Complete and coreless hemagglutinating particles produced in ECHO 12 virus-infected cells

Halperen, S.; Eggers, Hans J.; Tamm, Igor

doi:10.1016/s0042-6822(64)80010-6

Cited by 33 publications

(7 citation statements)

References 15 publications

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“…The protein capsids of the picornaviruses studied reach equilibrium at a density of 1.29 g/cc in CsCl density gradients. This value for the buoyant density of picornavirus capsids in CsCI confirms JAMISON AND MAYOR the work of Halperen et al (6) and Fabiyi et al (4).…”

Section: Discussionsupporting

confidence: 88%

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Comparative Study of Seven Picornaviruses of Man

Jamison

Mayor

1966

J Bacteriol

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The buoyant densities in CsCl and potassium tartrate of seven acid-resistant picornaviruses of man were determined. The viruses studied were found to be a relatively homogeneous group. Buoyant densities ranged from 1.32 to 1.35 g/cc in CsCl and 1.21 to 1.23 g/cc in potassium tartrate. The buoyant density of each virus did not vary by more than 0.01 g/cc. The host cell of origin and passage level did not influence the buoyant density of the virions. However, measurements of the diameters of purified virions by a standard method have shown that there are considerable size variations among these picornaviruses. The diameters ranged from 18 to 25 m,u. Echovirus 19 and poliovirus 1 were significantly larger (mean diameter, 23.5 m,u) than echoviruses 4, 6, 16, and 23, and coxsackievirus B2 (mean diameter, 21.0 m,u).

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Section: Discussionsupporting

confidence: 88%

“…Members of the group have been reported as ranging in size from 7 to 39 m,u (6,12). In an attempt to study the size variation existent in the group, we have determined the diameter of several of the picornaviruses by a standard method.…”

mentioning

confidence: 99%

Comparative Study of Seven Picornaviruses of Man

Jamison

Mayor

1966

J Bacteriol

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“…Similar results have been reported in echovirus type 12 (Halperen et al 1964) and foot-and-mouth disease virus (Rowlands et at. I975), However, the antigenicity of the procapsids was found to be more heatlabile than the virion.…”

Section: Discussionsupporting

confidence: 88%

“…The native procapsid consists of three polypeptides, VPo, I and 3-It shows N-antigenicity (Halperen et al 1964;Rowlands et al I975), H-antigenicity (Roizman et al 1958) or both N-and H-antigenicity (Lonberg-Holm & Yin, 1973 according to the species of virus. However, little is known of the correlation of antigenicity with the polypeptide composition of the heated procapsid.…”

Section: Introductionmentioning

confidence: 99%

Antigenicity and Polypeptide Composition of Native and Heated Echovirus Type 7 Procapsids

Hasegawa

Inouye

1979

Journal of General Virology

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SUMMARYThe native procapsid (naturally occurring empty capsid) of echovirus type 7 (E7) possesses N-antigenicity, which is as highly strain-specific as the native virion. When the native procapsid is heated, its antigenicity is converted to H-anligenicity which is common among strains of E7 and thus type-specific. However, no difference was detected in the sedimentation rate (8oS) and polypeptide composition (VPo, 1 and 3) of the native and heated procapsids.

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“…With no template present, the particles would have no demonstrable DNA polymerase activity irrespective of whether the enzyme were present. The existence of such particles would certainly not be unexpected since most virus preparations do contain variable numbers of empty particles (10,11,15,25).…”

Section: Resultsmentioning

confidence: 99%

Demonstration of subpopulations of Dane particles

Kaplan¹,

Ford²,

Purcell³

et al. 1976

J Virol

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Two populations of Dane particles were isolated from the plasma of individuals carrying hepatitis B surface antigen. These populations had densities in CsCl of 1.22 and 1.20 g/ml. Endogenous DNA polymerase activity was found to be associated only with the heavier of these two populations. Using a positive stain, electron microscopic examination of these particles suggested that the heavier the particle contained nucleic acid in its core whereas the lighter particle appeared empty. Cores isolated from Dane particles with densities of 1.22 and 1.20 g/ml banded in CsCl at densities of 1.36 and 1.30 g/ml, respectively. Endogenous DNA polymerase activity was associated only with the higher density core particles.

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Complete and coreless hemagglutinating particles produced in ECHO 12 virus-infected cells

Cited by 33 publications

References 15 publications

Comparative Study of Seven Picornaviruses of Man

Comparative Study of Seven Picornaviruses of Man

Antigenicity and Polypeptide Composition of Native and Heated Echovirus Type 7 Procapsids

Demonstration of subpopulations of Dane particles

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