Complete genome analysis of <i>Vibrio mimicus</i> strain SCCF01, a highly virulent isolate from the freshwater catfish

Yu, Zehui; Wang, Erlong; Geng, Yi; Wang, Kaiyu; Chen, Defang; Huang, Xiaoli; Ouyang, Ping; Zuo, Zhuang; Huang, Chao; Fang, Jing; Yin, Lizi; Guo, Hongrui; Zhong, Zhijun

doi:10.1080/21505594.2019.1702797

Cited by 23 publications

(23 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The relatively high prevalence of typical Vibrio parahaemolyticus virulence genes in Vibrio alginolyticus in this study supports earlier reports that showed that most Vibrio spp. possesses atypical virulence genes in addition to their typical virulence determinants [ 79 ]. For example, the work of [ 80 ] detected V. cholerae ctxAB gene in V. diabolicu, V. alginolyticus and V. parahaemolyticus while studies carried out by [ 30 ] and [ 81 ] reported V. parahaemolyticus tdh gene in V. harveyi and V. mimicus respectively.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of virulence determinants in vibrio cholerae, vibrio mimicus, vibrio alginolyticus, and vibrio parahaemolyticus isolates from important water resources of Eastern Cape, South Africa

Abioye,

Osunla,

Nontongana

et al. 2023

BMC Microbiol

View full text Add to dashboard Cite

Background Virulence determinants are crucial to the risk assessment of pathogens in an environment. This study investigated the presence of eleven key virulence-associated genes in Vibrio cholerae (n = 111) and Vibrio mimicus (n = 22) and eight virulence determinants in Vibrio alginolyticus (n = 65) and Vibrio parahaemolyticus (n = 17) isolated from six important water resources in Eastern Cape, South Africa, using PCR techniques. The multiple virulence gene indexes (MVGI) for sampling sites and isolates as well as hotspots for potential vibriosis outbreaks among sampling sites were determined statistically based on the comparison of MVGI. Result The PCR assay showed that all the V. cholerae isolates belong to non-O1/non-O139 serogroups. Of the isolates, Vibrio Cholera (84%), V. mimicus (73%), V. alginolyticus (91%) and V. parahaemolyticus (100%) isolates harboured at least one of the virulence-associated genes investigated. The virulence gene combinations detected in isolates varied at sampling site and across sites. Typical virulence-associated determinants of V. cholerae were detected in V. mimicus while that of V. parahaemolyticus were detected in V. alginolyticus. The isolates with the highest MVGI were recovered from three estuaries (Sunday river, Swartkopps river, buffalo river) and a freshwater resource (Lashinton river). The cumulative MVGI for V. cholerae, V. mimicus, V. alginolyticus and V. parahaemolyticus isolates were 0.34, 0.20, 0.45, and 0.40 respectively. The targeted Vibrio spp. in increasing order of the public health risk posed in our study areas based on the MVGI is V. alginolyticus > V. parahaemolyticus > V. cholerae > V. mimicus. Five (sites SR, PA5, PA6, EL4 and EL6) out of the seventeen sampling sites were detected as the hotspots for potential cholera-like infection and vibriosis outbreaks. Conclusions Our findings suggest that humans having contact with water resources in our study areas are exposed to potential public health risks owing to the detection of virulent determinants in human pathogenic Vibrio spp. recovered from the water resources. The study affirms the relevancy of environmental Vibrio species to the epidemiology of vibriosis, cholera and cholera-like infections. Hence we suggest a monitoring program for human pathogenic Vibrio spp. in the environment most especially surface water that humans have contact with regularly.

show abstract

Section: Discussionmentioning

confidence: 99%

Occurrence of virulence determinants in vibrio cholerae, vibrio mimicus, vibrio alginolyticus, and vibrio parahaemolyticus isolates from important water resources of Eastern Cape, South Africa

Abioye,

Osunla,

Nontongana

et al. 2023

BMC Microbiol

View full text Add to dashboard Cite

show abstract

“…More virulence genes were found in the core-genome, with a higher number in ChI (511 genes) than in ChII (200 genes), the same pattern, but with lower values were observed in the accessory genome (233 genes in ChI and 221 genes in ChII). It has been reported that ChI has more plasticity [ 8 ] and more virulence genes than ChII. The genes present in ChI are responsible for growth and viability, while the genes in ChII are responsible for adaptation to environmental changes [ 1 , 6 ].…”

Section: Discussionmentioning

confidence: 99%

“…Vibrio mimicus has been recognized as an emergent pathogen in human diseases, and it has been isolated from cases of gastroenteritis, ear infections, and severe cholera-like diarrhoea, as well as from several marine products, aquatic plants, sediments, and water (marine, brackish, and freshwater) [ 3 , 4 , 5 ]. V. mimicus strains encode a wide variety of virulence factors, such as he, hemagglutinins, pili, metalloproteases, enterotoxins, and siderophores, which are mediated by several different mechanisms [ 5 , 6 , 7 , 8 ]. Moreover, studies of comparative genomics of Vibrio species have shown that genetic exchange among Vibrionaceae family species is a continuous process [ 6 , 9 , 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Core and Accessory Genome Analysis of Vibrio mimicus

et al. 2021

View full text Add to dashboard Cite

Vibrio mimicus is an emerging pathogen, mainly associated with contaminated seafood consumption. However, little is known about its evolution, biodiversity, and pathogenic potential. This study analyzes the pan-, core, and accessory genomes of nine V. mimicus strains. The core genome yielded 2424 genes in chromosome I (ChI) and 822 genes in chromosome II (ChII), with an accessory genome comprising an average of 10.9% of the whole genome for ChI and 29% for ChII. Core genome phylogenetic trees were obtained, and V. mimicus ATCC-33654 strain was the closest to the outgroup in both chromosomes. Additionally, a phylogenetic study of eight conserved genes (ftsZ, gapA, gyrB, topA, rpoA, recA, mreB, and pyrH), including Vibrio cholerae, Vibrio parilis, Vibrio metoecus, and Vibrio caribbenthicus, clearly showed clade differentiation. The main virulence genes found in ChI corresponded with type I secretion proteins, extracellular components, flagellar proteins, and potential regulators, while, in ChII, the main categories were type-I secretion proteins, chemotaxis proteins, and antibiotic resistance proteins. The accessory genome was characterized by the presence of mobile elements and toxin encoding genes in both chromosomes. Based on the genome atlas, it was possible to characterize differential regions between strains. The pan-genome of V. mimicus encompassed 3539 genes for ChI and 2355 genes for ChII. These results give us an insight into the virulence and gene content of V. mimicus, as well as constitute the first approach to its diversity.

show abstract

“…The strain V. mimicus SCCF01, isolated from diseased yellow catfish at a commercial aquaculture site in Sichuan province, China, in 2014, was identified using genome analysis ( 21 ). For the challenge trial, V. mimicus strains were incubated in BHI at 28°C for 24 hours.…”

Section: Methodsmentioning

confidence: 99%

Integrated bioinformatics identifies key mediators in cytokine storm and tissue remodeling during Vibrio mimicus infection in yellow catfish (Pelteobagrus fulvidraco)

et al. 2023

Self Cite

View full text Add to dashboard Cite

IntroductionThe pathogenesis of Vibrio mimicus infection in yellow catfish (Pelteobagrus fulvidraco) remains poorly understood, particularly regarding the impact of infection with the pathogen on primary target organs such as the skin and muscle.MethodsIn this study, we aim to analyze the pathological intricacies of the skin and muscle of yellow catfish after being infected with V. mimicus using a 1/10 LC50 seven-day post-infection model. Furthermore, we have utilized integrated bioinformatics to comprehensively elucidate the regulatory mechanisms and identify the key regulatory genes implicated in this phenomenon.ResultsOur histopathological examination revealed significant pathological changes in the skin and muscle, characterized by necrosis and inflammation. Moreover, tissue remodeling occurred, with perimysium degeneration and lesion invasion into the muscle along the endomysium, accompanied by a transformation of type I collagen into a mixture of type I and type III collagens in the perimysium and muscle bundles. Our eukaryotic transcriptomic and 4D label-free analyses demonstrated a predominantly immune pathway response in both the skin and muscle, with downregulation observed in several cell signaling pathways that focused on focal adhesion-dominated cell signaling pathways. The upregulated genes included interleukins (IL)-1 and -6, chemokines, and matrix metallopeptidases (mmp)-9 and -13, while several genes were significantly downregulated, including col1a and col1a1a. Further analysis revealed that these pathways were differentially regulated, with mmp-9 and mmp-13 acting as the potential core regulators of cytokine and tissue remodeling pathways. Upregulation of NF-κB1 and FOSL-1 induced by IL-17C and Nox 1/2-based NADPH oxidase may have held matrix metallopeptidase and cytokine-related genes. Also, we confirmed these relevant regulatory pathways by qPCR and ELISA in expanded samples.DiscussionOur findings unequivocally illustrate the occurrence of a cytokine storm and tissue remodeling, mediated by interleukins, chemokines, and MMPs, in the surface of yellow catfish infected with V. mimicus. Additionally, we unveil the potential bidirectional regulatory role of MMP-9 and MMP-13. These results provide novel perspectives on the intricate immune response to V. mimicus infection in yellow catfish and highlight potential targets for developing therapies.

show abstract

Complete genome analysis of Vibrio mimicus strain SCCF01, a highly virulent isolate from the freshwater catfish

Cited by 23 publications

References 36 publications

Occurrence of virulence determinants in vibrio cholerae, vibrio mimicus, vibrio alginolyticus, and vibrio parahaemolyticus isolates from important water resources of Eastern Cape, South Africa

Occurrence of virulence determinants in vibrio cholerae, vibrio mimicus, vibrio alginolyticus, and vibrio parahaemolyticus isolates from important water resources of Eastern Cape, South Africa

Core and Accessory Genome Analysis of Vibrio mimicus

Integrated bioinformatics identifies key mediators in cytokine storm and tissue remodeling during Vibrio mimicus infection in yellow catfish (Pelteobagrus fulvidraco)

Contact Info

Product

Resources

About