The Mesoproterozoic Frontenac terrane in the southern Grenville Province of Ontario is separated by shear zones from the Composite Arc Belt to the west and the Adirondack Lowlands to the east. The majority of the terrane is made up of granulite-facies metasedimentary rocks that are the oldest lithologies recognized in the terrane. Five samples were selected for U-Pb geochronology to constrain (1) metamorphic age, (2) depositional age of sedimentary protoliths, and (3) source regions of detrital zircon. Two pelitic gneisses and a marble are dominated by metamorphic zircon, whereas two quartzites contain a diverse population of detrital zircon that are surrounded by metamorphic rims. Metamorphic zircon have 206Pb/207Pb ages of 1.19–1.16 Ga, and a small population is 1.22 Ga. These ages correspond to the Shawinigan and Elzevirian orogenies, and provide minimum ages for deposition. The youngest detrital grains with ages of 1.25–1.24 Ga provide maximum depositional ages. Quartzites (and pelitic gneisses) have a wide range of detrital zircon ages that reflect local Mesoproterozoic Grenville sources and Paleoproterzoic and Archean sources in the northern Grenville Province and elsewhere in Laurentia. Most notable is a large population of 1.9–1.8 Ga zircon which point towards derivation from the Penokean orogen in the Midcontinent or Makkovikian–Ketilidian orogen of Labrador and Greenland; indicating long sedimentary transport distances. The similarities in depositional ages and detrital zircon ages between Frontenac terrane and Adirondack metasedimentary rocks suggest a shared sedimentary history which we interpret as deposition in the same Trans-Adirondack backarc basin at ca. 1.25 Ga.