Complementary floral homeotic phenotypes result from opposite orientations of a transposon at the plena locus of antirrhinum

Bradley, Desmond; Carpenter, Rosemary; Sommer, Hans; Hartley, Nigel M.; Coen, Enrico

doi:10.1016/0092-8674(93)90052-r

Cited by 472 publications

(448 citation statements)

References 31 publications

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“…Methods used for digoxigenin labeling of mRNA probes, tissue preparation, and in situ hybridization were previously described (Bradley et al, 1993) but with small modifications. Instead of Pronase, protease treatment was performed with proteinase K (1 mg/mL in 100 mM Tris, pH 8, and 50 mM EDTA) at 37°C for 30 min.…”

Section: In Situ Hybridizationsmentioning

confidence: 99%

SWP73 Subunits of Arabidopsis SWI/SNF Chromatin Remodeling Complexes Play Distinct Roles in Leaf and Flower Development

et al. 2015

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(T.J.S.)Arabidopsis thaliana SWP73A and SWP73B are homologs of mammalian BRAHMA-associated factors (BAF60s) that tether SWITCH/SUCROSE NONFERMENTING chromatin remodeling complexes to transcription factors of genes regulating various cell differentiation pathways. Here, we show that Arabidopsis thaliana SWP73s modulate several important developmental pathways. While undergoing normal vegetative development, swp73a mutants display reduced expression of FLOWERING LOCUS C and early flowering in short days. By contrast, swp73b mutants are characterized by retarded growth, severe defects in leaf and flower development, delayed flowering, and male sterility. MNase-Seq, transcript profiling, and ChIP-Seq studies demonstrate that SWP73B binds the promoters of ASYMMETRIC LEAVES1 and 2, KANADI1 and 3, and YABBY2, 3, and 5 genes, which regulate leaf development and show coordinately altered transcription in swp73b plants. Lack of SWP73B alters the expression patterns of APETALA1, APETALA3, and the MADS box gene AGL24, whereas other floral organ identity genes show reduced expression correlating with defects in flower development. Consistently, SWP73B binds to the promoter regions of APETALA1 and 3, SEPALLATA3, LEAFY, UNUSUAL FLORAL ORGANS, TERMINAL FLOWER1, AGAMOUS-LIKE24, and SUPPRESSOR OF CONSTANS OVEREXPRESSION1 genes, and the swp73b mutation alters nucleosome occupancy on most of these loci. In conclusion, SWP73B acts as important modulator of major developmental pathways, while SWP73A functions in flowering time control.

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Section: In Situ Hybridizationsmentioning

confidence: 99%

SWP73 Subunits of Arabidopsis SWI/SNF Chromatin Remodeling Complexes Play Distinct Roles in Leaf and Flower Development

et al. 2015

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“…The methods used for digoxygenin labelling of RNA probes, tissue preparation and in situ hybridization were as described by Jackson (1991) and Bradley et al (1993). Probes were made from bZIP910 and bZIP911 cDNA fragments subcloned in pBluescript SKϩ.…”

Section: In Situ Hybridizationmentioning

confidence: 99%

Two bZIP proteins from Antirrhinum flowers preferentially bind a hybrid C‐box/G‐box motif and help to define a new sub‐family of bZIP transcription factors

et al. 1998

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SummaryTwo genes encoding bZIP proteins are expressed in flowers of Antirrhinum majus, predominantly in vascular tissues, carpels and anthers. The sequences of cDNA clones encoding these proteins show them to belong to a distinct subfamily of bZIP proteins which also includes LIP19 from rice and MLIPI5 and OBF1 from maize. The sub-family is characterized by the inclusion of very small proteins consisting of essentially a basic domain and a long leucine zipper. Members also have a conserved upstream open reading frame (uORF) in their 5Ј leader sequences, implying a common mode of post-transcriptional control. In vitro, the Antirrhinum bZIP proteins preferentially bind to a novel hybrid C-box/G-box motif which is found in the promoters of some plant histone genes and of some nuclear-encoded genes with plastidial protein products. Expression of the bZIP proteins in transgenic tobacco under control of the CaMV 35S promoter supports the view that they can regulate expression of genes which contain the preferred target motif within their regulatory sequences, although both enhancement and repression of transcript levels of target genes were observed, indicating that the bZIP proteins probably interact with other factors to modulate transcription in different ways, as has been observed for the small MAF family of bZIP proteins in vertebrates.

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“…In Arabidopsis and Antirrhinum, genes class A, B and C have been identi®ed (Yanofsky et al 1990;Sommer et al 1990;Jack et al 1992;Mandel et al 1992;Trobner et al 1992;Bradley et al 1993;Goto & Meyerowitz 1994;Jofuku et al 1994). Most of these genes encode proteins with a conserved DNA-binding domain, the MADS box.…”

Section: Introductionmentioning

confidence: 99%

The whorl‐specific action of a petunia class B floral homeotic gene

et al. 2000

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Background: GREEN PETAL (GP) is thought to be a petunia class B¯oral homeotic gene, because the gp mutant¯ower displays a severe homeotic conversion of petals into sepals in the second whorl. However, since the third whorl stamens remain unaffected in the gp null mutant, gp is different from class B mutants in Arabidopsis and Antirrhinum, which also show a conversion of the third whorl stamens into the carpelloid tissue. BLIND (BL) is thought to be a petunia class A¯oral homeotic gene, because the bl mutant¯ower displays homeotic conversions of sepals into the stigmatoid tissue in the ®rst whorl and of the corolla limb into antheroid structures in the second whorl.

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Complementary floral homeotic phenotypes result from opposite orientations of a transposon at the plena locus of antirrhinum

Cited by 472 publications

References 31 publications

SWP73 Subunits of Arabidopsis SWI/SNF Chromatin Remodeling Complexes Play Distinct Roles in Leaf and Flower Development

SWP73 Subunits of Arabidopsis SWI/SNF Chromatin Remodeling Complexes Play Distinct Roles in Leaf and Flower Development

Two bZIP proteins from Antirrhinum flowers preferentially bind a hybrid C‐box/G‐box motif and help to define a new sub‐family of bZIP transcription factors

The whorl‐specific action of a petunia class B floral homeotic gene

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