Complete cDNA Sequences and Phylogenetic Analyses of the Th1 and Th2 Cytokines of the Bactrian Camel (Camelus bacterianus)

Odbileg, Raadan; Purevtseren, Byambaa; Batsukh, Zayat; Konnai, Satoru; Ohashi, Kazuhiko; Onuma, Misao

doi:10.1292/jvms.68.941

Cited by 11 publications

(8 citation statements)

References 25 publications

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“…Mature goat amino acid sequence has two N-glycosylation sites Asn 16 -x-Ser 18 and Asn 83 -x-Ser 85 (Fig. 2) consistent with those observed in other species (10,23,24). However, glycosylation of IFN-c has been observed to be unimportant for functional activity (25).…”

Section: Ifn-csupporting

confidence: 68%

Molecular Cloning, Sequence, and Phylogenetic Analysis of T Helper 1 Cytokines of Pashmina Goats

Shebannavar

Rasool²

2014

Animal Biotechnology

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Cytokines play an important role in regulation of immune responses either in health or disease. In the present study, the cDNAs encoding mature Interleukin (IL)-2, interferon gamma (IFN-γ), and IL-12 p35 and p40 of Pashmina goat were cloned and sequenced. The amino acid sequence was deduced from nucleotide sequence and compared with those available in GeneBank. Mature forms of goat IL-2, IFN-γ, IL-12 p35, and IL-12 p40 composed of 135, 143, 196, and 305 amino acid residues, respectively. Comparison of amino acid sequence of goat IL-2 with sheep, buffalo, cattle, pig, camel, cat, and human sequences showed homology percentages of 100, 97.8, 96.3, 72.4, 72.4, 67.2, and 64.7, respectively. Amino acid sequence of goat IFN-γ showed 98.6, 95.8, 81.1, 81.8, 80.4, and 62.9 percent homology with sheep, bovine, pig, horse, dog, and humans, respectively. Homology ranging from 81.6 to 99% for IL-12 p35 sequences and 85.6 to 100% for IL-12 p40 sequences at amino acid level were observed across these species. Multiple sequence alignment and phylogenetic analysis of goat cytokines revealed close relationship with sheep sequence.

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Section: Ifn-csupporting

confidence: 68%

Molecular Cloning, Sequence, and Phylogenetic Analysis of T Helper 1 Cytokines of Pashmina Goats

Shebannavar

Rasool²

2014

Animal Biotechnology

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“…The characterization of T effector cell subsets is limited by the lack of monoclonal antibodies specific for camel Th1, Th2, and Th17 cytokines. The characterized genes of Th1 (IL-2, IL-12, and IFN-γ) and Th2 (IL-4, IL-10 and IL-13) cytokines in the Bactrian camel ( 137 , 138 ), however, could represent a valuable tool for conducting functional studies on T cell polarization in camels. The high homology between Bactrian camels and other species, including the llama, pig, cow, and horse regarding the nucleotide sequences of their cytokine genes ( 137 ) also suggests the necessity of testing monoclonal antibodies specific for cytokines of these species for their cross-reactivity with camel cytokines.…”

Section: Characterization Of Phenotype and Function Of Camel Leukocytmentioning

confidence: 99%

Recent Advances in Camel Immunology

Hussen

Schuberth

2021

Front. Immunol.

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Camels are domesticated animals that are highly adapted to the extreme desert ecosystem with relatively higher resistance to a wide range of pathogens compared to many other species from the same geographical region. Recently, there has been increased interest in the field of camel immunology. As the progress in the analysis of camel immunoglobulins has previously been covered in many recent reviews, this review intends to summarize published findings related to camel cellular immunology with a focus on the phenotype and functionality of camel leukocyte subpopulations. The review also describes the impact of different physiological (age and pregnancy) and pathological (e.g. infection) conditions on camel immune cells. Despite the progress achieved in the field of camel immunology, there are gaps in our complete understanding of the camel immune system. Questions remain regarding innate recognition mechanisms, the functional characterization of antigen-presenting cells, and the characterization of camel NK and cytotoxic T cells.

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“…Based on the approach of Odbileg et al (2005) and Odbileg, et al (2006) who recorded cross reactivity between camel and cow, horse, human, and mouse cytokines, IL-1β and IL-10, TNF-α, IFN-γ, IL-6 and IL-1α levels were determined from undiluted serum samples using commercially available ELISA Kits (Biosurce, Diagnostic Corporation, Belgium). The plates were read at 450 nm and a correction wavelength of 550 nm was measured on a computerized automated microplate ELISA reader (Bio TEC, ELX800G, USA).…”

Section: Serological Testingmentioning

confidence: 99%

Cytokine response and clinicopathological findings in Brucella infected camels (Camelus dromedarius)

El‐Boshy¹,

Abbas²,

El-Khodery³

et al. 2009

Vet. Med. - Czech

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The present study had the aim of assessing the cytokine response and selected clinicopathological findings associated with brucellosis in camels (Camelus dromedarius). 340 dromedary camels were examined for brucellosis using agglutination and Complement Fixation tests (CFT). Twenty-five camels (7.35%) were positive by both tests; 14 (4.12%) for B. abortus and 11 (3.23%) for B. melitensis. IL-1β and IL-10 interleukin levels in both B. abortus and B. melitensis infected camels showed significant elevations (P < 0.05) compared with controls. Moreover, there was significantly larger increase in IL-1β interleukins in camels infected with B. abortus compared with B. melitensis. TNF-α, IFN-γ and IL-1α levels showed significant decreases (P < 0.05) in Brucella infected camels compared with non-infected ones; however, there was non-significant changes in IL-6 levels in Brucella infected camels compared with controls. Lymphopenia was recorded in infected camels but not in controls. However, normocytic normochromic anemia, hypoproteinemia, hypoalbuminemia and hypoglycemia were recorded in the B. abortus group only. Sorbitol dehydrogenase (SD), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) showed significant increases (P < 0.05) in infected camels compared with controls, and in B. abortus infected camels compared with B. melitensis infected animals. This is the first report that describes changes in selected cytokines and various haematological and biochemical parameters associated with brucellosios in dromedary camels. Emphasis should be placed on multidisciplinary research to elucidate the immunomodulatory features of camel brucellosis.

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Complete cDNA Sequences and Phylogenetic Analyses of the Th1 and Th2 Cytokines of the Bactrian Camel (Camelus bacterianus)

Cited by 11 publications

References 25 publications

Molecular Cloning, Sequence, and Phylogenetic Analysis of T Helper 1 Cytokines of Pashmina Goats

Molecular Cloning, Sequence, and Phylogenetic Analysis of T Helper 1 Cytokines of Pashmina Goats

Recent Advances in Camel Immunology

Cytokine response and clinicopathological findings in Brucella infected camels (Camelus dromedarius)

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