Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I

Rodríguez-Mallón, Alina; Cárdenas, Yamilet; Lugo, Juana María; Oliva, Aymé; Morales, Antonio; Estrada, Mario Pablo

doi:10.1007/s12575-009-9002-3

Cited by 5 publications

(2 citation statements)

References 24 publications

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“…Both clades of teleost GHRs are class I helical cytokine receptors in group 1, which signal via formation of a homodimer and are characterized by the presence of an extracellular cytokine receptor homology domain without additional immunoglobin-like or fibronectin domains [34]. Both GHR1 clade and GHR2 clade receptors are highly expressed in the liver in tilapia [22,35,47,53], as in other teleost species studied to date [11,20,29,34,54,64].…”

Section: Introductionmentioning

confidence: 99%

Regulation of growth hormone (GH) receptor (GHR1 and GHR2) mRNA level by GH and metabolic hormones in primary cultured tilapia hepatocytes

Pierce

Breves

Moriyama

et al. 2012

General and Comparative Endocrinology

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Section: Introductionmentioning

confidence: 99%

Regulation of growth hormone (GH) receptor (GHR1 and GHR2) mRNA level by GH and metabolic hormones in primary cultured tilapia hepatocytes

Pierce

Breves

Moriyama

et al. 2012

General and Comparative Endocrinology

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“…Thus, the most sensitive and reproducible technique for performing quantitative PCR in an ‘endpoint’ format is the competitive PCR method that has been used for a wide range of applications. However, this method is labor‐intensive and requires results from multiple reactions for each sample to be analyzed 3…”

Section: Detection Of Rna By Real‐time Rt‐pcrmentioning

confidence: 99%

RNA diagnostics: real‐time RT‐PCR strategies and promising novel target RNAs

2010

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Ribonucleic acid (RNA) is a multifunctional type of molecule, playing critical roles in protein biosynthesis and regulation. In recent years, suppression of protein translation by so-called microRNAs came into the focus of research, especially because deregulation of this process has been shown to play a role in malignant transformation. Furthermore, RNA molecules circulating in the blood have been revealed as a novel class of markers for diagnosis of cancers. Moreover, genetic information of some pathogens is stored as RNA, allowing their sensitive detection using nucleic acid amplification techniques. In this article, the principle of detecting different RNA types by real-time reverse-transcription polymerase chain reaction applications is described. Furthermore, the emerging use of microRNA and circulating RNA profiles complementing the broad spectrum of RNA diagnosis is discussed.

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Molecular dissection of a rice microtubule-associated RING finger protein and its potential role in salt tolerance in Arabidopsis

et al. 2015

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Although a number of RING E3 ligases in plants have been demonstrated to play key roles in a wide range of abiotic stresses, relatively few studies have detailed how RING E3 ligases exert their cellular actions. We describe Oryza sativa RING finger protein with microtubule-targeting domain 1 (OsRMT1), a functional RING E3 ligase that is likely involved in a salt tolerance mechanism. Functional characterization revealed that OsRMT1 undergoes homodimer formation and subsequently autoubiquitination-mediated protein degradation under normal conditions. By contrast, OsRMT1 is predominantly found in the nucleus and microtubules and its degradation is inhibited under salt stress. Domain dissection of OsRMT1 indicates that the N-terminal domain is required for microtubule targeting. Bimolecular fluorescence complementation analysis and degradation assay revealed that OsRMT1-interacted proteins localized in various organelles were degraded via the ubiquitin (Ub)/26S proteasome-dependent pathway. Interestingly, when OsRMT1 and its target proteins were co-expressed in N. benthamiana leaves, the protein-protein interactions appeared to take place mainly in the microtubules. Overexpression of OsRMT1 in Arabidopsis resulted in increased tolerance to salt stress. Our findings suggest that the abundance of microtubule-associated OsRMT1 is strictly regulated, and OsRMT1 may play a relevant role in salt stress response by modulating levels of its target proteins.

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Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I

Cited by 5 publications

References 24 publications

Regulation of growth hormone (GH) receptor (GHR1 and GHR2) mRNA level by GH and metabolic hormones in primary cultured tilapia hepatocytes

Regulation of growth hormone (GH) receptor (GHR1 and GHR2) mRNA level by GH and metabolic hormones in primary cultured tilapia hepatocytes

RNA diagnostics: real‐time RT‐PCR strategies and promising novel target RNAs

Molecular dissection of a rice microtubule-associated RING finger protein and its potential role in salt tolerance in Arabidopsis

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