2024
DOI: 10.21203/rs.3.rs-5088576/v1
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Complementary insights into gut viral genomes: a comparative benchmark of short- and long-Read metagenomes using diverse assemblers and binners

Huarui Wang,
Chuqing Sun,
Yun Li
et al.

Abstract: Background Metagenome-assembled viral genomes have significantly advanced the discovery and characterization of the human gut virome. However, we lack a comparative assessment of assembly tools on the efficacy of viral genome identification, particularly across Next Generation Sequencing (NGS) and Third Generation Sequencing (TGS) data. Results We evaluated the efficiency of NGS, TGS and hybrid assemblers for viral genome discovery using 95 viral-like particle (VLP) enriched fecal samples sequenced on both I… Show more

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