Complementary combination of biomarkers for diagnosis of sarcopenia in C57BL/6J mice

Long, Nguyen Van; Chien, Pham Ngoc; Tung, Trinh Xuan; Anh, Le Thi Van; Giang, Nguyen Ngan; Nga, Pham Thi Quynh; Linh, Le Thi Thuy; Nam, Sun‐Young; Heo, Chan Yeong

doi:10.1016/j.lfs.2022.121213

Cited by 6 publications

(4 citation statements)

References 58 publications

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“…This could have significant implications for the establishment of new proteomic biomarker signatures of aging. 78 , 79 , 100 , 101 Hence, the new MS-based reference map could be useful for detailed future evaluations of (i) muscle fiber type shifting, 33 , 66 , 102 (ii) metabolic disturbances and alterations of the gut-muscle axis, 103 (iii) mitochondrial dysfunction, inflammation and impaired cellular signaling, 84 and (iv) protein modifications, 6 as well as the potential influence of (v) pharmacological therapies and related treatments, 74 , 75 , 104 (vi) physical activity levels, 105 , 106 (vii) nutritional interventions, 104 , 107-109 and (viii) gender specific differences, 110 on the development of different forms of sarcopenia. 71-73 Hence, novel proteomic biomarkers could be beneficial for better defining and differentiating the presarcopenic loss of skeletal muscle mass and decline in contractile function, 111 , 112 from acute versus chronic forms of sarcopenia of old age.…”

Section: Discussionmentioning

confidence: 99%

“…Animal models of aging play a critical role in sarcopenia research. 100 , 115 , 116 This report has outlined the mass spectrometric analysis of total protein extracts from crude muscle tissue extracts, which has resulted in the establishment of a proteomic reference map of the aged mouse hindlimb musculature. Figure 2 gives an overview of identified protein groupings in senescent skeletal muscles using bottom-up proteomics.…”

Section: Discussionmentioning

confidence: 99%

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Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins of organelles, cellular signaling, bioenergetic metabolism and molecular chaperoning

Dowling,

Gargan,

Zweyer

et al. 2024

Eur J Transl Myol

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During the natural aging process, frailty is often associated with abnormal muscular performance. Although inter-individual differences exit, in most elderly the tissue mass and physiological functionality of voluntary muscles drastically decreases. In order to study age-related contractile decline, animal model research is of central importance in the field of biogerontology. Here we have analyzed wild type mouse muscle to establish a proteomic map of crude tissue extracts. Proteomics is an advanced and large-scale biochemical method that attempts to identify all accessible proteins in a given biological sample. It is a technology-driven approach that uses mass spectrometry for the characterization of individual protein species. Total protein extracts were used in this study in order to minimize the potential introduction of artefacts due to excess subcellular fractionation procedures. In this report, the proteomic survey of aged muscles has focused on organellar marker proteins, as well as proteins that are involved in cellular signaling, the regulation of ion homeostasis, bioenergetic metabolism and molecular chaperoning. Hence, this study has establish a proteomic reference map of a highly suitable model system for future aging research.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins of organelles, cellular signaling, bioenergetic metabolism and molecular chaperoning

Dowling,

Gargan,

Zweyer

et al. 2024

Eur J Transl Myol

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“…This gives non-invasive biomarker investigations of biological fluids, such as serum, saliva or urine, a central role in aging research [501][502][503]. The serum of both older humans suffering from sarcopenia [504][505][506][507] and senescent mice [508] were shown to exhibit differential changes in common markers that are associated with inflammation, remodeling of the extracellular matrix and mitochondrial functions [398]. This suggests the potential usage of pro-inflammatory cytokines, growth factors, differentiation factors and leaked mitochondrial proteins as suitable biofluid markers to evaluate the degree of skeletal muscle aging [400].…”

Section: Biomarker Discovery For the Improved Evaluation Of Sarcopeni...mentioning

confidence: 99%

Fiber-Type Shifting in Sarcopenia of Old Age: Proteomic Profiling of the Contractile Apparatus of Skeletal Muscles

Dowling

Gargan

Swandulla

et al. 2023

IJMS

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The progressive loss of skeletal muscle mass and concomitant reduction in contractile strength plays a central role in frailty syndrome. Age-related neuronal impairments are closely associated with sarcopenia in the elderly, which is characterized by severe muscular atrophy that can considerably lessen the overall quality of life at old age. Mass-spectrometry-based proteomic surveys of senescent human skeletal muscles, as well as animal models of sarcopenia, have decisively improved our understanding of the molecular and cellular consequences of muscular atrophy and associated fiber-type shifting during aging. This review outlines the mass spectrometric identification of proteome-wide changes in atrophying skeletal muscles, with a focus on contractile proteins as potential markers of changes in fiber-type distribution patterns. The observed trend of fast-to-slow transitions in individual human skeletal muscles during the aging process is most likely linked to a preferential susceptibility of fast-twitching muscle fibers to muscular atrophy. Studies with senescent animal models, including mostly aged rodent skeletal muscles, have confirmed fiber-type shifting. The proteomic analysis of fast versus slow isoforms of key contractile proteins, such as myosin heavy chains, myosin light chains, actins, troponins and tropomyosins, suggests them as suitable bioanalytical tools of fiber-type transitions during aging.

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“…Adipokines released by adipose tissue produce important modifications during aging and can affect muscle metabolism and physiology. Therewithal, catabolic hormones such as cortisol and angiotensin II can accelerate age-induced muscle atrophy as they are involved in muscle wasting, and their levels increase with age [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Insights into Pathogenesis, Nutritional and Drug Approach in Sarcopenia: A Systematic Review

et al. 2023

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Sarcopenia is a multifactorial condition related to the loss of muscle mass and strength due to aging, eating habits, physical inactivity, or even caused by another disease. Affected individuals have a higher risk of falls and may be associated with heart disease, respiratory diseases, cognitive impairment, and consequently an increased risk of hospitalization, in addition to causing an economic impact due to the high cost of care during the stay in hospitals. The standardization of appropriate treatment for patients with sarcopenia that could help reduce pathology-related morbidity is necessary. For these reasons, this study aimed to perform a systematic review of the role of nutrition and drugs that could ameliorate the health and quality of life of sarcopenic patients and PRISMA guidelines were followed. Lifestyle interventions have shown a profound impact on sarcopenia treatment but using supplements and different drugs can also impact skeletal muscle maintenance. Creatine, leucine, branched-chain amino acids, omega 3, and vitamin D can show benefits. Although with controversial results, medications such as Metformin, GLP-1, losartan, statin, growth hormone, and dipeptidyl peptidase 4 inhibitors have also been considered and can alter the sarcopenic’s metabolic parameters, protect against cardiovascular diseases and outcomes, while protecting muscles.

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Complementary combination of biomarkers for diagnosis of sarcopenia in C57BL/6J mice

Cited by 6 publications

References 58 publications

Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins of organelles, cellular signaling, bioenergetic metabolism and molecular chaperoning

Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins of organelles, cellular signaling, bioenergetic metabolism and molecular chaperoning

Fiber-Type Shifting in Sarcopenia of Old Age: Proteomic Profiling of the Contractile Apparatus of Skeletal Muscles

Insights into Pathogenesis, Nutritional and Drug Approach in Sarcopenia: A Systematic Review

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