important alternative in the pesticidal management of P. xylostella, with the possible exception of the associated use of chemical controls using the active ingredients thiamethoxam or azadirachtin.

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Section: Resultsmentioning

confidence: 99%

Potential of Entomopathogenic Fungi as Biological Control Agents of Diamondback Moth (Lepidoptera: Plutellidae) and Compatibility With Chemical Insecticides

Duarte¹,

Gonçalves²,

Espinosa³

et al. 2016

J Econ Entomol

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“…Considering that, this bioproduct is applied with several pesticides (primarily insecticides and fungicides) to reduce the damage caused by different pests and fungal diseases; therefore, compatibility testing is required to ensure the viability, persistence and efficacy of the entomopathogen against the whitefly (Khun et al, 2020; Wari et al, 2020). The previous compatibility tests of Lecabiol WG® with insecticides and fungicides (Santos Díaz et al, 2013) were carried out to evaluate the detrimental effect on fungal germination; however, other variables and conditions should be considered to decrease the variability of compatibility of entomopathogens with pesticides in planta. In addition, new and different pesticides are often launched for emerging diseases and integrated pest management over the years, making it necessary to update compatibility testing.…”

Section: Introductionmentioning

confidence: 99%

In vitro versus in planta: Comparing the compatibility of Akanthomyces lecanii with pesticides against Trialeurodes vaporariorum

Mejía

Espinel

2022

J Applied Entomology

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Lecabiol® is a registered biopesticide based on the entomopathogenic fungus Akanthomyces lecanii (Hypocreales: Cordycipitaceae) for the control of the greenhouse whitefly Trialeurodes vaporariorum (Hemiptera: Aleyrodidae) in tomato crops. This product is commonly applied with several pesticides and fungicides in integrated pest management programs, and a reduction in efficacy is expected. The present work aimed to evaluate the compatibility of A. lecanii with pesticides used in tomato. Three insecticides (bifenthrin, chlorfenapyr and spinetoram) and six fungicides (dimethomorph, kasugamycin, metalaxyl:mancozeb, difenoconazole, pyrimethanil and propineb: cymoxanil) were tested in vitro and in planta. The compatibility was determined by using the biological index (BI). For in vitro test, germination, mycelial growth and conidial production were evaluated, and for in planta, the mixture of pesticide + fungus and alternating applications was carried out to assess the efficacy to control the pest. The insecticide chlorfenapyr was compatible in vitro (BI = 78) but moderately toxic (BI = 52) when the product was applied as a mixture with the fungus to control T. vaporariorum in planta; in contrast, bifenthrin was initially classified as moderately toxic (BI = 57) but was compatible in planta (BI = 71). The fungicides dimethomorph and kasugamycin were compatible (BI = 81–97); propineb: cymoxanil was moderately toxic (BI = 51), and metalaxyl:mancozeb and difenoconazole were toxic (BI = 0–37) in vitro, whereas most fungicides were moderately toxic or toxic when evaluated in planta. The pesticides spinetoram and pyrimethanil remained compatible in both assays (BI = 85–90). For the alternating application, the fungicides dimethomorph and pyrimethanil significantly diminished the efficacy of the fungi below 40% to control T. vaporariorum. The findings demonstrate that compatibility of the fungus with insecticides and fungicides varied among in vitro and in planta assessments (combined or via alternation).

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In vitro compatibility with soybean agrochemicals and storage stability studies of the Beauveria bassiana biopesticide

Garcia-Riaño

Torres-Torres

Santos-Díaz

et al. 2022

Biocatalysis and Agricultural Biotechnology

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Compatibilidad in vitro de un bioplaguicida a base de Lecanicillium lecanii (Hypocreales: Clavicipitaceae) con agroquímicos empleados en los cultivos de algodón y berenjena

Cited by 5 publications

References 26 publications

Potential of Entomopathogenic Fungi as Biological Control Agents of Diamondback Moth (Lepidoptera: Plutellidae) and Compatibility With Chemical Insecticides

Potential of Entomopathogenic Fungi as Biological Control Agents of Diamondback Moth (Lepidoptera: Plutellidae) and Compatibility With Chemical Insecticides

In vitro versus in planta: Comparing the compatibility of Akanthomyces lecanii with pesticides against Trialeurodes vaporariorum

In vitro compatibility with soybean agrochemicals and storage stability studies of the Beauveria bassiana biopesticide

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