Compatibility and combination of selected bacterial antagonists in the biocontrol of sisal bole rot disease

Barbosa, Leonardo O.; Lima, Jéssica Souza; Magalhães, Valter Cruz; Gava, C. A. T.; Soares, Ana Cristina Fermino; Marbach, Phellippe Arthur Santos; Souza, Jorge Teodoro de

doi:10.1007/s10526-018-9872-x

Cited by 14 publications

(22 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have indicated a range of biocontrol mechanisms may be operative in mixed populations of microorganisms, which may provide enhanced [47][48][49], reduced [50][51][52], or similar biocontrol efficacy [53][54][55]. Importantly, however, the relative significance of single and combinations of organisms in biocontrol activity was not established since testing for synergistic, enhanced, or antagonistic interactions among the microorganisms in controlling diseases was not conducted or statistically evaluated.…”

Section: Discussionmentioning

confidence: 99%

Yeasts and Bacterial Consortia from Kefir Grains Are Effective Biocontrol Agents of Postharvest Diseases of Fruits

et al. 2020

View full text Add to dashboard Cite

Fungal pathogens in fruits and vegetables cause significant losses during handling, transportation, and storage. Biological control with microbial antagonists replacing the use of chemical fungicides is a major approach in postharvest disease control, and several products based on single antagonists have been developed but have limitations related to reduced and inconsistent performance under commercial conditions. One possible approach to enhance the biocontrol efficacy is to broaden the spectrum of the antagonistic action by employing compatible microbial consortia. Here, we explore commercial kefir grains, a natural probiotic microbial consortium, by culture-dependent and metagenomic approaches and observed a rich diversity of co-existing yeasts and bacterial population. We report effective inhibition of the postharvest pathogen Penicillium expansum on apple by using the grains in its fresh commercial and milk-activated forms. We observed few candidate bacteria and yeasts from the kefir grains that grew together over successive enrichment cycles, and these mixed fermentation cultures showed enhanced biocontrol activities as compared to the fresh commercial or milk-activated grains. We also report several individual species of bacteria and yeasts with biocontrol activities against Penicillium rots on apple and grapefruit. These species with antagonistic properties could be further exploited to develop a synthetic consortium to achieve enhanced antagonistic effects against a wide range of postharvest pathogens.

show abstract

Section: Discussionmentioning

confidence: 99%

Yeasts and Bacterial Consortia from Kefir Grains Are Effective Biocontrol Agents of Postharvest Diseases of Fruits

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Additionally, these strains generally inhibited phytopathogenic fungi, while little or no significant effects were observed against fungi that are antagonistic to M. incognita . These two strains were previously tested for their compatibility in vitro and were shown to be incompatible and for this reason were not tested in experiments in planta 38 . Therefore, in this study, we further investigated whether this incompatibility affects their beneficial activities.…”

Section: Discussionmentioning

confidence: 99%

The combination of two Bacillus strains suppresses Meloidogyne incognita and fungal pathogens, but does not enhance plant growth

Cruz-Magalhães

Guimarães

Silva

et al. 2021

Pest Management Science

Self Cite

View full text Add to dashboard Cite

BACKGROUND The combination of biocontrol agents is a desirable strategy to improve control efficacy against the root‐knot nematode (RKN) Meloidogyne incognita under field conditions. However, strains compatibility is generally tested in vitro and incompatible combinations are normally not further examined in experiments in planta. Therefore, there is virtually no information on the performance of incompatible strains. In this study, we evaluated two Bacillus strains previously described as incompatible in vitro for effects on plant growth and suppression of M. incognita, pathogenic fungi and nematophagous fungi. RESULTS Strains BMH and INV were shown to be closely related to Bacillus velezensis. These strains, when applied individually, reduced the number of galls and eggs of M. incognita by more than 90% in tomato roots. When BMH and INV were combined (BMH + INV), RKN suppression and tomato shoot weight were lower compared to single‐strain applications. Additionally, metabolites in cell‐free supernatants and volatile organic compounds (VOCs) from strains BMH and INV had strong effects against the plant pathogens M. incognita, Fusarium oxysporum, Rhizoctonia solani and Sclerotium rolfsiii, but not against three species of nematophagous fungi. Although strain INV and the combination BMH + INV emitted fewer VOCs than strain BMH, they were still capable of killing second‐stage juveniles of M. incognita. CONCLUSIONS Bacillus strains BMH and INV inhibited M. incognita and fungal pathogens, and promoted tomato growth. However, strain INV emitted fewer VOCs and the combination BMH + INV did not enhance the activity of the biocontrol strains against the RKN or their capacity to promote plant growth. © 2021 Society of Chemical Industry.

show abstract

“…Another method investigated to manage the disease is the use of antagonistic microorganisms [53,34]. Chemical control was never investigated probably because the causal agents are soilborne fungi and farmers have little financial resources.…”

Section: Disease Managementmentioning

confidence: 99%

“…Biological control is an environmentally friendly and viable method to control plant pathogens [54,45]. Antagonistic bacteria were shown to have potential to control the bole rot disease [53,34]. Several strains of an undescribed species of Burkholderia and strains of Bacillus decreased the incidence and severity of the disease under field conditions (Figures 5 and 6) [53,34].…”

Section: Disease Managementmentioning

confidence: 99%

“…Antagonistic bacteria were shown to have potential to control the bole rot disease [53,34]. Several strains of an undescribed species of Burkholderia and strains of Bacillus decreased the incidence and severity of the disease under field conditions (Figures 5 and 6) [53,34]. Therefore, it is possible to establish programmes aimed at the development of biological products to manage the disease in the field.…”

Section: Disease Managementmentioning

confidence: 99%

See 1 more Smart Citation

Sisal Bole Rot: An Important but Neglected Disease

Cruz-Magalhães¹,

Andrade²,

Figueiredo³

et al. 2020

Plant Diseases - Current Threats and Management Trends

View full text Add to dashboard Cite

Sisal (Agave sisalana) is one of the main sources of hard natural fibre and raw materials for the industry, medicine and handicrafts. Sisal yields a coarse and strong fibre that is increasingly being used in composite materials for automobiles, furniture, construction and plastic and paper products. Extracts of sisal contain substances with anti-inflammatory, antimicrobial and anthelmintic activities. Sisal is adapted to warm environments with low rainfall and is an excellent option for cultivation in semiarid conditions, where other crops cannot be grown. The world's largest sisal producers are Brazil, Tanzania, China, Kenya and Madagascar. Sisal is a labour-intensive crop with great socio-economical importance as it is cultivated in poor areas employing familiar labour. Sisal bole rot is the main disease of sisal, responsible for substantial losses in producing countries. The disease is caused by certain species of the genus Aspergillus, especially the ones belonging in the section Nigri. The main symptoms are yellowing of the aerial parts and the red-coloured rot of the bole, which causes the plant to die. In this review we are going to address the taxonomy of the causal agents, disease diagnosis and epidemiology and disease management, with emphasis on biological control.

show abstract

Compatibility and combination of selected bacterial antagonists in the biocontrol of sisal bole rot disease

Cited by 14 publications

References 30 publications

Yeasts and Bacterial Consortia from Kefir Grains Are Effective Biocontrol Agents of Postharvest Diseases of Fruits

Yeasts and Bacterial Consortia from Kefir Grains Are Effective Biocontrol Agents of Postharvest Diseases of Fruits

The combination of two Bacillus strains suppresses Meloidogyne incognita and fungal pathogens, but does not enhance plant growth

Sisal Bole Rot: An Important but Neglected Disease

Contact Info

Product

Resources

About