1973
DOI: 10.1093/genetics/74.4.581
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COMPLEMENTATION ANALYSIS OF METABOLITE-RESISTANT MUTATIONS WITH FORCED HETEROKARYONS OF NEUROSPORA CRASSA

Abstract: The double mutant strain pyr-3  arg-12s is a prototroph because a common precursor of arginine and pyrimidine is supplied by the arginine pathway. Growth of this strain is inhibited by exogenous citrulline or arginine. Citrulline-resistant mutants of this strain were selected, and they resulted from modifier mutations at other loci. Forced heterokaryons were used to study complementation among these modifiers. Since the complementation test requires the scoring of non-growth as the positive result, there was c… Show more

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Cited by 5 publications
(3 citation statements)
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“…Leaky arg-l mutants are frequent among those selected as citrulline-resistant variants of arg-12s; pyr-3. Most of these show interallelic complementation, and many are transport deficient (1075). arg-l mutants do not grow well on some complex complete media unless extra arginine is added.…”
Section: Arg: Argininementioning
confidence: 99%
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“…Leaky arg-l mutants are frequent among those selected as citrulline-resistant variants of arg-12s; pyr-3. Most of these show interallelic complementation, and many are transport deficient (1075). arg-l mutants do not grow well on some complex complete media unless extra arginine is added.…”
Section: Arg: Argininementioning
confidence: 99%
“…Several heat-sensitive genes have been sufficiently characterized to be assigned more specific names than un (e.g., ndc, rip, psi, eth-1, and fs-2). At least some un mutants are deficient in amino acid transport (543,1075). For most un loci, however, little is known of the molecular or cellular basis.…”
Section: (S04)mentioning
confidence: 99%
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