Competing beetles attract egg laying in a hawkmoth

Zhang, Jin; Raza, Syed Ali; Wei, Zhiqiang; Keesey, Ian W.; Parker, Anna L.; Feistel, Felix; Chen, Jingyuan; Cassau, Sina; Fandino, Richard A.; Große-Wilde, Ewald; Dong, Shuang-Lin; Kingsolver, Joel G.; Gershenzon, Jonathan; Knaden, Markus; Hansson, Bill S.

doi:10.1016/j.cub.2021.12.021

Cited by 22 publications

(25 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We used the nCounter XT CodeSet gene expression assay (NanoString Technologies, Inc., United States 4 ). The custom CodeSet (Zhang et al, 2022) contained 268 probes targeting 71 ORs, 29 IRs, 49 GRs, 47 odorant binding proteins, 5 pickpocket, 3 sensory neuron membrane proteins and 62 candidate reference gene transcripts (Supplementary Table 2). The probes were designed using the reference coding sequences of 74 ORs, 21 IRs, and 45 GRs (Koenig et al, 2015) that were submitted to M. sexta OGS2.0 (Kanost et al, 2016).…”

Section: Nanostring Gene Expression Assaymentioning

confidence: 99%

“…This paved the way for the application of CRISPR-Cas9 mediated targeted knock-out experiments revealing the function of the obligate co-receptors MsexORCo for ORs, and MsexIR8a and MsexIR25a for IRs in M. sexta (Fandino et al, 2019;. In addition, one sex pheromone receptor and two ORs detecting plant volatiles have been deorphanized to date (Wicher et al, 2017;Guo et al, 2021;Zhang et al, 2022), whereas the functions of all other chemosensory receptors of M. sexta remain elusive to the best of our knowledge. A critical step in understanding any gene's function is knowing when and where it is expressed.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sex- and tissue-specific expression of chemosensory receptor genes in a hawkmoth

Tom

Llorca²,

Bucks³

et al. 2022

Front. Ecol. Evol.

Self Cite

View full text Add to dashboard Cite

For the nocturnal hawkmoth Manduca sexta, olfactory and gustatory cues are essential for finding partners, food, and oviposition sites. Three chemosensory receptor families, odorant receptors (ORs), ionotropic receptors (IRs), and gustatory receptors (GRs) are involved in the detection of these stimuli. While many chemosensory receptor genes have been identified, knowledge of their expression profile in potentially chemoreceptive organs is incomplete. Here, we studied the expression patterns of chemosensory receptors in different tissues including the antennae, labial palps, proboscis, legs, wings and ovipositor. We compared the receptors’ expression in female and male moths both before and after mating by using the NanoString platform. This tool allowed us to measure expression levels of chemosensory receptor genes in a single reaction using probes designed against 71 OR, 29 IR and 49 GR transcripts. In all tissues investigated, we detected expression of genes from all three receptor families. The highest number of receptors was detected in the antennae (92), followed by the ovipositor (59), while the least number was detected in the hindlegs (21). The highest number of OR genes were expressed in the antennae (63), of which 24 were specific to this main olfactory organ. The highest number of IRs were also expressed in the antennae (16), followed by the ovipositor (15). Likewise, antennae and ovipositor expressed the highest number of GRs (13 and 14). Expression of the OR co-receptor MsexORCo, presumably a prerequisite for OR function, was found in the antennae, labial palps, forelegs and ovipositor. IR co-receptors MsexIR25a and MsexIR76b were expressed across all tested tissues, while expression of the IR co-receptor MsexIR8a was restricted to antennae and ovipositor. Comparing the levels of all 149 transcripts across the nine tested tissues allowed us to identify sex-biased gene expression in the antennae and the legs, two appendages that are also morphologically different between the sexes. However, none of the chemosensory receptors was differentially expressed based on the moths’ mating state. The observed gene expression patterns form a strong base for the functional characterization of chemosensory receptors and the understanding of olfaction and gustation at the molecular level in M. sexta.

show abstract

Section: Nanostring Gene Expression Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sex- and tissue-specific expression of chemosensory receptor genes in a hawkmoth

Tom

Llorca²,

Bucks³

et al. 2022

Front. Ecol. Evol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The olfactory behaviors of insects are important clues for targeting foods, mating and avoiding natural enemies [ 28 , 29 ]. The oviposition behavior was disturbed after the Or35 deficiency in the tobacco hawkmoths Manduca sexta [ 31 ] and the Or31 deficiency in Helicoverpa assulta [ 52 ]. In this study, we noted that the feeding preferences have been disturbed, from preference to non-preference for SRBSDV rice plants, after M. anisopliae infection.…”

Section: Discussionmentioning

confidence: 99%

“…They could identify special chemical odor molecules and locate the odor source by olfactory sensation and exhibit a behavioral response correspondingly [ 28 , 30 ]. For instance, the tobacco hawkmoths Manduca sexta preferentially oviposit on the jimson weed Datura wrightii due to the α-copaene induced via potato beetle Lema daturaphila infection [ 31 ]; the 4-vinylanisole is an important pheromone that could cause an aggregation behavior of the migratory locust Locusta migratoria [ 32 ]. The rice planthopper S. furcifera also shows a feeding preference for rice plants infected by the SRBSDV virus [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

The Entomopathogenic Fungus Metarhizium anisopliae Affects Feeding Preference of Sogatella furcifera and Its Potential Targets’ Identification

Wang

Han

Xia

et al. 2022

JoF

View full text Add to dashboard Cite

The rice planthopper Sogatella furcifera is a unique vector of the southern rice black-streaked dwarf virus (SRBSDV). The feeding behavior of S. furcifera should directly affect the diffusion of this virus. In this study, we noted that the infection of Metarhizium anisopliae CQMa421 on S. furcifera disturbed the feeding behavior of this pest to SRBSDV-infected rice, from preference to non-preference. Then, we further investigated the potential targets of M. anisopliae CQMa421 on the feeding behavior of S. furcifera after 0 h, 24 h and 48 h of infection by transcriptomic analysis via Illumina deep sequencing. A total of 93.27 GB of data was collected after sequencing, from which 91,125 unigenes were annotated, including 75 newly annotated genes. There were 1380 vs. 2187 and 137 vs. 106 upregulated and downregulated differentially expressed genes (DEGs) detected at 24 h and 48 h, respectively. The biological functions and associated metabolic processes of these genes were determined with the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. The results suggested that major of DEGs are involved in energy metabolism, biosynthesis, immune response, the FoxO signaling pathway, the MAPK signaling pathway and apoptosis in response to the fungal infection. Noteworthily, several olfactory-related genes, including odorant receptors and odorant binding proteins, were screened from these differentially expressed genes, which played critical roles in regulating the olfactory behavior of insects. Taken together, these results provide new insights for understanding the molecular mechanisms underlying fungus and host insect interaction, especially for olfactory behavior regulated by fungus.

show abstract

“…The high sensitivity towards these odors might indicate that they act as long-distance cues guiding the moth to places with vegetation (Webster & Carde, 2017). Furthermore, these odors have meanings that are more specific: alpha-copaene is involved in the oviposition decision process of M. sexta (Zhang et al, 2022) and in addition might indicate rewarding nectar sources as it was functionally present, i.e. EAD-active, only in the headspace of Datura flower and Mirabilis .…”

Section: Discussionmentioning

confidence: 99%

Unique neural coding of crucial versus irrelevant plant odors in a hawkmoth

Bisch-Knaden

Knaden

Hansson

2022

Preprint

Self Cite

View full text Add to dashboard Cite

The sense of smell is pivotal for nocturnal moths to locate feeding and oviposition sites. However, these crucial resources are often rare and their bouquets are intermingled with volatiles emanating from surrounding ‘background’ plants. Here we asked if the olfactory system of female hawkmoths, Manduca sexta, could differentiate between crucial and background cues. To answer this question, we collected nocturnal headspaces of numerous plants in a natural habitat of M. sexta. We analyzed the chemical composition of these headspaces, and used them as stimuli in physiological experiments at the antenna and in the brain. The intense odors of floral nectar sources evoked strong responses in virgin and mated female moths, most likely enabling the localization of profitable flowers at a distance. Bouquets of larval host plants and most background plants, in contrast, were subtle, thus potentially complicating host identification. However, despite being subtle, antennal responses and brain activation patterns evoked by the smell of larval host plants were clearly different from those evoked by other plants. Interestingly, this difference was even more pronounced in the antennal lobe of mated females, revealing a status-dependent tuning of their olfactory system towards oviposition sites. Our study suggests that female moths possess unique neural coding strategies to find not only conspicuous floral cues but also inconspicuous bouquets of larval host plants within a complex olfactory landscape.

show abstract

Competing beetles attract egg laying in a hawkmoth

Cited by 22 publications

References 49 publications

Sex- and tissue-specific expression of chemosensory receptor genes in a hawkmoth

Sex- and tissue-specific expression of chemosensory receptor genes in a hawkmoth

The Entomopathogenic Fungus Metarhizium anisopliae Affects Feeding Preference of Sogatella furcifera and Its Potential Targets’ Identification

Unique neural coding of crucial versus irrelevant plant odors in a hawkmoth

Contact Info

Product

Resources

About