Compensatory cell proliferation in the kidney after unilateral nephrectomy in mice

Skraastad, Øyvind

doi:10.1007/bf02890230

Cited by 13 publications

(9 citation statements)

References 15 publications

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“…A bimodal pattern of mitotic rate and DNA-specific activity has been reported after Ux [2], Peak activity was demonstrated at 40-48 h followed by a smaller sustained wave of activity at 60-120 h. This biphasic nature of DNA synthesis and replication was also demonstrated in the present study. In cortical cells, peak synthetic activity was demonstrated at 12 and 120 h, and peak replication at 24 and 120 h in both groups.…”

Section: Whole Blood Csa Levelsupporting

confidence: 63%

“…Many laboratory methods for detecting cellular prolif eration have been employed including morphometric analysis [10], measurements of polyamine metabolism [11], and tritiated thymidine labelling studies [2], but these methods are often difficult and time-consuming. In this study, we used FCMX to detect cellular proliferation since FCMX provides a rapid, objective DNA analysis of Am J Nephrol 1997:17:483-486individual cells, and FCMX-generated DNA histograms graphically represent the various phases of the cell cycle [ 12].…”

Section: Whole Blood Csa Levelmentioning

confidence: 99%

“…Unilateral renal loss results in a compensatory renal growth (CRG) response which consists of cellular hyper trophy [1] and cellular proliferation [2]. This change is comparable to the transplanted kidney, and an adaptive increase in the glomerular filtration rate in kidneys after transplantation is related to CRG.…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of Cellular Proliferation by Cyclosporin A during Compensatory Renal Growth: Flow Cytometric Analysis

Yang¹,

Kim

et al. 1997

Am J Nephrol

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Cell cycle analysis using flow cytometer (FCMX) was performed to evaluate the influence of cyclosporin A (CsA) on cellular proliferation during compensatory renal growth (CRG) in uninephrectomized (Ux) rats. CsA (50 mg/kg/ day) was administered subcutaneously for 7 days and animals were scarificed 0, 12,24, 48, 72, 120 and 168 h after Ux. The percent changes in the S (DNA synthesis) and G₂M (DNA replication) phases in the contralateral kidney were analyzed. The cortical cells entering the S and G₂M phases showed significant differences between CsA and vehicle groups (p = 0.0001 p = 0.001 respectively), but the medullary cells entering the S and G2M phases showed no significant differences between the 2 groups (p = 1.000, respectively). In the cortex, the CsA group showed a significant decrease in the S (at 12, 72 and 120 h) and G₂M phases (at 24, 72 and 120 h) compared to the vehicle group (p < 0.05, respectively), but there was no significant difference in the medulla. In conclusion, cellular proliferation is suppressed in cortical cells by CsA.

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Section: Whole Blood Csa Levelsupporting

confidence: 63%

Section: Whole Blood Csa Levelmentioning

confidence: 99%

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Inhibition of Cellular Proliferation by Cyclosporin A during Compensatory Renal Growth: Flow Cytometric Analysis

Yang¹,

Kim

et al. 1997

Am J Nephrol

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“…Several authors (4,5] contend that this increase in DNA is secon dary to cellular replication (hyperplasia). A variety of meth odologies have been employed to test this hypothesis in-eluding morphometric analysis [6], measurements of poly amine metabolism [7], and tritiated thymidine labeling stud ies [3]. Although the results from previous studies substan tiate the finding of increased DNA synthesis, they are unable to differentiate between actual cellular replication and nuclear polyploidy.…”

Section: Introductionmentioning

confidence: 63%

“…my [1], This event appears to occur in the cortically located convoluted tubules [2]. In addition to cellular hypertrophy, a small but consistent increase in the total DNA content of the remaining cells has also been observed in the contrala teral kidney following unilateral nephrectomy [3]. Several authors (4,5] contend that this increase in DNA is secon dary to cellular replication (hyperplasia).…”

Section: Introductionmentioning

confidence: 99%

Detection of Cellular Proliferation during Compensatory Renal Growth using Flow Cytometry

Rin¹,

Benson²,

Bandyk³

et al. 1992

Nephron

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Compensatory renal growth (CRG) consists of cellular enlargement and a small but consistent increase in DNA content. It has been assumed that the increase in total renal DNA content was due to new cell formation, however, the possibility of nuclear polyploidy remained an alternative explanation. To test the hypothesis whether cellular hyperplasia is the cause of the increase in DNA content during compensatory growth after renal deprivation, we performed cell cycle analysis using flow cytometry. Following unilateral nephrectomy, the amount of cortical cells in the S phase increased by 12% at 10 h while the number of cells in the G₂M phase increased by 7% at 120 h. Medullary cells entering the S phase increased by 26% at 24 h and those in G₂M increased by 12% at 168 h. DNA synthesis and replication occurs during CRG following unilateral nephrectomy as evidenced by an increase in cells entering both the S and G₂M phases of the cell cycle. The increase in DNA content during CRG is a result of cellular proliferation and not polyploidy.

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Expression of Differentiated Function in Neoplasms

McKinnell

1989

The Pathobiology of Neoplasia

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Compensatory cell proliferation in the kidney after unilateral nephrectomy in mice

Cited by 13 publications

References 15 publications

Inhibition of Cellular Proliferation by Cyclosporin A during Compensatory Renal Growth: Flow Cytometric Analysis

Inhibition of Cellular Proliferation by Cyclosporin A during Compensatory Renal Growth: Flow Cytometric Analysis

Detection of Cellular Proliferation during Compensatory Renal Growth using Flow Cytometry

Expression of Differentiated Function in Neoplasms

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