2003
DOI: 10.1128/cdli.10.5.835-848.2003
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Competitive Binding Inhibition Enzyme-Linked Immunosorbent Assay That Uses the Secreted Aspartyl Proteinase ofCandida albicansas an Antigenic Marker for Diagnosis of Disseminated Candidiasis

Abstract: The secreted aspartyl proteinases (Saps) of Candida albicans have been implicated as virulence factors associated with adherence and tissue invasion. The potential use of proteinases as markers of invasive candidiasis led us to develop a competitive binding inhibition enzyme-linked immunosorbent assay (ELISA) to detect Sap in clinical specimens. Daily serum and urine specimens were collected from rabbits that had been immunosuppressed with cyclophosphamide and cortisone acetate and infected intravenously with … Show more

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Cited by 21 publications
(21 citation statements)
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“…Rabbit models of disseminated candidiasis and IA, previously described by our laboratory (26,49), were used as the source of infected tissues. DNA extracted from frozen, homogenized kidney tissue was PCR amplified using primers ITS3 and ITS4.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Rabbit models of disseminated candidiasis and IA, previously described by our laboratory (26,49), were used as the source of infected tissues. DNA extracted from frozen, homogenized kidney tissue was PCR amplified using primers ITS3 and ITS4.…”
Section: Resultsmentioning
confidence: 99%
“…Rabbit models of IA and candidiasis developed previously (26,49) were used to detect fungal DNA in tissues from infected animals. Rabbits were euthanized and necropsied on day 3 after infection, and tissue samples (5 by 5 by 1 mm) were placed immediately after removal onto aluminum foil, wrapped tightly, and submerged into liquid nitrogen for 1 min.…”
Section: Vol 42 2004 Rapid Differentiation Of Aspergillus Species Bmentioning
confidence: 99%
“…For example, high titers of anti-Sap antibodies were observed in the sera of patients with candidiasis [123], and Sap antigens were detected in biopsies of oral epithelial lesions collected from HIVinfected patients [105]. Regarding the Saps immunogenic properties, a competitive binding inhibition enzyme-linked immunosorbent assay (ELISA) was described as sensitive and specific for the detection of Sap antigens in urine specimens from rabbits with disseminated C. albicans infection, being a potential antigenic marker to be used in a noninvasive means in order to diagnose disseminated candidiasis [124].…”
Section: Saps: Virulence Attributes With Multiple Biological Functionsmentioning
confidence: 99%
“…Of all Candida species, C. albicans remains the most common species of bloodstream infections in hospitals. C. albicans is responsible for 59% of nosocomial candidemia and for 55% of bloodstream infections [9,10,11]. …”
Section: Introductionmentioning
confidence: 99%