From global to local scales, human-induced environmental changes can impact mountain lakes by, for example, altering species composition, trophic state, and thermal dynamics, thereby affecting ecosystem functions and processes. However, the consequences of these changes on ecosystem services (ES) of mountain lakes are unclear due to a lack of integrative assessments. Therefore, this dissertation adopts an interdisciplinary ES perspective to improve insight into human interactions with mountain lakes and the potential social and ecological impacts of anthropogenic pressures on them. Stakeholder consultations highlighted the importance of regulating, cultural, and provisioning ES of natural mountain lakes, emphasizing habitat, aesthetics, recreation, education and research, and surface water, with nature-based experiences as being a key aspect of human interaction with mountain lakes. Multiple indicators were proposed to quantify potential supply of these ES, revealing variations among case study lakes in the European Alps. These results informed an integrative valuation approach and exposure assessment to anthropogenic pressures, suggesting that ES by mountain lakes are sensitive to climate change-related and human use pressures. Overall, the findings advance a social-ecological understanding of mountain lakes and indicate towards the need for adaptive lake management to maintain ES under potential anthropogenic pressure.