Competitive metal‐binding stoichiometry between calcium and strontium by cell wall proteins of Neurospora crassa

Abstract

Cell wall proteins from Neurospora crassa were isolated and evaluated to demonstrate their metal ability to bind Ca 2+ /Sr 2+ by loading the solubilized protein fraction on to immobilized metal affinity chromatography (IMAC) column pre-equilibrated with Ca 2+ /Sr 2+ . The sodium dodecyl sulfatepolyacrylamide gel electrophoresis analysis IMAC eluent, revealed ∼18 proteins with a similarity in the proteome pattern of Ca 2+ /Sr 2+ fractions.Diethyl aminoethyl chromatography showed five proteins in common in binding to Ca 2+ and Sr 2+ , were subjected to N-terminal sequencing. The sequence analysis was studied for the determination of metal-binding site prediction by CHED software indicating that all five were found to have a high affinity toward Ca 2+ . From these five, two were randomly selected and denoted as CWP-A (possess five Ca binding sites of six metal-binding sites) and CWP-B (possess six binding sites of eight metal-binding sites). They were selected for further characterization studies to determine their Ca 2+ bound Sr 2+ binding properties. Surprisingly, these proteins were able to bind Sr 2+ ions (29 μmol) with equal affinity as to Ca 2+ ions (42 μmol) by means of direct binding, and/or by displacing calcium as observed in metal-dependent proteolytic protection, fluorescence-based metal exchange assays, and molecular simulation studies. From the results, we demonstrate for the first time, that there is a stoichiometry between Ca 2+ (an essential macro elemental metal ion) and Sr 2+ ions (a nonessential element for which no reported metabolic activity is reported) for the metal-binding sites on cell wall proteins.