Competition for glucose between the yeasts Saccharomyces cerevisiae and Candida utilis

Postma, Erik; Kuiper, Arthur; Tomasouw, W F; Scheffers, W. A.; Dijken, Johannes P. van

doi:10.1128/aem.55.12.3214-3220.1989

Cited by 56 publications

(30 citation statements)

References 30 publications

(38 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Culture dry weights were determined by filtering, washing, and drying culture samples, essentially as described in [14]. However, here filters were dried overnight in a 60°C incubator.…”

Section: Steady-state Measurementsmentioning

confidence: 99%

A new regulatory principle for in vivo biochemistry: Pleiotropic low affinity regulation by the adenine nucleotides – Illustrated for the glycolytic enzymes of Saccharomyces cerevisiae

et al. 2013

View full text Add to dashboard Cite

a b s t r a c tEnzymology tends to focus on highly specific effects of substrates, allosteric modifiers, and products occurring at low concentrations, because these are most informative about the enzyme's catalytic mechanism. We hypothesized that at relatively high in vivo concentrations, important molecular monitors of the state of living cells, such as ATP, affect multiple enzymes of the former and that these interactions have gone unnoticed in enzymology.We test this hypothesis in terms of the effect that ATP, ADP, and AMP might have on the major free-energy delivering pathway of the yeast Saccharomyces cerevisiae. Assaying cell-free extracts, we collected a comprehensive set of quantitative kinetic data concerning the enzymes of the glycolytic and the ethanol fermentation pathways. We determined systematically the extent to which the enzyme activities depend on the concentrations of the adenine nucleotides. We found that the effects of the adenine nucleotides on enzymes catalysing reactions in which they are not directly involved as substrate or product, are substantial. This includes effects on the Michaelis-Menten constants, adding new perspective on these, 100 years after their introduction.

show abstract

“…Culture dry weights were determined by filtering, washing, and drying culture samples, essentially as described in [14]. However, here filters were dried overnight in a 60°C incubator.…”

Section: Steady-state Measurementsmentioning

confidence: 99%

A new regulatory principle for in vivo biochemistry: Pleiotropic low affinity regulation by the adenine nucleotides – Illustrated for the glycolytic enzymes of Saccharomyces cerevisiae

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Cells were grown, harvested in mid-exponential growth phase by centrifugation in a model 4K10 centrifuge (B. Braun, Melsungen, Germany), washed twice and resuspended in ice-cold distilled water containing the same amount of sodium chloride as the growth medium. The ¢nal cell suspension had a concentration of 25^30 (mg dry wt) ml 31 . Respiration was monitored using an O 2 -selective electrode as described in Neves et al [11].…”

Section: Measurement Of Respiratory and Fermentative £Uxesmentioning

confidence: 99%

“…Respiration was monitored using an O 2 -selective electrode as described in Neves et al [11]. The speci¢c respiration rate was expressed as Wmol O 2 consumed min 31 (g dry wt) 31 . CO 2 production rates were determined using a CO 2 -selective electrode (Radiometer, Copenhagen, Denmark E5036-0).…”

Section: Measurement Of Respiratory and Fermentative £Uxesmentioning

confidence: 99%

“…The electrode was coupled to a standard pH/mV meter (Radiometer, PHM 82), connected to a £at recorder (Kipp and Zonen, Delft, The Netherlands) and CO 2 liberation was monitored in a chamber with temperature control and gentle magnetic stirring. The reaction mixture contained 4.5 ml of growth medium without carbon source and 0.25 ml of cellular suspension (25 mg ml 31 ). As soon as the mV value had stabilized, a starter of 0.25 ml of 2 M glucose was added.…”

Section: Measurement Of Respiratory and Fermentative £Uxesmentioning

confidence: 99%

“…The samples were analyzed by atomic absorption spectroscopy (AAS) in a Varian Spectra AA.250 PLUS spectrometer in absorption mode. To avoid potassium and sodium ionization, 1 g l 31 CsCl was added to the samples for the determination of [K þ ] and 2 g l 31 KCl for the determination of [Na þ ]. Sample duplicates were used to con¢rm some of the results using an ion-coupled plasma (ICP) spectrometer, PU 7000 (Unicam), as in Neves et al [11], as well as by 39 K and 23 Na nuclear magnetic resonance (NMR) (see Acknowledgements).…”

Section: Estimation Of Intracellular Concentrations Of Na + and K +mentioning

confidence: 99%

See 2 more Smart Citations

Outlines for the definition of halotolerance/halophily in yeasts: () CBS4019 as the archetype?

Silva-Graça

Neves

Lucas

2003

FEMS Yeast Research

View full text Add to dashboard Cite

Candida versatilis (halophila) CBS4019 was chosen to study the physiological reactions of long-term exposure to extremely high salt concentrations. In general, our results show a significant increase in enzyme expression during growth under stress conditions. Although glycerol and mannitol pathways are not under glucose repression, they were found to be metabolically regulated. Glycerol-3P-dehydrogenase used either of its cofactors NADPH or NADH, being in favor of NADPH during growth with high salt concentrations. This ability of interchanging cofactors, an increased fermentation rate, and the observed mannitol pathway activity are suggested to contribute to the yeasts' redox stability. Enzymes per se were not salt-tolerant in vitro. Consistently, intracellular sodium was low and intracellular potassium, a requirement for growth, was high. The concept of halophily and its applicability to yeasts is discussed.

show abstract

Fed‐Batch Droplet Nanobioreactor for Controlled Growth of Cyberlindnera (Pichia) jadinii: A Proof‐Of‐Concept Demonstration

Totlani

Wang

Bisschops

et al. 2021

Adv Materials Technologies

View full text Add to dashboard Cite

Improvement of the microorganism's product yield on feedstock is possible by targeted genetic modification, which requires a solid understanding of the species metabolism and genetics. Alternatively, genetic modifications can be introduced randomly such that the chance that a resulting mutant performs better than the original one is small. Consequently, many mutants need to be created and subsequently screened in order to identify the best performing ones. [1] Given the large number of experiments, it is common practice to grow and study all the different mutants in individual microliter-sized wells on microtiter plates. While this allows for parallel screening in an automated fashion using pipetting robots and plate readers, conventional microtiter plates lack the ability to feed nutrients and control pH by base or acid addition. It is indeed challenging to feed liquids at flow rates in the nanoliter per hour range to all the individual wells of a microtiter plate and to do so accurately. Therefore, screening of mutants is commonly performed under batch conditions with all feedstock present from the start and no further control over feedstock concentration and pH. In contrast, more than 80% of the processes in industrial biotechnology are operated under so called fed-batch conditions with control over feedstock concentration and pH. [2,3] This incompatibility between the physiological conditions during screening and industrial operation not only leads to selection of false positives which fail to generate competitive yields at industrial scale, but also fails to identify the best mutants for fed-batch conditions. [4][5][6] The effectiveness of screening hence greatly benefits from a technology that enables growing and studying a large number of microorganisms under precisely controlled conditions representative of industrial bioreactors. Besides screening of mutants, optimization of process conditions, a second important aspect in bioprocess development, also benefits from such a technology. [7] Although there has been progress in recent years, there remains both need and opportunity to cost-effectively and with fidelity miniaturize fermentation to screen under fed-batch conditions.Several strategies have been developed to study microorganisms under controlled growth conditions. One strategy is to A key bottleneck in bioprocess development is that state-of-the-art tools used for screening of cells and optimization of cultivation conditions do not represent the conditions enforced at industrial scale. At industrial scale, cell growth is strictly controlled ("fed-batch") to optimize the metabolites produced by the cells. In contrast, cell growth is uncontrolled ("batch") in microwells commonly used for bioprocess development due to the difficulty to continuously supply minute amounts of nutrients to the cells in these wells over the course of the cultivation experiment. This work addresses this bottleneck through the development of a droplet-based fed-batch nanobioreactor.A key challenge addressed in this work is ...

show abstract

Competition for glucose between the yeasts Saccharomyces cerevisiae and Candida utilis

Cited by 56 publications

References 30 publications

A new regulatory principle for in vivo biochemistry: Pleiotropic low affinity regulation by the adenine nucleotides – Illustrated for the glycolytic enzymes of Saccharomyces cerevisiae

A new regulatory principle for in vivo biochemistry: Pleiotropic low affinity regulation by the adenine nucleotides – Illustrated for the glycolytic enzymes of Saccharomyces cerevisiae

Outlines for the definition of halotolerance/halophily in yeasts: () CBS4019 as the archetype?

Fed‐Batch Droplet Nanobioreactor for Controlled Growth of Cyberlindnera (Pichia) jadinii: A Proof‐Of‐Concept Demonstration

Contact Info

Product

Resources

About