Competitive protein binding assay for biotin monitored by chemiluminescence

Abstract

monochromator slit width. (Increasing the lamp current would also reduce UBE.) However, in none of the cases investigated would this produce a significant reduction in the total absorbance noise. (Even if up were reduced to zero, OA would not be reduced by more than 30% in any case.) In addition, increasing the lamp current or slit width would lead to a reduction of the absorbance signal in many cases and consequently have a detrimental effect on the detection limit.Lamp flicker noise can be reduced by using a double beam system to monitor changes in lamp intensity. However, if the lamp duty cycle is unchanged, the double beam system necessitates halving the time of observation of the lamp. This

Diagnostics on acute myocardial infarction: Cardiac troponin biomarkers

“…To label proteins in CLIA, the most commonly used labeling enzymes are horseradish peroxidase (HRP) and ALP. CL has been used as a label in immunoassay by Schroeder et al (1976), who first described a method for monitoring competitive protein binding reactions by using CL.…”

Chemiluminescence immunoassay

“…Since the first report on CL labels in 1976 [31], considerable efforts have been devoted to developing practical CL-labeling systems because of their low limits of detection (LODs) [32]. Luminol, isoluminol and its derivatives, acridinium ester, horseradish peroxidase (HRP) and alkaline phosphatase (ALP) have frequently been employed as CL labels in IA for development and application of CLIA methods.…”

Enzyme Immunoassay: A Review

“…The sensitivity obtained (2 nM) was sufficient to measure oestriol from plasma samples (Kohen et aI., 1978). This method may be subject to interference by endogenous cofactors and degrading enzymes found in biological materials (Schroeder et al, 1976). If these problems can be overcome then this type of assay may be a useful addition to immunoassay methods.…”

“…Non-isotopic labels used include bacteriophages (Haimovich et al, 1970), erythrocytes (Alder and Chi-Tan, 1971), stable free radicals (Leute et al, 1972), chemiluminescent labels (Schroeder et al, 1976), fluorescent groups (Shaw et al, 1977;Ullman et al, 1976;Miller, 1979) and enzymes.…”