Competitive HRP-Linked Colorimetric Aptasensor for the Detection of Fumonisin B1 in Food based on Dual Biotin-Streptavidin Interaction

Tao, Zui; Zhou, You; Li, Xiang; Wang, Zhouping

doi:10.3390/bios10040031

Cited by 24 publications

(7 citation statements)

References 41 publications

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“…These enzymes play an important role in various metabolic processes in human [15]. Moreover, HRP is often employed in biosensor analysis as an enzymatic tag [16,17]. These are the reasons why this protein has been chosen as a model object for our study.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Influence of Liquid Motion in a Flow-based System on an Enzyme Aggregation State with an Atomic Force Microscopy Sensor: The Effect of Water Flow

Ziborov¹,

Pleshakova²,

Shumov³

et al. 2020

Applied Sciences

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The influence of liquid motion in flow-based systems on the aggregation state of an enzyme and on its enzymatic activity was studied, with horseradish peroxidase (HRP) as an example. Our experiments were carried out in a setup modeling the flow section of the biosensor communication with a measuring cell containing a protein solution. Studies were conducted for a biosensor measuring cell located along the axis of a spiral-moving liquid flow. The aggregation state of the protein was determined with an atomic force microscopy-based sensor (AFM sensor). It has been demonstrated that upon flowing of water through silicone biosensor communications, an increased aggregation of HRP protein was observed, but, at the same time, its enzymatic activity did not change. Our results obtained herein are useful in the development of models describing the influence of liquid flow in biosensor communications on the properties of enzymes and other proteins. This is particularly important for the development of serologic protein biosensors, which are beginning to be used for the early diagnosis of oncological diseases (such as brain cancer, prostate cancer, breast cancer etc.). The results obtained herein should also be taken into account when considering possible changes in hemodynamics due to increased protein aggregation.

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Section: Introductionmentioning

confidence: 99%

Investigation of the Influence of Liquid Motion in a Flow-based System on an Enzyme Aggregation State with an Atomic Force Microscopy Sensor: The Effect of Water Flow

Ziborov¹,

Pleshakova²,

Shumov³

et al. 2020

Applied Sciences

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“…6a, b). The aptasensor exhibits good reproducibility and Fluorescence resonance energy transfer aptasensor 0.1 ng/mL 0.1-500 ng/mL [24] Aptamer-Photonic crystal encoded suspension array 0.16 pg/mL 0.001-1 ng/mL [17] Aptamer-based microcantilever array biosensor 0.33 ng/mL 0.1-40 µg/mL [25] Aptamer based colorimetric assay 0.3 ng/mL 0.5-300 ng/mL [26] Electrochemical Immunosensor 2 pg/mL 0.01-1000 ng/mL [27] Electrochemical aptasensor 3.4 pg/mL 0.01-50 ng/mL [28] Electrochemical aptasensor 10 pg/mL 10 -11 -10 -4 g/mL [29] CV aptasensor 0.14 ng/mL 0.5-500 ng/mL This work Naghshbandi et al Journal of Nanobiotechnology (2022) 20:534 repeatability with a relative standard deviation (RSD) of 2.21% and 1.87%, respectively. As can be seen in Fig.…”

Section: Reproducibility Repeatability and Stability Of The Designed ...mentioning

confidence: 99%

Design of a new electrochemical aptasensor based on screen printed carbon electrode modified with gold nanoparticles for the detection of fumonisin B1 in maize flour

et al. 2023

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A new aptasensor for detecting fumonisin B1 (FB1) in the maize samples was developed based on DNA- aptamer recognition and electrochemical technique. A thiol-modified single-stranded DNA (ss-HSDNA) was immobilized on a screen printed carbon electrode (SPCE) electrodeposited by gold nanoparticles (AuNPs). The morphology and structure of SPCE and AuNPs/SPCE were evaluated via scanning electron microscopy (SEM) equipped with energy dispersive spectroscopy (EDS). The SEM results demonstrated that the SPCE had a flat sheet-like structure, and the AuNPs were homogeneously electrodeposited on the SPCE. Cyclic voltammetry (CV) experiments in the [Fe(CN)6]− 3/− 4 solution were conducted to investigate each step of electrode modification as well as aptasensor performance. Aptamer-FB1 interaction prevented the electron transfer permitting the determination of FB1 in the range of 0.5–500 ng/mL with a low detection limit (0.14 ng/mL). The designed aptasensor was also shown high selectivity, acceptable repeatability and reproducibility, good long-term stability, and excellent recovery. Furthermore, there was a strong correlation between the findings achieved via the designed aptasensor and high performance liquid chromatography (HPLC). Therefore, a simple construction process and satisfactory electrochemical performance of the proposed aptasensor have a great potential for the detection of FB1 in maize samples.

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“…As a classical natural enzyme, HRP with high catalytic ability is of great interest in CM biosensors for acquiring strong responses. For example, Tao et al (2020) developed a CM aptasensor for FB 1 detection through the competitive binding of the cDNA-HRP sensing probes and the added FB 1 to the biotin-labeled FB 1 aptamers immobilized on a microplate. Visual detection was realized with a low LOD of 0.3 ng/ml for FB 1 .…”

Section: Aptasensorsmentioning

confidence: 99%

Aptasensors for mycotoxins in foods: Recent advances and future trends

Hou

Jia

Sheng

et al. 2021

Comp Rev Food Sci Food Safe

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Mycotoxin contamination in foods has posed serious threat to public health and raised worldwide concern. The development of simple, rapid, facile, and cost‐effective methods for mycotoxin detection is of urgent need. Aptamer‐based sensors, abbreviated as aptasensors, with excellent recognition capacity to a wide variety of mycotoxins have attracted ever‐increasing interest of researchers because of their simple fabrication, rapid response, high sensitivity, low cost, and easy adaptability for in situ measurement. The past few decades have witnessed the rapid advances of aptasensors for mycotoxin detection in foods. Therefore, this review first summarizes the reported aptamer sequences specific for mycotoxins. Then, the recent 5‐year advancements in various newly developed aptasensors, which, according to the signal output mode, are divided into electrochemical, optical and photoelectrochemical categories, for mycotoxin detection are comprehensively discussed. A special attention is taken on their strengths and limitations in real‐world application. Finally, the current challenges and future perspectives for developing novel highly reliable aptasensors for mycotoxin detection are highlighted, which is expected to provide powerful references for their thorough research and extended applications. Owing to their unique advantages, aptasensors display a fascinating prospect in food field for safety inspection and risk assessment.

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Competitive HRP-Linked Colorimetric Aptasensor for the Detection of Fumonisin B1 in Food based on Dual Biotin-Streptavidin Interaction

Cited by 24 publications

References 41 publications

Investigation of the Influence of Liquid Motion in a Flow-based System on an Enzyme Aggregation State with an Atomic Force Microscopy Sensor: The Effect of Water Flow

Investigation of the Influence of Liquid Motion in a Flow-based System on an Enzyme Aggregation State with an Atomic Force Microscopy Sensor: The Effect of Water Flow

Design of a new electrochemical aptasensor based on screen printed carbon electrode modified with gold nanoparticles for the detection of fumonisin B1 in maize flour

Aptasensors for mycotoxins in foods: Recent advances and future trends

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